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Biochemical composition, antimicrobial activities,and anti-quorum-sensing activities of ethanol and ethyl acetate extracts from Hypericum connatum Lam. (Guttiferae).
J Med Food. 2013 May; 16(5):454-9.JM

Abstract

We studied the polyphenol composition and antioxidant activity of the ethanolic and ethyl acetate extracts of Hypericum connatum (Guttiferae), in addition to their antimicrobial and antiquorum-sensing (AQS) properties. The extracts exhibited a similar polyphenol content and a notable antioxidant activity, which was stronger in the ethanolic extract (EC50=3.2 μg/mL). Ultrahigh-performance liquid chromatography analyses revealed different polyphenol profiles, with rutin and apigen being the main components of the ethanolic extract and caffeic acid, (-)-epicatechin and p-coumaric acid being abundant in the ethyl acetate extract. The ethyl acetate extract exhibited a more effective antimicrobial activity than the ethanolic extract against the pathogens Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa. For the first time, AQS activity was detected in H. connatum: the ethanolic extract inhibited the production of violacein, a quorum-sensing-regulated pigment, in a Chromobacterium violaceum tester strain, without interfering with its growth.

Authors+Show Affiliations

Institute of Food Science, National Research Council ISA-CNR, Avellino, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23631492

Citation

Fratianni, Florinda, et al. "Biochemical Composition, Antimicrobial Activities,and Anti-quorum-sensing Activities of Ethanol and Ethyl Acetate Extracts From Hypericum Connatum Lam. (Guttiferae)." Journal of Medicinal Food, vol. 16, no. 5, 2013, pp. 454-9.
Fratianni F, Nazzaro F, Marandino A, et al. Biochemical composition, antimicrobial activities,and anti-quorum-sensing activities of ethanol and ethyl acetate extracts from Hypericum connatum Lam. (Guttiferae). J Med Food. 2013;16(5):454-9.
Fratianni, F., Nazzaro, F., Marandino, A., Fusco, M. d. e. l. . R., Coppola, R., De Feo, V., & De Martino, L. (2013). Biochemical composition, antimicrobial activities,and anti-quorum-sensing activities of ethanol and ethyl acetate extracts from Hypericum connatum Lam. (Guttiferae). Journal of Medicinal Food, 16(5), 454-9. https://doi.org/10.1089/jmf.2012.0197
Fratianni F, et al. Biochemical Composition, Antimicrobial Activities,and Anti-quorum-sensing Activities of Ethanol and Ethyl Acetate Extracts From Hypericum Connatum Lam. (Guttiferae). J Med Food. 2013;16(5):454-9. PubMed PMID: 23631492.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biochemical composition, antimicrobial activities,and anti-quorum-sensing activities of ethanol and ethyl acetate extracts from Hypericum connatum Lam. (Guttiferae). AU - Fratianni,Florinda, AU - Nazzaro,Filomena, AU - Marandino,Aurelio, AU - Fusco,Maria Del Rosario, AU - Coppola,Raffaele, AU - De Feo,Vincenzo, AU - De Martino,Laura, Y1 - 2013/04/30/ PY - 2013/5/2/entrez PY - 2013/5/2/pubmed PY - 2013/12/18/medline SP - 454 EP - 9 JF - Journal of medicinal food JO - J Med Food VL - 16 IS - 5 N2 - We studied the polyphenol composition and antioxidant activity of the ethanolic and ethyl acetate extracts of Hypericum connatum (Guttiferae), in addition to their antimicrobial and antiquorum-sensing (AQS) properties. The extracts exhibited a similar polyphenol content and a notable antioxidant activity, which was stronger in the ethanolic extract (EC50=3.2 μg/mL). Ultrahigh-performance liquid chromatography analyses revealed different polyphenol profiles, with rutin and apigen being the main components of the ethanolic extract and caffeic acid, (-)-epicatechin and p-coumaric acid being abundant in the ethyl acetate extract. The ethyl acetate extract exhibited a more effective antimicrobial activity than the ethanolic extract against the pathogens Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa. For the first time, AQS activity was detected in H. connatum: the ethanolic extract inhibited the production of violacein, a quorum-sensing-regulated pigment, in a Chromobacterium violaceum tester strain, without interfering with its growth. SN - 1557-7600 UR - https://www.unboundmedicine.com/medline/citation/23631492/Biochemical_composition_antimicrobial_activitiesand_anti_quorum_sensing_activities_of_ethanol_and_ethyl_acetate_extracts_from_Hypericum_connatum_Lam___Guttiferae__ L2 - https://www.liebertpub.com/doi/full/10.1089/jmf.2012.0197?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -