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An efficient chemical analysis of phenolic acids and flavonoids in raw propolis by microwave-assisted extraction combined with high-performance liquid chromatography using the fused-core technology.
J Pharm Biomed Anal. 2013 Jul-Aug; 81-82:126-32.JP

Abstract

A closed-vessel microwave-assisted extraction (MAE) technique was optimized for the first time for the extraction of polyphenols from raw propolis. The results obtained by means of response surface experimental design methodology showed that the best global response was reached when the extraction temperature was set at 106 °C, the solvent composition close to EtOH-H2O 80:20 (v/v), with an extraction time of 15 min. In comparison with other techniques, such as maceration, heat reflux extraction (HRE) and ultrasound-assisted extraction (UAE), the extraction with MAE was improved by shorter extraction time and lower volume of solvent needed. The HPLC analyses of propolis extracts were carried out on a fused-core Ascentis Express C18 column (150 mm × 3.0 mm I.D., 2.7 μm), with a gradient mobile phase composed by 0.1% formic acid in water and acetonitrile. Detection was performed by DAD and MS. The method validation indicated that the correlation coefficients were >0.999; the limit of detection was in the range 0.5-0.8 μg/ml for phenolic acids and 1.2-3.0 μg/ml for flavonoids; the recovery range was 95.3-98.1% for phenolic acids and 94.1-101.3% for flavonoids; the intra- and inter-day %RSD values for retention times and peak areas were ≤ 0.3 and 2.2%, respectively. The quali- and quantitative analysis of polyphenols in Italian samples of raw propolis was performed with the validated method. Total phenolic acids ranged from 5.0 to 120.8 mg/g and total flavonoids from 2.5 to 168.0mg/g. The proposed MAE procedure and HPLC method can be considered reliable and useful tools for the comprehensive multi-component analysis of polyphenols in propolis extracts to be used in apitherapy.

Authors+Show Affiliations

Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 183, 41125 Modena, Italy. federica.pellati@unimore.itNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

23644907

Citation

Pellati, Federica, et al. "An Efficient Chemical Analysis of Phenolic Acids and Flavonoids in Raw Propolis By Microwave-assisted Extraction Combined With High-performance Liquid Chromatography Using the Fused-core Technology." Journal of Pharmaceutical and Biomedical Analysis, vol. 81-82, 2013, pp. 126-32.
Pellati F, Prencipe FP, Bertelli D, et al. An efficient chemical analysis of phenolic acids and flavonoids in raw propolis by microwave-assisted extraction combined with high-performance liquid chromatography using the fused-core technology. J Pharm Biomed Anal. 2013;81-82:126-32.
Pellati, F., Prencipe, F. P., Bertelli, D., & Benvenuti, S. (2013). An efficient chemical analysis of phenolic acids and flavonoids in raw propolis by microwave-assisted extraction combined with high-performance liquid chromatography using the fused-core technology. Journal of Pharmaceutical and Biomedical Analysis, 81-82, 126-32. https://doi.org/10.1016/j.jpba.2013.04.003
Pellati F, et al. An Efficient Chemical Analysis of Phenolic Acids and Flavonoids in Raw Propolis By Microwave-assisted Extraction Combined With High-performance Liquid Chromatography Using the Fused-core Technology. J Pharm Biomed Anal. 2013 Jul-Aug;81-82:126-32. PubMed PMID: 23644907.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An efficient chemical analysis of phenolic acids and flavonoids in raw propolis by microwave-assisted extraction combined with high-performance liquid chromatography using the fused-core technology. AU - Pellati,Federica, AU - Prencipe,Francesco Pio, AU - Bertelli,Davide, AU - Benvenuti,Stefania, Y1 - 2013/04/11/ PY - 2013/02/08/received PY - 2013/04/03/revised PY - 2013/04/05/accepted PY - 2013/5/7/entrez PY - 2013/5/7/pubmed PY - 2014/1/1/medline SP - 126 EP - 32 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 81-82 N2 - A closed-vessel microwave-assisted extraction (MAE) technique was optimized for the first time for the extraction of polyphenols from raw propolis. The results obtained by means of response surface experimental design methodology showed that the best global response was reached when the extraction temperature was set at 106 °C, the solvent composition close to EtOH-H2O 80:20 (v/v), with an extraction time of 15 min. In comparison with other techniques, such as maceration, heat reflux extraction (HRE) and ultrasound-assisted extraction (UAE), the extraction with MAE was improved by shorter extraction time and lower volume of solvent needed. The HPLC analyses of propolis extracts were carried out on a fused-core Ascentis Express C18 column (150 mm × 3.0 mm I.D., 2.7 μm), with a gradient mobile phase composed by 0.1% formic acid in water and acetonitrile. Detection was performed by DAD and MS. The method validation indicated that the correlation coefficients were >0.999; the limit of detection was in the range 0.5-0.8 μg/ml for phenolic acids and 1.2-3.0 μg/ml for flavonoids; the recovery range was 95.3-98.1% for phenolic acids and 94.1-101.3% for flavonoids; the intra- and inter-day %RSD values for retention times and peak areas were ≤ 0.3 and 2.2%, respectively. The quali- and quantitative analysis of polyphenols in Italian samples of raw propolis was performed with the validated method. Total phenolic acids ranged from 5.0 to 120.8 mg/g and total flavonoids from 2.5 to 168.0mg/g. The proposed MAE procedure and HPLC method can be considered reliable and useful tools for the comprehensive multi-component analysis of polyphenols in propolis extracts to be used in apitherapy. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/23644907/An_efficient_chemical_analysis_of_phenolic_acids_and_flavonoids_in_raw_propolis_by_microwave_assisted_extraction_combined_with_high_performance_liquid_chromatography_using_the_fused_core_technology_ DB - PRIME DP - Unbound Medicine ER -