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Association of a DNA virus with grapevines affected by red blotch disease in California.
Phytopathology. 2013 Oct; 103(10):1069-76.P

Abstract

In the Napa Valley of California, vineyards of 'Cabernet Franc' (CF) clone 214, 'Cabernet Sauvignon' clone 337, and 'Zinfandel' clone 1A (Z1A) with grapevines exhibiting foliar symptoms of red blotches, marginal reddening, and red veins that were accompanied by reduced sugar accumulation in fruit at harvest were initially suspected to be infected with leafroll-associated viruses. However, reverse-transcription polymerase chain reaction (PCR) tests were negative for all known leafroll-associated viruses, with the exception of Grapevine leafroll-associated virus 2 in Z1A. Metagenomic analysis of cDNA libraries obtained from double-stranded RNA enriched nucleic acid (NA) preparations from bark scrapings of dormant canes on an Illumina platform revealed sequences having a distant relationship with members of the family Geminiviridae. Sequencing of products obtained by PCR assays using overlapping primers and rolling circle amplification (RCA) confirmed the presence of a single circular genome of 3,206 nucleotides which was nearly identical to the genome of a recently reported Grapevine cabernet franc-associated virus found in declining grapevines in New York. We propose to call this virus "Grapevine red blotch-associated virus" (GRBaV) to describe its association with grapevine red blotch disease. Primers specific to GRBaV amplified a product of expected size (557 bp) from NA preparations obtained from petioles of several diseased source vines. Chip bud inoculations successfully transmitted GRBaV to test plants of CF, as confirmed by PCR analysis. This is the first report of a DNA virus associated with red blotch disease of grapevines in California.

Links

Publisher Full Text
apsjournals.apsnet.org
apsjournals.apsnet.org

Authors

Al Rwahnih M
No affiliation info available
Dave A
No affiliation info available
Anderson MM
No affiliation info available
Rowhani A
No affiliation info available
Uyemoto JK
No affiliation info available
Sudarshana MR
No affiliation info available

MeSH

ClosterovirusGeminiviridaePlant DiseasesRNA, ViralReverse Transcriptase Polymerase Chain ReactionSequence Analysis, DNAVitis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23656312

Citation

Al Rwahnih, Maher, et al. "Association of a DNA Virus With Grapevines Affected By Red Blotch Disease in California." Phytopathology, vol. 103, no. 10, 2013, pp. 1069-76.
Al Rwahnih M, Dave A, Anderson MM, et al. Association of a DNA virus with grapevines affected by red blotch disease in California. Phytopathology. 2013;103(10):1069-76.
Al Rwahnih, M., Dave, A., Anderson, M. M., Rowhani, A., Uyemoto, J. K., & Sudarshana, M. R. (2013). Association of a DNA virus with grapevines affected by red blotch disease in California. Phytopathology, 103(10), 1069-76. https://doi.org/10.1094/PHYTO-10-12-0253-R
Al Rwahnih M, et al. Association of a DNA Virus With Grapevines Affected By Red Blotch Disease in California. Phytopathology. 2013;103(10):1069-76. PubMed PMID: 23656312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of a DNA virus with grapevines affected by red blotch disease in California. AU - Al Rwahnih,Maher, AU - Dave,Ashita, AU - Anderson,Michael M, AU - Rowhani,Adib, AU - Uyemoto,Jerry K, AU - Sudarshana,Mysore R, PY - 2013/5/10/entrez PY - 2013/5/10/pubmed PY - 2016/4/24/medline SP - 1069 EP - 76 JF - Phytopathology JO - Phytopathology VL - 103 IS - 10 N2 - In the Napa Valley of California, vineyards of 'Cabernet Franc' (CF) clone 214, 'Cabernet Sauvignon' clone 337, and 'Zinfandel' clone 1A (Z1A) with grapevines exhibiting foliar symptoms of red blotches, marginal reddening, and red veins that were accompanied by reduced sugar accumulation in fruit at harvest were initially suspected to be infected with leafroll-associated viruses. However, reverse-transcription polymerase chain reaction (PCR) tests were negative for all known leafroll-associated viruses, with the exception of Grapevine leafroll-associated virus 2 in Z1A. Metagenomic analysis of cDNA libraries obtained from double-stranded RNA enriched nucleic acid (NA) preparations from bark scrapings of dormant canes on an Illumina platform revealed sequences having a distant relationship with members of the family Geminiviridae. Sequencing of products obtained by PCR assays using overlapping primers and rolling circle amplification (RCA) confirmed the presence of a single circular genome of 3,206 nucleotides which was nearly identical to the genome of a recently reported Grapevine cabernet franc-associated virus found in declining grapevines in New York. We propose to call this virus "Grapevine red blotch-associated virus" (GRBaV) to describe its association with grapevine red blotch disease. Primers specific to GRBaV amplified a product of expected size (557 bp) from NA preparations obtained from petioles of several diseased source vines. Chip bud inoculations successfully transmitted GRBaV to test plants of CF, as confirmed by PCR analysis. This is the first report of a DNA virus associated with red blotch disease of grapevines in California. SN - 0031-949X UR - https://www.unboundmedicine.com/medline/citation/23656312/Association_of_a_DNA_virus_with_grapevines_affected_by_red_blotch_disease_in_California_ L2 - https://apsjournals.apsnet.org/doi/10.1094/PHYTO-10-12-0253-R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -