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Separation of durum wheat proteins by ultrathin-layer isoelectric focusing: a new tool for the characterization and quantification of low molecular weight glutenins.
Electrophoresis. 1990 May; 11(5):392-9.E

Abstract

An isoelectric focusing method capable of resolving all groups of storage protein of the wheat seed, including the most basic low molecular weight glutenin (LMWG), was developed. Ultrathin polyacrylamide gels were used after drying and rehydration with 8 M urea, 50 mM DTE and 2.4% carrier ampholytes (pH 4-9). Densitometric scanning of the isoelectric focusing gels permitted a more accurate and specific quantitation of LMWG components among various cultivars than patterns based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The two main genetic types (i.e. 'gamma-42' and 'gamma-45') of durum wheats were separated on the basis of the proportion in LMWG in storage proteins, but no significant difference was found within these groups. Advantages of the system as regards reliability, high resolution, ability to abolish protein oxidation and preventing reaggregation of LMWG were also discussed.

Authors+Show Affiliations

Morel MH
Laboratoire de Technologie des Céréales I.N.R.A., Montpellier, France.
Autran JC
No affiliation info available

MeSH

DensitometryElectrophoresis, Gel, Two-DimensionalGlutensIsoelectric FocusingMolecular WeightSeedsTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2364926

Citation

Morel, M H., and J C. Autran. "Separation of Durum Wheat Proteins By Ultrathin-layer Isoelectric Focusing: a New Tool for the Characterization and Quantification of Low Molecular Weight Glutenins." Electrophoresis, vol. 11, no. 5, 1990, pp. 392-9.
Morel MH, Autran JC. Separation of durum wheat proteins by ultrathin-layer isoelectric focusing: a new tool for the characterization and quantification of low molecular weight glutenins. Electrophoresis. 1990;11(5):392-9.
Morel, M. H., & Autran, J. C. (1990). Separation of durum wheat proteins by ultrathin-layer isoelectric focusing: a new tool for the characterization and quantification of low molecular weight glutenins. Electrophoresis, 11(5), 392-9.
Morel MH, Autran JC. Separation of Durum Wheat Proteins By Ultrathin-layer Isoelectric Focusing: a New Tool for the Characterization and Quantification of Low Molecular Weight Glutenins. Electrophoresis. 1990;11(5):392-9. PubMed PMID: 2364926.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation of durum wheat proteins by ultrathin-layer isoelectric focusing: a new tool for the characterization and quantification of low molecular weight glutenins. AU - Morel,M H, AU - Autran,J C, PY - 1990/5/1/pubmed PY - 1990/5/1/medline PY - 1990/5/1/entrez SP - 392 EP - 9 JF - Electrophoresis JO - Electrophoresis VL - 11 IS - 5 N2 - An isoelectric focusing method capable of resolving all groups of storage protein of the wheat seed, including the most basic low molecular weight glutenin (LMWG), was developed. Ultrathin polyacrylamide gels were used after drying and rehydration with 8 M urea, 50 mM DTE and 2.4% carrier ampholytes (pH 4-9). Densitometric scanning of the isoelectric focusing gels permitted a more accurate and specific quantitation of LMWG components among various cultivars than patterns based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The two main genetic types (i.e. 'gamma-42' and 'gamma-45') of durum wheats were separated on the basis of the proportion in LMWG in storage proteins, but no significant difference was found within these groups. Advantages of the system as regards reliability, high resolution, ability to abolish protein oxidation and preventing reaggregation of LMWG were also discussed. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/2364926/Separation_of_durum_wheat_proteins_by_ultrathin_layer_isoelectric_focusing:_a_new_tool_for_the_characterization_and_quantification_of_low_molecular_weight_glutenins_ DB - PRIME DP - Unbound Medicine ER -