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Role of IL-22- and TNF-α-producing Th22 cells in uveitis patients with Behcet's disease.
J Immunol. 2013 Jun 01; 190(11):5799-808.JI

Abstract

Behçet's disease is a systemic inflammatory disorder with recurrent episodes of oral ulceration, skin lesions, genital ulceration, and intraocular inflammation (uveitis). The intraocular inflammation is strictly associated with Th effector cells. IL-22 is a member of the IL-10 cytokine family that is involved in inflammatory processes. Recently, Th22 cells were identified as a Th cell population that produces IL-22 and TNF-α and are distinct from Th1, Th2, and Th17 cells. In this study, we established Th22-type T cell clones from ocular samples taken from Behçet's disease patients with active uveitis. These clones produced large amounts of IL-22 and TNF-α but not the Th1 cytokine IFN-γ and the Th17 cytokine IL-17. CD4(+) T cells from the peripheral blood of Behçet's disease patients differentiated into Th22 cells in the presence of IL-6 and TNF-α in vitro. The polarized Th22 cell lines produced large amounts of IL-22, and the polarized Th1 and Th17 cells also produced IL-22. In the presence of anti-TNF-α- and anti-IL-6-blocking Abs, Behçet's disease Th22-type T cells failed to produce IL-22. In addition, infliximab-pretreated Th22 cells and Th22-type ocular T cells produced less IL-22 and TNF-α. Moreover, IL-22-producing T cells were isolated from mice with experimental autoimmune uveitis, an animal model of Behçet's disease, and the intraocular T cells from uveitis models produced large amounts of IL-22 in the presence of retinal Ags. Our results suggest that inflammatory cytokines IL-22 and TNF-α may play a key role in the ocular immune response in Behçet's disease.

Authors+Show Affiliations

Department of Ophthalmology and Visual Science, Tokyo Medical and Dental University Graduate School of Medicine and Dental Sciences, Tokyo 113-8519, Japan. sunaoph@cdb.riken.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23630362

Citation

Sugita, Sunao, et al. "Role of IL-22- and TNF-α-producing Th22 Cells in Uveitis Patients With Behcet's Disease." Journal of Immunology (Baltimore, Md. : 1950), vol. 190, no. 11, 2013, pp. 5799-808.
Sugita S, Kawazoe Y, Imai A, et al. Role of IL-22- and TNF-α-producing Th22 cells in uveitis patients with Behcet's disease. J Immunol. 2013;190(11):5799-808.
Sugita, S., Kawazoe, Y., Imai, A., Kawaguchi, T., Horie, S., Keino, H., Takahashi, M., & Mochizuki, M. (2013). Role of IL-22- and TNF-α-producing Th22 cells in uveitis patients with Behcet's disease. Journal of Immunology (Baltimore, Md. : 1950), 190(11), 5799-808. https://doi.org/10.4049/jimmunol.1202677
Sugita S, et al. Role of IL-22- and TNF-α-producing Th22 Cells in Uveitis Patients With Behcet's Disease. J Immunol. 2013 Jun 1;190(11):5799-808. PubMed PMID: 23630362.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of IL-22- and TNF-α-producing Th22 cells in uveitis patients with Behcet's disease. AU - Sugita,Sunao, AU - Kawazoe,Yuko, AU - Imai,Ayano, AU - Kawaguchi,Tatsushi, AU - Horie,Shintaro, AU - Keino,Hiroshi, AU - Takahashi,Masayo, AU - Mochizuki,Manabu, Y1 - 2013/04/29/ PY - 2013/5/1/entrez PY - 2013/5/1/pubmed PY - 2013/7/24/medline SP - 5799 EP - 808 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 190 IS - 11 N2 - Behçet's disease is a systemic inflammatory disorder with recurrent episodes of oral ulceration, skin lesions, genital ulceration, and intraocular inflammation (uveitis). The intraocular inflammation is strictly associated with Th effector cells. IL-22 is a member of the IL-10 cytokine family that is involved in inflammatory processes. Recently, Th22 cells were identified as a Th cell population that produces IL-22 and TNF-α and are distinct from Th1, Th2, and Th17 cells. In this study, we established Th22-type T cell clones from ocular samples taken from Behçet's disease patients with active uveitis. These clones produced large amounts of IL-22 and TNF-α but not the Th1 cytokine IFN-γ and the Th17 cytokine IL-17. CD4(+) T cells from the peripheral blood of Behçet's disease patients differentiated into Th22 cells in the presence of IL-6 and TNF-α in vitro. The polarized Th22 cell lines produced large amounts of IL-22, and the polarized Th1 and Th17 cells also produced IL-22. In the presence of anti-TNF-α- and anti-IL-6-blocking Abs, Behçet's disease Th22-type T cells failed to produce IL-22. In addition, infliximab-pretreated Th22 cells and Th22-type ocular T cells produced less IL-22 and TNF-α. Moreover, IL-22-producing T cells were isolated from mice with experimental autoimmune uveitis, an animal model of Behçet's disease, and the intraocular T cells from uveitis models produced large amounts of IL-22 in the presence of retinal Ags. Our results suggest that inflammatory cytokines IL-22 and TNF-α may play a key role in the ocular immune response in Behçet's disease. SN - 1550-6606 UR - https://www.unboundmedicine.com/medline/citation/23630362/Role_of_IL_22__and_TNF_α_producing_Th22_cells_in_uveitis_patients_with_Behcet's_disease_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=23630362 DB - PRIME DP - Unbound Medicine ER -