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Protein array of Coxiella burnetii probed with Q fever sera.
Sci China Life Sci. 2013 May; 56(5):453-9.SC

Abstract

Coxiella burnetii is the etiological agent of Q fever. To identify its major seroreactive proteins, a subgenomic protein array was developed. A total of 101 assumed virulence-associated recombinant proteins of C. burnetii were probed with sera from mice experimentally infected with C. burnetii and sera from Q fever patients. Sixteen proteins were recognized as major seroreactive antigens by the mouse sera. Seven of these 16 proteins reacted positively with at least 45% of Q fever patient sera. Notably, HspB had the highest fluorescence intensity value and positive frequency of all the proteins on the array when probed with both Q fever patient sera and mouse sera. These results suggest that these seven major seroreactive proteins, particularly HspB, are potential serodiagnostic and subunit vaccine antigens of Q fever.

Authors+Show Affiliations

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23633077

Citation

Wang, Xile, et al. "Protein Array of Coxiella Burnetii Probed With Q Fever Sera." Science China. Life Sciences, vol. 56, no. 5, 2013, pp. 453-9.
Wang X, Xiong X, Graves S, et al. Protein array of Coxiella burnetii probed with Q fever sera. Sci China Life Sci. 2013;56(5):453-9.
Wang, X., Xiong, X., Graves, S., Stenos, J., & Wen, B. (2013). Protein array of Coxiella burnetii probed with Q fever sera. Science China. Life Sciences, 56(5), 453-9. https://doi.org/10.1007/s11427-013-4472-6
Wang X, et al. Protein Array of Coxiella Burnetii Probed With Q Fever Sera. Sci China Life Sci. 2013;56(5):453-9. PubMed PMID: 23633077.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein array of Coxiella burnetii probed with Q fever sera. AU - Wang,Xile, AU - Xiong,Xiaolu, AU - Graves,Stephen, AU - Stenos,John, AU - Wen,Bohai, Y1 - 2013/05/01/ PY - 2012/10/12/received PY - 2013/03/21/accepted PY - 2013/5/2/entrez PY - 2013/5/2/pubmed PY - 2013/12/16/medline SP - 453 EP - 9 JF - Science China. Life sciences JO - Sci China Life Sci VL - 56 IS - 5 N2 - Coxiella burnetii is the etiological agent of Q fever. To identify its major seroreactive proteins, a subgenomic protein array was developed. A total of 101 assumed virulence-associated recombinant proteins of C. burnetii were probed with sera from mice experimentally infected with C. burnetii and sera from Q fever patients. Sixteen proteins were recognized as major seroreactive antigens by the mouse sera. Seven of these 16 proteins reacted positively with at least 45% of Q fever patient sera. Notably, HspB had the highest fluorescence intensity value and positive frequency of all the proteins on the array when probed with both Q fever patient sera and mouse sera. These results suggest that these seven major seroreactive proteins, particularly HspB, are potential serodiagnostic and subunit vaccine antigens of Q fever. SN - 1869-1889 UR - https://www.unboundmedicine.com/medline/citation/23633077/Protein_array_of_Coxiella_burnetii_probed_with_Q_fever_sera_ L2 - https://dx.doi.org/10.1007/s11427-013-4472-6 DB - PRIME DP - Unbound Medicine ER -