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Ionic liquid functionalized graphene/Au nanocomposites and its application for electrochemical immunosensor.
Biosens Bioelectron. 2013 Oct 15; 48:33-8.BB

Abstract

In this work, a new nanocomposite, which was composed of ionic liquid functionalized graphene sheet (IL-GS) loaded gold nanoparticles (AuNPs), was prepared. The IL-GS was directly synthesized by the electrochemical exfoliation of graphite in ionic liquid (IL). Due to the modification of the IL, IL-GS can not only be dispersed easily in aqueous solution to form a homogeneous colloidal suspension of individual sheet, but also exhibits an improved conductivity. Meanwhile, the loaded AuNPs on the nanocomposites can increase the specific surface area to capture a large amount of antibodies as well as improve the capability of electron transfer. The IL-GS-Au nanocomposites were successfully employed for the fabrication of a facile and sensitive electrochemical immunosensor. Carcinoembryonic antigen (CEA) was used as a model protein. The proposed immunosensor exhibits a wide linear detection range (LDR) from 1 fg mL⁻¹ to 100 ng mL⁻¹, and an ultralow limit of detection (LOD) of 0.1 fg mL⁻¹ (S/N=3). In addition, for the detection of clinical serum samples, it is well consistent with the data determined by the developed immunoassay and ELISA, indicating that the immunosensor provides a possible application for the detection of CEA in clinical diagnostics.

Authors+Show Affiliations

College of Life Science, Capital Normal University, Beijing 100048, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23644143

Citation

Liu, Na, et al. "Ionic Liquid Functionalized graphene/Au Nanocomposites and Its Application for Electrochemical Immunosensor." Biosensors & Bioelectronics, vol. 48, 2013, pp. 33-8.
Liu N, Chen X, Ma Z. Ionic liquid functionalized graphene/Au nanocomposites and its application for electrochemical immunosensor. Biosens Bioelectron. 2013;48:33-8.
Liu, N., Chen, X., & Ma, Z. (2013). Ionic liquid functionalized graphene/Au nanocomposites and its application for electrochemical immunosensor. Biosensors & Bioelectronics, 48, 33-8. https://doi.org/10.1016/j.bios.2013.03.080
Liu N, Chen X, Ma Z. Ionic Liquid Functionalized graphene/Au Nanocomposites and Its Application for Electrochemical Immunosensor. Biosens Bioelectron. 2013 Oct 15;48:33-8. PubMed PMID: 23644143.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ionic liquid functionalized graphene/Au nanocomposites and its application for electrochemical immunosensor. AU - Liu,Na, AU - Chen,Xia, AU - Ma,Zhanfang, Y1 - 2013/04/11/ PY - 2013/01/19/received PY - 2013/03/16/revised PY - 2013/03/28/accepted PY - 2013/5/7/entrez PY - 2013/5/7/pubmed PY - 2014/1/3/medline SP - 33 EP - 8 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 48 N2 - In this work, a new nanocomposite, which was composed of ionic liquid functionalized graphene sheet (IL-GS) loaded gold nanoparticles (AuNPs), was prepared. The IL-GS was directly synthesized by the electrochemical exfoliation of graphite in ionic liquid (IL). Due to the modification of the IL, IL-GS can not only be dispersed easily in aqueous solution to form a homogeneous colloidal suspension of individual sheet, but also exhibits an improved conductivity. Meanwhile, the loaded AuNPs on the nanocomposites can increase the specific surface area to capture a large amount of antibodies as well as improve the capability of electron transfer. The IL-GS-Au nanocomposites were successfully employed for the fabrication of a facile and sensitive electrochemical immunosensor. Carcinoembryonic antigen (CEA) was used as a model protein. The proposed immunosensor exhibits a wide linear detection range (LDR) from 1 fg mL⁻¹ to 100 ng mL⁻¹, and an ultralow limit of detection (LOD) of 0.1 fg mL⁻¹ (S/N=3). In addition, for the detection of clinical serum samples, it is well consistent with the data determined by the developed immunoassay and ELISA, indicating that the immunosensor provides a possible application for the detection of CEA in clinical diagnostics. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/23644143/Ionic_liquid_functionalized_graphene/Au_nanocomposites_and_its_application_for_electrochemical_immunosensor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(13)00261-3 DB - PRIME DP - Unbound Medicine ER -