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Protein tyrosine phosphatase encoded in Cotesia plutellae bracovirus suppresses a larva-to-pupa metamorphosis of the diamondback moth, Plutella xylostella.

Abstract

Parasitization by an endoparasitoid wasp, Cotesia plutellae, inhibits a larva-to-pupa metamorphosis of the diamondback moth, Plutella xylostella. This study tested an inhibitory effect of C. plutellae bracovirus (CpBV) on the metamorphosis of P. xylostella. Parasitized P. xylostella exhibited significantly reduced prothoracic gland (PTG) development at the last instar compared to nonparasitized larvae. Expression of the ecdysone receptor (EcR) was markedly suppressed during the last instar larvae parasitized by C. plutellae. By contrast, expression of the insulin receptor (InR) significantly increased in the parasitized larvae. Microinjection of CpBV significantly inhibited the larva-to-pupa metamorphosis of nonparasitized larvae in a dose-dependent manner. Injection of CpBV also inhibited the expression of the EcR and increased the expression of the InR. Individual CpBV segments were transiently expressed in its encoded genes in nonparasitized larvae and screened to determine antimetamorphic viral gene(s). Out of 21 CpBV segments, two viral segments (CpBV-S22 and CpBV-S27) were proved to inhibit larva-to-pupa metamorphosis by transient expression assay. RNA interference of each gene encoded in the viral segments was applied to determine antimetamorphic gene(s). Protein tyrosine phosphatase, early expressed gene, and four hypothetical genes were selected to be associated with the antimetamorphic activity of CpBV. These results suggest that antimetamorphosis of P. xylostella parasitized by C. plutellae is induced by inhibiting PTG development and subsequent ecdysteroid signaling with viral factors of CpBV.

Authors+Show Affiliations

Department of Bioresource Sciences, Andong National University, Andong 760-749, Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23651929

Citation

Kim, Jiwan, et al. "Protein Tyrosine Phosphatase Encoded in Cotesia Plutellae Bracovirus Suppresses a Larva-to-pupa Metamorphosis of the Diamondback Moth, Plutella Xylostella." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 166, no. 1, 2013, pp. 60-9.
Kim J, Hepat R, Lee D, et al. Protein tyrosine phosphatase encoded in Cotesia plutellae bracovirus suppresses a larva-to-pupa metamorphosis of the diamondback moth, Plutella xylostella. Comp Biochem Physiol A Mol Integr Physiol. 2013;166(1):60-9.
Kim, J., Hepat, R., Lee, D., & Kim, Y. (2013). Protein tyrosine phosphatase encoded in Cotesia plutellae bracovirus suppresses a larva-to-pupa metamorphosis of the diamondback moth, Plutella xylostella. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 166(1), 60-9. https://doi.org/10.1016/j.cbpa.2013.04.025
Kim J, et al. Protein Tyrosine Phosphatase Encoded in Cotesia Plutellae Bracovirus Suppresses a Larva-to-pupa Metamorphosis of the Diamondback Moth, Plutella Xylostella. Comp Biochem Physiol A Mol Integr Physiol. 2013;166(1):60-9. PubMed PMID: 23651929.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein tyrosine phosphatase encoded in Cotesia plutellae bracovirus suppresses a larva-to-pupa metamorphosis of the diamondback moth, Plutella xylostella. AU - Kim,Jiwan, AU - Hepat,Rahul, AU - Lee,Daeweon, AU - Kim,Yonggyun, Y1 - 2013/05/04/ PY - 2013/03/18/received PY - 2013/04/29/revised PY - 2013/04/29/accepted PY - 2013/5/9/entrez PY - 2013/5/9/pubmed PY - 2014/1/24/medline KW - Cotesia plutellae KW - Ecdysone KW - Metamorphosis KW - Plutella xylostella KW - Polydnavirus SP - 60 EP - 9 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp Biochem Physiol A Mol Integr Physiol VL - 166 IS - 1 N2 - Parasitization by an endoparasitoid wasp, Cotesia plutellae, inhibits a larva-to-pupa metamorphosis of the diamondback moth, Plutella xylostella. This study tested an inhibitory effect of C. plutellae bracovirus (CpBV) on the metamorphosis of P. xylostella. Parasitized P. xylostella exhibited significantly reduced prothoracic gland (PTG) development at the last instar compared to nonparasitized larvae. Expression of the ecdysone receptor (EcR) was markedly suppressed during the last instar larvae parasitized by C. plutellae. By contrast, expression of the insulin receptor (InR) significantly increased in the parasitized larvae. Microinjection of CpBV significantly inhibited the larva-to-pupa metamorphosis of nonparasitized larvae in a dose-dependent manner. Injection of CpBV also inhibited the expression of the EcR and increased the expression of the InR. Individual CpBV segments were transiently expressed in its encoded genes in nonparasitized larvae and screened to determine antimetamorphic viral gene(s). Out of 21 CpBV segments, two viral segments (CpBV-S22 and CpBV-S27) were proved to inhibit larva-to-pupa metamorphosis by transient expression assay. RNA interference of each gene encoded in the viral segments was applied to determine antimetamorphic gene(s). Protein tyrosine phosphatase, early expressed gene, and four hypothetical genes were selected to be associated with the antimetamorphic activity of CpBV. These results suggest that antimetamorphosis of P. xylostella parasitized by C. plutellae is induced by inhibiting PTG development and subsequent ecdysteroid signaling with viral factors of CpBV. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/23651929/Protein_tyrosine_phosphatase_encoded_in_Cotesia_plutellae_bracovirus_suppresses_a_larva_to_pupa_metamorphosis_of_the_diamondback_moth_Plutella_xylostella_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(13)00118-9 DB - PRIME DP - Unbound Medicine ER -