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Determination of the major phenolic compounds in pomegranate juices by HPLC−DAD−ESI-MS.
J Agric Food Chem. 2013 Jun 05; 61(22):5328-37.JA

Abstract

Traditionally, pomegranate (Punica granatum L.) has been consumed as fresh fruit or as pomegranate juice. In this study, the main phenolic compounds of 12 pomegranate varieties and 5 pomegranate clones were determined by HPLC−DAD−ESI-MS. Two chromatographic methods with a fused-core C18 column and a classical HPLC system were developed. Thirteen anthocyanins and fourteen other phenolic compounds were determined in the pomegranate juices. As far as we are concerned, a new flavonol-glycoside, phellatin or its isomer amurensin, has been tentatively identified for the first time in pomegranate juices. Total phenolic content ranged from 580.8 to 2551.3 mg/L of pomegranate juice. Anthocyanins varied between 20 to 82% of total phenolic content. Flavonoids were 1.6-23.6% of total phenolic compounds, while phenolic acids and ellagitannins were in the range 16.4-65.8%. The five clones reported a phenolic content comparable with that of the other pomegranate samples.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Gómez-Caravaca AM
Department of Analytical Chemistry, University of Granada, c/Fuentenueva s/n, 18071, Granada, Spain.
Verardo V
No affiliation info available
Toselli M
No affiliation info available
Segura-Carretero A
No affiliation info available
Fernández-Gutiérrez A
No affiliation info available
Caboni MF
No affiliation info available

MeSH

AnthocyaninsAntioxidantsBeveragesChromatography, High Pressure LiquidCloning, OrganismElectrochemical TechniquesFlavonoidsFruitFunctional FoodHydrolyzable TanninsLythraceaeMediterranean RegionMolecular StructurePhenolsSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23656584

Citation

Gómez-Caravaca, Ana María, et al. "Determination of the Major Phenolic Compounds in Pomegranate Juices By HPLC−DAD−ESI-MS." Journal of Agricultural and Food Chemistry, vol. 61, no. 22, 2013, pp. 5328-37.
Gómez-Caravaca AM, Verardo V, Toselli M, et al. Determination of the major phenolic compounds in pomegranate juices by HPLC−DAD−ESI-MS. J Agric Food Chem. 2013;61(22):5328-37.
Gómez-Caravaca, A. M., Verardo, V., Toselli, M., Segura-Carretero, A., Fernández-Gutiérrez, A., & Caboni, M. F. (2013). Determination of the major phenolic compounds in pomegranate juices by HPLC−DAD−ESI-MS. Journal of Agricultural and Food Chemistry, 61(22), 5328-37. https://doi.org/10.1021/jf400684n
Gómez-Caravaca AM, et al. Determination of the Major Phenolic Compounds in Pomegranate Juices By HPLC−DAD−ESI-MS. J Agric Food Chem. 2013 Jun 5;61(22):5328-37. PubMed PMID: 23656584.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of the major phenolic compounds in pomegranate juices by HPLC−DAD−ESI-MS. AU - Gómez-Caravaca,Ana María, AU - Verardo,Vito, AU - Toselli,Moreno, AU - Segura-Carretero,Antonio, AU - Fernández-Gutiérrez,Alberto, AU - Caboni,Maria Fiorenza, Y1 - 2013/05/24/ PY - 2013/5/10/entrez PY - 2013/5/10/pubmed PY - 2014/7/16/medline SP - 5328 EP - 37 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 61 IS - 22 N2 - Traditionally, pomegranate (Punica granatum L.) has been consumed as fresh fruit or as pomegranate juice. In this study, the main phenolic compounds of 12 pomegranate varieties and 5 pomegranate clones were determined by HPLC−DAD−ESI-MS. Two chromatographic methods with a fused-core C18 column and a classical HPLC system were developed. Thirteen anthocyanins and fourteen other phenolic compounds were determined in the pomegranate juices. As far as we are concerned, a new flavonol-glycoside, phellatin or its isomer amurensin, has been tentatively identified for the first time in pomegranate juices. Total phenolic content ranged from 580.8 to 2551.3 mg/L of pomegranate juice. Anthocyanins varied between 20 to 82% of total phenolic content. Flavonoids were 1.6-23.6% of total phenolic compounds, while phenolic acids and ellagitannins were in the range 16.4-65.8%. The five clones reported a phenolic content comparable with that of the other pomegranate samples. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/23656584/Determination_of_the_major_phenolic_compounds_in_pomegranate_juices_by_HPLC−DAD−ESI_MS_ L2 - https://doi.org/10.1021/jf400684n DB - PRIME DP - Unbound Medicine ER -