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Embryonic founders of adult muscle stem cells are primed by the determination gene Mrf4.
Dev Biol. 2013 Sep 01; 381(1):241-55.DB

Abstract

Skeletal muscle satellite cells play a critical role during muscle growth, homoeostasis and regeneration. Selective induction of the muscle determination genes Myf5, Myod and Mrf4 during prenatal development can potentially impact on the reported functional heterogeneity of adult satellite cells. Accordingly, expression of Myf5 was reported to diminish the self-renewal potential of the majority of satellite cells. In contrast, virtually all adult satellite cells showed antecedence of Myod activity. Here we examine the priming of myogenic cells by Mrf4 throughout development. Using a Cre-lox based genetic strategy and novel highly sensitive Pax7 reporter alleles compared to the ubiquitous Rosa26-based reporters, we show that all adult satellite cells, independently of their anatomical location or embryonic origin, have been primed for Mrf4 expression. Given that Mrf4Cre and Mrf4nlacZ are active exclusively in progenitors during embryogenesis, whereas later expression is restricted to differentiated myogenic cells, our findings suggest that adult satellite cells emerge from embryonic founder cells in which the Mrf4 locus was activated. Therefore, this level of myogenic priming by induction of Mrf4, does not compromise the potential of the founder cells to assume an upstream muscle stem cell state. We propose that embryonic myogenic cells and the majority of adult muscle stem cells form a lineage continuum.

Authors+Show Affiliations

Institut Pasteur, Stem Cells & Development, CNRS URA 2578, 25 rue du Dr. Roux, 75724, Paris Cedex 15, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23623977

Citation

Sambasivan, Ramkumar, et al. "Embryonic Founders of Adult Muscle Stem Cells Are Primed By the Determination Gene Mrf4." Developmental Biology, vol. 381, no. 1, 2013, pp. 241-55.
Sambasivan R, Comai G, Le Roux I, et al. Embryonic founders of adult muscle stem cells are primed by the determination gene Mrf4. Dev Biol. 2013;381(1):241-55.
Sambasivan, R., Comai, G., Le Roux, I., Gomès, D., Konge, J., Dumas, G., Cimper, C., & Tajbakhsh, S. (2013). Embryonic founders of adult muscle stem cells are primed by the determination gene Mrf4. Developmental Biology, 381(1), 241-55. https://doi.org/10.1016/j.ydbio.2013.04.018
Sambasivan R, et al. Embryonic Founders of Adult Muscle Stem Cells Are Primed By the Determination Gene Mrf4. Dev Biol. 2013 Sep 1;381(1):241-55. PubMed PMID: 23623977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Embryonic founders of adult muscle stem cells are primed by the determination gene Mrf4. AU - Sambasivan,Ramkumar, AU - Comai,Glenda, AU - Le Roux,Isabelle, AU - Gomès,Danielle, AU - Konge,Julie, AU - Dumas,Gérard, AU - Cimper,Clémire, AU - Tajbakhsh,Shahragim, Y1 - 2013/04/25/ PY - 2012/10/02/received PY - 2013/03/27/revised PY - 2013/04/17/accepted PY - 2013/4/30/entrez PY - 2013/4/30/pubmed PY - 2013/10/29/medline KW - Lineage KW - Mrf4 KW - Myf5 KW - Pax7 KW - Reporter mice KW - Skeletal muscle stem cells SP - 241 EP - 55 JF - Developmental biology JO - Dev Biol VL - 381 IS - 1 N2 - Skeletal muscle satellite cells play a critical role during muscle growth, homoeostasis and regeneration. Selective induction of the muscle determination genes Myf5, Myod and Mrf4 during prenatal development can potentially impact on the reported functional heterogeneity of adult satellite cells. Accordingly, expression of Myf5 was reported to diminish the self-renewal potential of the majority of satellite cells. In contrast, virtually all adult satellite cells showed antecedence of Myod activity. Here we examine the priming of myogenic cells by Mrf4 throughout development. Using a Cre-lox based genetic strategy and novel highly sensitive Pax7 reporter alleles compared to the ubiquitous Rosa26-based reporters, we show that all adult satellite cells, independently of their anatomical location or embryonic origin, have been primed for Mrf4 expression. Given that Mrf4Cre and Mrf4nlacZ are active exclusively in progenitors during embryogenesis, whereas later expression is restricted to differentiated myogenic cells, our findings suggest that adult satellite cells emerge from embryonic founder cells in which the Mrf4 locus was activated. Therefore, this level of myogenic priming by induction of Mrf4, does not compromise the potential of the founder cells to assume an upstream muscle stem cell state. We propose that embryonic myogenic cells and the majority of adult muscle stem cells form a lineage continuum. SN - 1095-564X UR - https://www.unboundmedicine.com/medline/citation/23623977/Embryonic_founders_of_adult_muscle_stem_cells_are_primed_by_the_determination_gene_Mrf4_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(13)00198-X DB - PRIME DP - Unbound Medicine ER -