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Effects of oleic acid and chicken serum on the expression of adipogenic transcription factors and adipogenic differentiation in hen preadipocytes.
Cell Biol Int. 2013 Sep; 37(9):961-71.CB

Abstract

We have examined the effect of oleic acid (OA) concentrations and incubation time, along with chicken serum (CS), on adipogenic differentiation and expression of adipogenic transcripts in hen preadipocytes. Preadipocytes were treated with (i) an adipogenic cocktail (DMI) containing 500 nM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine and 20 µg/mL insulin alone and DMI + 75, 150, 300 or 600 µM OA for 48 h; (ii) DMI + 300 µM OA (DMIOA) for 6, 12, 24 or 48 h; and (iii) foetal bovine serum (FBS), CS, DMI + FBS, DMI + CS, DMIOA + FBS and DMIOA + CS. While FABP4 was significantly expressed with increasing concentrations of OA, the expression of C/EBPβ, LEPR and FAS were unchanged compared with the control. PPARγ2 expression was unchanged across all time-points. A significantly higher level of C/EBPα was measured at 48 h, but the levels of C/EBPβ increased after 12 h. Levels of FABP4 significantly increased with the time of incubation after 12 h, but that of LPL was reduced (P < 0.05) at 6, 24 and 48 h. FABP4 was highly expressed in cells treated with CS, DMI + CS and DMIOA + CS compared to cells treated with FBS, DMI + FBS and DMIOA + FBS. In conclusion, increased concentrations of OA and incubation time increases lipid accumulation; FABP4 and C/EBPβ are potential transcription factors regulating OA induced adipogenesis of fat cells obtained from laying hen. CS is a potent inducer of adipogenic differentiation in hen preadipocytes.

Authors+Show Affiliations

Department of Animal Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23620084

Citation

Regassa, Alemu, and Woo Kyun Kim. "Effects of Oleic Acid and Chicken Serum On the Expression of Adipogenic Transcription Factors and Adipogenic Differentiation in Hen Preadipocytes." Cell Biology International, vol. 37, no. 9, 2013, pp. 961-71.
Regassa A, Kim WK. Effects of oleic acid and chicken serum on the expression of adipogenic transcription factors and adipogenic differentiation in hen preadipocytes. Cell Biol Int. 2013;37(9):961-71.
Regassa, A., & Kim, W. K. (2013). Effects of oleic acid and chicken serum on the expression of adipogenic transcription factors and adipogenic differentiation in hen preadipocytes. Cell Biology International, 37(9), 961-71. https://doi.org/10.1002/cbin.10122
Regassa A, Kim WK. Effects of Oleic Acid and Chicken Serum On the Expression of Adipogenic Transcription Factors and Adipogenic Differentiation in Hen Preadipocytes. Cell Biol Int. 2013;37(9):961-71. PubMed PMID: 23620084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of oleic acid and chicken serum on the expression of adipogenic transcription factors and adipogenic differentiation in hen preadipocytes. AU - Regassa,Alemu, AU - Kim,Woo Kyun, Y1 - 2013/05/28/ PY - 2013/02/04/received PY - 2013/04/11/accepted PY - 2013/4/27/entrez PY - 2013/4/27/pubmed PY - 2014/2/7/medline KW - C/EBPβ KW - FABP4 KW - chicken serum KW - gene expression KW - hen preadipocytes KW - oleic acid SP - 961 EP - 71 JF - Cell biology international JO - Cell Biol Int VL - 37 IS - 9 N2 - We have examined the effect of oleic acid (OA) concentrations and incubation time, along with chicken serum (CS), on adipogenic differentiation and expression of adipogenic transcripts in hen preadipocytes. Preadipocytes were treated with (i) an adipogenic cocktail (DMI) containing 500 nM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine and 20 µg/mL insulin alone and DMI + 75, 150, 300 or 600 µM OA for 48 h; (ii) DMI + 300 µM OA (DMIOA) for 6, 12, 24 or 48 h; and (iii) foetal bovine serum (FBS), CS, DMI + FBS, DMI + CS, DMIOA + FBS and DMIOA + CS. While FABP4 was significantly expressed with increasing concentrations of OA, the expression of C/EBPβ, LEPR and FAS were unchanged compared with the control. PPARγ2 expression was unchanged across all time-points. A significantly higher level of C/EBPα was measured at 48 h, but the levels of C/EBPβ increased after 12 h. Levels of FABP4 significantly increased with the time of incubation after 12 h, but that of LPL was reduced (P < 0.05) at 6, 24 and 48 h. FABP4 was highly expressed in cells treated with CS, DMI + CS and DMIOA + CS compared to cells treated with FBS, DMI + FBS and DMIOA + FBS. In conclusion, increased concentrations of OA and incubation time increases lipid accumulation; FABP4 and C/EBPβ are potential transcription factors regulating OA induced adipogenesis of fat cells obtained from laying hen. CS is a potent inducer of adipogenic differentiation in hen preadipocytes. SN - 1095-8355 UR - https://www.unboundmedicine.com/medline/citation/23620084/Effects_of_oleic_acid_and_chicken_serum_on_the_expression_of_adipogenic_transcription_factors_and_adipogenic_differentiation_in_hen_preadipocytes_ L2 - https://doi.org/10.1002/cbin.10122 DB - PRIME DP - Unbound Medicine ER -