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Peritoneal fluid reduces angiogenesis-related microRNA expression in cell cultures of endometrial and endometriotic tissues from women with endometriosis.
PLoS One. 2013; 8(4):e62370.Plos

Abstract

Endometriosis, defined as the presence of endometrium outside the uterus, is one of the most frequent gynecological diseases. It has been suggested that modifications of both endometrial and peritoneal factors could be implicated in this disease. Endometriosis is a multifactorial disease in which angiogenesis and proteolysis are dysregulated. MicroRNAs (miRNAs) are small non-coding RNAs that regulate the protein expression and may be the main regulators of angiogenesis. Our hypothesis is that peritoneal fluid from women with endometriosis could modify the expression of several miRNAs that regulate angiogenesis and proteolysis in the endometriosis development. The objective of this study has been to evaluate the influence of endometriotic peritoneal fluid on the expression of six miRNAs related to angiogenesis, as well as several angiogenic and proteolytic factors in endometrial and endometriotic cell cultures from women with endometriosis compared with women without endometriosis.

METHODS

Endometrial and endometriotic cells were cultured and treated with endometriotic and control peritoneal fluid pools. We have studied the expression of six miRNAs (miR-16, -17-5p, -20a, -125a, -221, and -222) by RT-PCR and protein and mRNA levels of vascular endothelial growth factor-A, thrombospondin-1, urokinase plasminogen activator and plasminogen activator inhibitor-1 by ELISA and qRT-PCR respectively.

RESULTS

Control and endometriotic peritoneal fluid pools induced a significant reduction of all miRNAs levels in endometrial and endometriotic cell cultures. Moreover, both peritoneal fluids induced a significant increase in VEGF-A, uPA and PAI-1 protein levels in all cell cultures without significant increase in mRNA levels. Endometrial cell cultures from patients treated with endometriotic peritoneal fluid showed lower expression of miRNAs and higher expression of VEGF-A protein levels than cultures from controls. In conclusion , this "in vitro" study indicates that peritoneal fluid from women with endometriosis modulates the expression of miRNAs that could contribute to the angiogenic and proteolytic disequilibrium observed in this disease.

Authors+Show Affiliations

Grupo de Hemostasia, Trombosis, Arteriosclerosis y Biología Vascular, Instituto Investigación Sanitaria Hospital La Fe, Valencia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23620826

Citation

Braza-Boïls, Aitana, et al. "Peritoneal Fluid Reduces Angiogenesis-related microRNA Expression in Cell Cultures of Endometrial and Endometriotic Tissues From Women With Endometriosis." PloS One, vol. 8, no. 4, 2013, pp. e62370.
Braza-Boïls A, Gilabert-Estellés J, Ramón LA, et al. Peritoneal fluid reduces angiogenesis-related microRNA expression in cell cultures of endometrial and endometriotic tissues from women with endometriosis. PLoS ONE. 2013;8(4):e62370.
Braza-Boïls, A., Gilabert-Estellés, J., Ramón, L. A., Gilabert, J., Marí-Alexandre, J., Chirivella, M., España, F., & Estellés, A. (2013). Peritoneal fluid reduces angiogenesis-related microRNA expression in cell cultures of endometrial and endometriotic tissues from women with endometriosis. PloS One, 8(4), e62370. https://doi.org/10.1371/journal.pone.0062370
Braza-Boïls A, et al. Peritoneal Fluid Reduces Angiogenesis-related microRNA Expression in Cell Cultures of Endometrial and Endometriotic Tissues From Women With Endometriosis. PLoS ONE. 2013;8(4):e62370. PubMed PMID: 23620826.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Peritoneal fluid reduces angiogenesis-related microRNA expression in cell cultures of endometrial and endometriotic tissues from women with endometriosis. AU - Braza-Boïls,Aitana, AU - Gilabert-Estellés,Juan, AU - Ramón,Luis A, AU - Gilabert,Juan, AU - Marí-Alexandre,Josep, AU - Chirivella,Melitina, AU - España,Francisco, AU - Estellés,Amparo, Y1 - 2013/04/19/ PY - 2012/11/02/received PY - 2013/03/20/accepted PY - 2013/4/27/entrez PY - 2013/4/27/pubmed PY - 2013/11/12/medline SP - e62370 EP - e62370 JF - PloS one JO - PLoS ONE VL - 8 IS - 4 N2 - UNLABELLED: Endometriosis, defined as the presence of endometrium outside the uterus, is one of the most frequent gynecological diseases. It has been suggested that modifications of both endometrial and peritoneal factors could be implicated in this disease. Endometriosis is a multifactorial disease in which angiogenesis and proteolysis are dysregulated. MicroRNAs (miRNAs) are small non-coding RNAs that regulate the protein expression and may be the main regulators of angiogenesis. Our hypothesis is that peritoneal fluid from women with endometriosis could modify the expression of several miRNAs that regulate angiogenesis and proteolysis in the endometriosis development. The objective of this study has been to evaluate the influence of endometriotic peritoneal fluid on the expression of six miRNAs related to angiogenesis, as well as several angiogenic and proteolytic factors in endometrial and endometriotic cell cultures from women with endometriosis compared with women without endometriosis. METHODS: Endometrial and endometriotic cells were cultured and treated with endometriotic and control peritoneal fluid pools. We have studied the expression of six miRNAs (miR-16, -17-5p, -20a, -125a, -221, and -222) by RT-PCR and protein and mRNA levels of vascular endothelial growth factor-A, thrombospondin-1, urokinase plasminogen activator and plasminogen activator inhibitor-1 by ELISA and qRT-PCR respectively. RESULTS: Control and endometriotic peritoneal fluid pools induced a significant reduction of all miRNAs levels in endometrial and endometriotic cell cultures. Moreover, both peritoneal fluids induced a significant increase in VEGF-A, uPA and PAI-1 protein levels in all cell cultures without significant increase in mRNA levels. Endometrial cell cultures from patients treated with endometriotic peritoneal fluid showed lower expression of miRNAs and higher expression of VEGF-A protein levels than cultures from controls. In conclusion , this "in vitro" study indicates that peritoneal fluid from women with endometriosis modulates the expression of miRNAs that could contribute to the angiogenic and proteolytic disequilibrium observed in this disease. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23620826/Peritoneal_fluid_reduces_angiogenesis_related_microRNA_expression_in_cell_cultures_of_endometrial_and_endometriotic_tissues_from_women_with_endometriosis_ L2 - http://dx.plos.org/10.1371/journal.pone.0062370 DB - PRIME DP - Unbound Medicine ER -