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Myf5 expression during fetal myogenesis defines the developmental progenitors of adult satellite cells.
Dev Biol. 2013 Jul 15; 379(2):195-207.DB

Abstract

Myf5 is a member of the muscle-specific determination genes and plays a critical role in skeletal muscle development. Whereas the expression of Myf5 during embryonic and fetal myogenesis has been extensively studied, its expression in progenitors that will ultimately give rise to adult satellite cells, the stem cells responsible for muscle repair, is still largely unexplored. To investigate this aspect, we have generated a mouse strain carrying a CreER coding sequence in the Myf5 locus. In this strain, Tamoxifen-inducible Cre activity parallels endogenous Myf5 expression. Combining Myf5(CreER) and Cre reporter alleles, we were able to evaluate the contribution of cells expressing Myf5 at distinct developmental stages to the pool of satellite cells in adult hindlimb muscles. Although it was possible to trace back the origin of some rare satellite cells to a subpopulation of Myf5(+ve) progenitors in the limb buds at the late embryonic stage (∼E12), a significant number of satellite cells arise from cells which expressed Myf5 for the first time at the fetal stage (∼E15). These studies provide direct evidence that adult satellite cells derive from progenitors that first express the myogenic determination gene Myf5 during fetal stages of myogenesis.

Authors+Show Affiliations

Paul F. Glenn Laboratories for the Biology of Aging and Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23639729

Citation

Biressi, Stefano, et al. "Myf5 Expression During Fetal Myogenesis Defines the Developmental Progenitors of Adult Satellite Cells." Developmental Biology, vol. 379, no. 2, 2013, pp. 195-207.
Biressi S, Bjornson CR, Carlig PM, et al. Myf5 expression during fetal myogenesis defines the developmental progenitors of adult satellite cells. Dev Biol. 2013;379(2):195-207.
Biressi, S., Bjornson, C. R., Carlig, P. M., Nishijo, K., Keller, C., & Rando, T. A. (2013). Myf5 expression during fetal myogenesis defines the developmental progenitors of adult satellite cells. Developmental Biology, 379(2), 195-207. https://doi.org/10.1016/j.ydbio.2013.04.021
Biressi S, et al. Myf5 Expression During Fetal Myogenesis Defines the Developmental Progenitors of Adult Satellite Cells. Dev Biol. 2013 Jul 15;379(2):195-207. PubMed PMID: 23639729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Myf5 expression during fetal myogenesis defines the developmental progenitors of adult satellite cells. AU - Biressi,Stefano, AU - Bjornson,Christopher R R, AU - Carlig,Poppy M M, AU - Nishijo,Koichi, AU - Keller,Charles, AU - Rando,Thomas A, Y1 - 2013/04/29/ PY - 2012/09/05/received PY - 2013/03/21/revised PY - 2013/04/09/accepted PY - 2013/5/4/entrez PY - 2013/5/4/pubmed PY - 2013/8/14/medline SP - 195 EP - 207 JF - Developmental biology JO - Dev Biol VL - 379 IS - 2 N2 - Myf5 is a member of the muscle-specific determination genes and plays a critical role in skeletal muscle development. Whereas the expression of Myf5 during embryonic and fetal myogenesis has been extensively studied, its expression in progenitors that will ultimately give rise to adult satellite cells, the stem cells responsible for muscle repair, is still largely unexplored. To investigate this aspect, we have generated a mouse strain carrying a CreER coding sequence in the Myf5 locus. In this strain, Tamoxifen-inducible Cre activity parallels endogenous Myf5 expression. Combining Myf5(CreER) and Cre reporter alleles, we were able to evaluate the contribution of cells expressing Myf5 at distinct developmental stages to the pool of satellite cells in adult hindlimb muscles. Although it was possible to trace back the origin of some rare satellite cells to a subpopulation of Myf5(+ve) progenitors in the limb buds at the late embryonic stage (∼E12), a significant number of satellite cells arise from cells which expressed Myf5 for the first time at the fetal stage (∼E15). These studies provide direct evidence that adult satellite cells derive from progenitors that first express the myogenic determination gene Myf5 during fetal stages of myogenesis. SN - 1095-564X UR - https://www.unboundmedicine.com/medline/citation/23639729/Myf5_expression_during_fetal_myogenesis_defines_the_developmental_progenitors_of_adult_satellite_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(13)00201-7 DB - PRIME DP - Unbound Medicine ER -