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Protein array of Coxiella burnetii probed with Q fever sera.
Sci China Life Sci. 2013 May; 56(5):453-9.SC

Abstract

Coxiella burnetii is the etiological agent of Q fever. To identify its major seroreactive proteins, a subgenomic protein array was developed. A total of 101 assumed virulence-associated recombinant proteins of C. burnetii were probed with sera from mice experimentally infected with C. burnetii and sera from Q fever patients. Sixteen proteins were recognized as major seroreactive antigens by the mouse sera. Seven of these 16 proteins reacted positively with at least 45% of Q fever patient sera. Notably, HspB had the highest fluorescence intensity value and positive frequency of all the proteins on the array when probed with both Q fever patient sera and mouse sera. These results suggest that these seven major seroreactive proteins, particularly HspB, are potential serodiagnostic and subunit vaccine antigens of Q fever.

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Authors+Show Affiliations

Wang X
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Xiong X
No affiliation info available
Graves S
No affiliation info available
Stenos J
No affiliation info available
Wen B
No affiliation info available

MeSH

AnimalsAntibodies, BacterialAntigens, BacterialBacterial ProteinsBacterial VaccinesCoxiella burnetiiHeat-Shock ProteinsHumansMaleMiceMice, Inbred BALB CQ FeverRecombinant Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23633077

Citation

Wang, Xile, et al. "Protein Array of Coxiella Burnetii Probed With Q Fever Sera." Science China. Life Sciences, vol. 56, no. 5, 2013, pp. 453-9.
Wang X, Xiong X, Graves S, et al. Protein array of Coxiella burnetii probed with Q fever sera. Sci China Life Sci. 2013;56(5):453-9.
Wang, X., Xiong, X., Graves, S., Stenos, J., & Wen, B. (2013). Protein array of Coxiella burnetii probed with Q fever sera. Science China. Life Sciences, 56(5), 453-9. https://doi.org/10.1007/s11427-013-4472-6
Wang X, et al. Protein Array of Coxiella Burnetii Probed With Q Fever Sera. Sci China Life Sci. 2013;56(5):453-9. PubMed PMID: 23633077.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein array of Coxiella burnetii probed with Q fever sera. AU - Wang,Xile, AU - Xiong,Xiaolu, AU - Graves,Stephen, AU - Stenos,John, AU - Wen,Bohai, Y1 - 2013/05/01/ PY - 2012/10/12/received PY - 2013/03/21/accepted PY - 2013/5/2/entrez PY - 2013/5/2/pubmed PY - 2013/12/16/medline SP - 453 EP - 9 JF - Science China. Life sciences JO - Sci China Life Sci VL - 56 IS - 5 N2 - Coxiella burnetii is the etiological agent of Q fever. To identify its major seroreactive proteins, a subgenomic protein array was developed. A total of 101 assumed virulence-associated recombinant proteins of C. burnetii were probed with sera from mice experimentally infected with C. burnetii and sera from Q fever patients. Sixteen proteins were recognized as major seroreactive antigens by the mouse sera. Seven of these 16 proteins reacted positively with at least 45% of Q fever patient sera. Notably, HspB had the highest fluorescence intensity value and positive frequency of all the proteins on the array when probed with both Q fever patient sera and mouse sera. These results suggest that these seven major seroreactive proteins, particularly HspB, are potential serodiagnostic and subunit vaccine antigens of Q fever. SN - 1869-1889 UR - https://www.unboundmedicine.com/medline/citation/23633077/Protein_array_of_Coxiella_burnetii_probed_with_Q_fever_sera_ L2 - https://dx.doi.org/10.1007/s11427-013-4472-6 DB - PRIME DP - Unbound Medicine ER -