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Six homeoproteins directly activate Myod expression in the gene regulatory networks that control early myogenesis.
PLoS Genet. 2013 Apr; 9(4):e1003425.PG

Abstract

In mammals, several genetic pathways have been characterized that govern engagement of multipotent embryonic progenitors into the myogenic program through the control of the key myogenic regulatory gene Myod. Here we demonstrate the involvement of Six homeoproteins. We first targeted into a Pax3 allele a sequence encoding a negative form of Six4 that binds DNA but cannot interact with essential Eya co-factors. The resulting embryos present hypoplasic skeletal muscles and impaired Myod activation in the trunk in the absence of Myf5/Mrf4. At the axial level, we further show that Myod is still expressed in compound Six1/Six4:Pax3 but not in Six1/Six4:Myf5 triple mutant embryos, demonstrating that Six1/4 participates in the Pax3-Myod genetic pathway. Myod expression and head myogenesis is preserved in Six1/Six4:Myf5 triple mutant embryos, illustrating that upstream regulators of Myod in different embryonic territories are distinct. We show that Myod regulatory regions are directly controlled by Six proteins and that, in the absence of Six1 and Six4, Six2 can compensate.

Authors+Show Affiliations

UPMC Paris 06, UMR-S 787, Paris, France. frelaix@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23637613

Citation

Relaix, Frédéric, et al. "Six Homeoproteins Directly Activate Myod Expression in the Gene Regulatory Networks That Control Early Myogenesis." PLoS Genetics, vol. 9, no. 4, 2013, pp. e1003425.
Relaix F, Demignon J, Laclef C, et al. Six homeoproteins directly activate Myod expression in the gene regulatory networks that control early myogenesis. PLoS Genet. 2013;9(4):e1003425.
Relaix, F., Demignon, J., Laclef, C., Pujol, J., Santolini, M., Niro, C., Lagha, M., Rocancourt, D., Buckingham, M., & Maire, P. (2013). Six homeoproteins directly activate Myod expression in the gene regulatory networks that control early myogenesis. PLoS Genetics, 9(4), e1003425. https://doi.org/10.1371/journal.pgen.1003425
Relaix F, et al. Six Homeoproteins Directly Activate Myod Expression in the Gene Regulatory Networks That Control Early Myogenesis. PLoS Genet. 2013;9(4):e1003425. PubMed PMID: 23637613.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Six homeoproteins directly activate Myod expression in the gene regulatory networks that control early myogenesis. AU - Relaix,Frédéric, AU - Demignon,Josiane, AU - Laclef,Christine, AU - Pujol,Julien, AU - Santolini,Marc, AU - Niro,Claire, AU - Lagha,Mounia, AU - Rocancourt,Didier, AU - Buckingham,Margaret, AU - Maire,Pascal, Y1 - 2013/04/25/ PY - 2012/08/28/received PY - 2013/02/14/accepted PY - 2013/5/3/entrez PY - 2013/5/3/pubmed PY - 2016/4/23/medline SP - e1003425 EP - e1003425 JF - PLoS genetics JO - PLoS Genet. VL - 9 IS - 4 N2 - In mammals, several genetic pathways have been characterized that govern engagement of multipotent embryonic progenitors into the myogenic program through the control of the key myogenic regulatory gene Myod. Here we demonstrate the involvement of Six homeoproteins. We first targeted into a Pax3 allele a sequence encoding a negative form of Six4 that binds DNA but cannot interact with essential Eya co-factors. The resulting embryos present hypoplasic skeletal muscles and impaired Myod activation in the trunk in the absence of Myf5/Mrf4. At the axial level, we further show that Myod is still expressed in compound Six1/Six4:Pax3 but not in Six1/Six4:Myf5 triple mutant embryos, demonstrating that Six1/4 participates in the Pax3-Myod genetic pathway. Myod expression and head myogenesis is preserved in Six1/Six4:Myf5 triple mutant embryos, illustrating that upstream regulators of Myod in different embryonic territories are distinct. We show that Myod regulatory regions are directly controlled by Six proteins and that, in the absence of Six1 and Six4, Six2 can compensate. SN - 1553-7404 UR - https://www.unboundmedicine.com/medline/citation/23637613/Six_homeoproteins_directly_activate_Myod_expression_in_the_gene_regulatory_networks_that_control_early_myogenesis_ L2 - http://dx.plos.org/10.1371/journal.pgen.1003425 DB - PRIME DP - Unbound Medicine ER -