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[Effect of electroacupuncture on expression of phosphorylated P 38 MAPK and IL-1beta in frontal lobe and hippocampus in rats with Alzheimer's disease].
Zhen Ci Yan Jiu. 2013 Feb; 38(1):35-9.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) intervention on expression of phosphorylated mitogen activated protein kinase-P 38 (p-P 38 MAPK) protein and Interleukin 1beta (IL-1beta)mRNA in the frontal lobe and hippocampus in Alzheimer's disease (AD) rats so as to explore its mechanisms underlying improvement of AD in clinic.

METHODS

Thirty-two SD rats were equally and randomly divided into normal control (normal), sham-operation (sham), model and EA groups. AD model was established by microinjection of Abeta(1-40) (10 microg/microL, 1 microL) into bilateral Meynert nucleus (AP: 1. 4 mm, L: 3 mm, H: 7 mm) and validated by water maze tests. Rats of the sham group were treated by microinjection of the same dose of normal saline into the bilateral Meynert Nucleus. EA (1 mA, 2 Hz) was applied to bilateral "Baihui" (GV 20), "Taixi" (KI 3) and "Zusanli" (ST 36) for 15 min, once daily for 12 sessions. Expression levels of p-P 38 MAPK protein and IL-1beta mRNA in the hippocampus and frontal lobe tissues were detected by Western blot and RT-PCR, respectively.

RESULTS

In comparison with the normal group, the expression levels of p-P 38 MAPK protein and IL-1beta mRNA in the hippocampus and frontal lobe tissues were upregulated significantly in the model group (P < 0.01). After 12 sessions of EA intervention, the expression levels of both p-P 38 MAPK protein and IL-1beta mRNA were down-regulated significantly (P < 0.01, P < 0.05) in spite of being still higher than those of the normal group. No significant differences were found between the normal and sham groups in the expression levels of both p-P 38 MAPK protein and IL-1beta mRNA (P > 0.05).

CONCLUSION

EA intervention can reduce the over expression of both p-P 38 MAPK protein and IL-1beta mRNA in the hippocampus and frontal cortex in AD rats, suggesting an improvement of AD after EA intervention by restraining the inflammatory reaction.

Authors+Show Affiliations

Fang JQ
Zhejiang University of Chinese Medicine, Hangzhou 310005, China.
Zhu SX
No affiliation info available
Zhang Y
No affiliation info available
Wang F
No affiliation info available
Zhu QY
No affiliation info available

MeSH

Acupuncture PointsAlzheimer DiseaseAnimalsDisease Models, AnimalElectroacupunctureFemaleFrontal LobeHippocampusHumansInterleukin-1betaMalePhosphorylationRatsRats, Sprague-Dawleyp38 Mitogen-Activated Protein Kinases

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23650798

Citation

Fang, Jian-Qiao, et al. "[Effect of Electroacupuncture On Expression of Phosphorylated P 38 MAPK and IL-1beta in Frontal Lobe and Hippocampus in Rats With Alzheimer's Disease]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 38, no. 1, 2013, pp. 35-9.
Fang JQ, Zhu SX, Zhang Y, et al. [Effect of electroacupuncture on expression of phosphorylated P 38 MAPK and IL-1beta in frontal lobe and hippocampus in rats with Alzheimer's disease]. Zhen Ci Yan Jiu. 2013;38(1):35-9.
Fang, J. Q., Zhu, S. X., Zhang, Y., Wang, F., & Zhu, Q. Y. (2013). [Effect of electroacupuncture on expression of phosphorylated P 38 MAPK and IL-1beta in frontal lobe and hippocampus in rats with Alzheimer's disease]. Zhen Ci Yan Jiu = Acupuncture Research, 38(1), 35-9.
Fang JQ, et al. [Effect of Electroacupuncture On Expression of Phosphorylated P 38 MAPK and IL-1beta in Frontal Lobe and Hippocampus in Rats With Alzheimer's Disease]. Zhen Ci Yan Jiu. 2013;38(1):35-9. PubMed PMID: 23650798.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture on expression of phosphorylated P 38 MAPK and IL-1beta in frontal lobe and hippocampus in rats with Alzheimer's disease]. AU - Fang,Jian-Qiao, AU - Zhu,Shu-Xiu, AU - Zhang,Ying, AU - Wang,Fang, AU - Zhu,Qing-Yan, PY - 2013/5/9/entrez PY - 2013/5/9/pubmed PY - 2013/7/3/medline SP - 35 EP - 9 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 38 IS - 1 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) intervention on expression of phosphorylated mitogen activated protein kinase-P 38 (p-P 38 MAPK) protein and Interleukin 1beta (IL-1beta)mRNA in the frontal lobe and hippocampus in Alzheimer's disease (AD) rats so as to explore its mechanisms underlying improvement of AD in clinic. METHODS: Thirty-two SD rats were equally and randomly divided into normal control (normal), sham-operation (sham), model and EA groups. AD model was established by microinjection of Abeta(1-40) (10 microg/microL, 1 microL) into bilateral Meynert nucleus (AP: 1. 4 mm, L: 3 mm, H: 7 mm) and validated by water maze tests. Rats of the sham group were treated by microinjection of the same dose of normal saline into the bilateral Meynert Nucleus. EA (1 mA, 2 Hz) was applied to bilateral "Baihui" (GV 20), "Taixi" (KI 3) and "Zusanli" (ST 36) for 15 min, once daily for 12 sessions. Expression levels of p-P 38 MAPK protein and IL-1beta mRNA in the hippocampus and frontal lobe tissues were detected by Western blot and RT-PCR, respectively. RESULTS: In comparison with the normal group, the expression levels of p-P 38 MAPK protein and IL-1beta mRNA in the hippocampus and frontal lobe tissues were upregulated significantly in the model group (P < 0.01). After 12 sessions of EA intervention, the expression levels of both p-P 38 MAPK protein and IL-1beta mRNA were down-regulated significantly (P < 0.01, P < 0.05) in spite of being still higher than those of the normal group. No significant differences were found between the normal and sham groups in the expression levels of both p-P 38 MAPK protein and IL-1beta mRNA (P > 0.05). CONCLUSION: EA intervention can reduce the over expression of both p-P 38 MAPK protein and IL-1beta mRNA in the hippocampus and frontal cortex in AD rats, suggesting an improvement of AD after EA intervention by restraining the inflammatory reaction. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/23650798/[Effect_of_electroacupuncture_on_expression_of_phosphorylated_P_38_MAPK_and_IL_1beta_in_frontal_lobe_and_hippocampus_in_rats_with_Alzheimer's_disease]_ L2 - https://medlineplus.gov/alzheimersdisease.html DB - PRIME DP - Unbound Medicine ER -

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