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Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation.
Magn Reson Imaging. 2013 Sep; 31(7):1174-81.MR

Abstract

PURPOSE

This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation.

MATERIALS AND METHODS

Following surgical induction of MCAO for 90min, agmatine was injected 5min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3h-72h in a 1.5T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections.

RESULTS

Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3h and peaked at 24h-48h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72h) point except for significantly smaller ADC lesions in the MCAO model (P<0.05). Volumetric calculation based on TTC-derived infarct also correlated significantly stronger to volumetric calculation based on last imaging time point derived on T2WI, DWI or CE-T1WI than ADC (P<0.05). At the last imaging time point, a significant increase in Evans Blue extravasation and a significant decrease in Nissl-positive cells numbers were noted in the vehicle-treated MCAO injured animals. The lesion volumes derived from T2WI, DWI, CE-T1WI, and Evans blue extravasation as well as the reduced numbers of Nissl-positive cells were all significantly attenuated in the agmatine-treated rats compared with the control ischemia rats (P<0.05).

CONCLUSION

Our results suggest that agmatine has neuroprotective effects against brain edema on a reperfusion model after transient cerebral ischemia.

Authors+Show Affiliations

Department of Radiology, Chi Mei Medical Center, Liouying, Tainan, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23642800

Citation

Huang, Y C., et al. "Neuroprotective Effect of Agmatine in Rats With Transient Cerebral Ischemia Using MR Imaging and Histopathologic Evaluation." Magnetic Resonance Imaging, vol. 31, no. 7, 2013, pp. 1174-81.
Huang YC, Tzeng WS, Wang CC, et al. Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation. Magn Reson Imaging. 2013;31(7):1174-81.
Huang, Y. C., Tzeng, W. S., Wang, C. C., Cheng, B. C., Chang, Y. K., Chen, H. H., Lin, P. C., Huang, T. Y., Chuang, T. J., Lin, J. W., & Chang, C. P. (2013). Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation. Magnetic Resonance Imaging, 31(7), 1174-81. https://doi.org/10.1016/j.mri.2013.03.026
Huang YC, et al. Neuroprotective Effect of Agmatine in Rats With Transient Cerebral Ischemia Using MR Imaging and Histopathologic Evaluation. Magn Reson Imaging. 2013;31(7):1174-81. PubMed PMID: 23642800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation. AU - Huang,Y C, AU - Tzeng,W S, AU - Wang,C C, AU - Cheng,B C, AU - Chang,Y K, AU - Chen,H H, AU - Lin,P C, AU - Huang,T Y, AU - Chuang,T J, AU - Lin,J W, AU - Chang,C P, Y1 - 2013/05/01/ PY - 2012/09/06/received PY - 2013/03/28/revised PY - 2013/03/28/accepted PY - 2013/5/7/entrez PY - 2013/5/7/pubmed PY - 2014/2/22/medline KW - ADC KW - ANOVA KW - AQP4 KW - Agmatine KW - BBB KW - Brain edema KW - CE-T1WI KW - DWI KW - EB KW - Evans blue KW - MCAO KW - MRI KW - Magnetic resonance image KW - PBS KW - Pharmacology KW - ROI KW - Stroke KW - T2-weighted images KW - T2WI KW - TTC KW - analysis of variance KW - apparent diffusion coefficient KW - aquaporin 4 KW - blood–brain-barrier KW - contrast-enhanced T1-weighted images KW - diffusion-weighted images KW - magnetic resonance imaging KW - middle cerebral artery occlusion KW - phosphate-buffered saline KW - region of interest KW - triphenyltetrazolium chloride SP - 1174 EP - 81 JF - Magnetic resonance imaging JO - Magn Reson Imaging VL - 31 IS - 7 N2 - PURPOSE: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. MATERIALS AND METHODS: Following surgical induction of MCAO for 90min, agmatine was injected 5min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3h-72h in a 1.5T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. RESULTS: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3h and peaked at 24h-48h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72h) point except for significantly smaller ADC lesions in the MCAO model (P<0.05). Volumetric calculation based on TTC-derived infarct also correlated significantly stronger to volumetric calculation based on last imaging time point derived on T2WI, DWI or CE-T1WI than ADC (P<0.05). At the last imaging time point, a significant increase in Evans Blue extravasation and a significant decrease in Nissl-positive cells numbers were noted in the vehicle-treated MCAO injured animals. The lesion volumes derived from T2WI, DWI, CE-T1WI, and Evans blue extravasation as well as the reduced numbers of Nissl-positive cells were all significantly attenuated in the agmatine-treated rats compared with the control ischemia rats (P<0.05). CONCLUSION: Our results suggest that agmatine has neuroprotective effects against brain edema on a reperfusion model after transient cerebral ischemia. SN - 1873-5894 UR - https://www.unboundmedicine.com/medline/citation/23642800/Neuroprotective_effect_of_agmatine_in_rats_with_transient_cerebral_ischemia_using_MR_imaging_and_histopathologic_evaluation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0730-725X(13)00133-1 DB - PRIME DP - Unbound Medicine ER -