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Vitamin B12-binding proteins in human gastric mucosa. General pattern and demonstration of intrinsic factor isoproteins typical of mucosa.
Scand J Clin Lab Invest. 1975 Jan; 35(1):5-11.SJ

Abstract

Extracts of human stomach mucosa were shown to contain immunoreactive intrinsic factor and small amounts of R protein and transcobalamin II. In gel filtration the mucosal vitamin B12-intrinsic factor complex behaved as though its molecular weight were smaller than the corresponding complex in gastric juice. In isoelectric focusing both the gastric juice and mucosal vitamin B12-intrinsic factor complexes resolved into numerous isoproteins. These findings were verified by DEAE-cellulose chromatography. The mucosal intrinsic factor isoprotein pattern contained, in addition to the isoproteins found in gastric juice, components with higher isoelectric points -- that is, pH 5.73-6.64.

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Authors

Marcoullis G
No affiliation info available
Gräsbeck R
No affiliation info available

MeSH

Blood ProteinsCentrifugation, Density GradientChromatography, DEAE-CelluloseGastric JuiceGastric MucosaHumansHydrogen-Ion ConcentrationIntrinsic FactorIsoelectric FocusingProteinsTranscobalaminsVitamin B 12

Pub Type(s)

Journal Article

Language

eng

PubMed ID

236593

Citation

Marcoullis, G, and R Gräsbeck. "Vitamin B12-binding Proteins in Human Gastric Mucosa. General Pattern and Demonstration of Intrinsic Factor Isoproteins Typical of Mucosa." Scandinavian Journal of Clinical and Laboratory Investigation, vol. 35, no. 1, 1975, pp. 5-11.
Marcoullis G, Gräsbeck R. Vitamin B12-binding proteins in human gastric mucosa. General pattern and demonstration of intrinsic factor isoproteins typical of mucosa. Scand J Clin Lab Invest. 1975;35(1):5-11.
Marcoullis, G., & Gräsbeck, R. (1975). Vitamin B12-binding proteins in human gastric mucosa. General pattern and demonstration of intrinsic factor isoproteins typical of mucosa. Scandinavian Journal of Clinical and Laboratory Investigation, 35(1), 5-11.
Marcoullis G, Gräsbeck R. Vitamin B12-binding Proteins in Human Gastric Mucosa. General Pattern and Demonstration of Intrinsic Factor Isoproteins Typical of Mucosa. Scand J Clin Lab Invest. 1975;35(1):5-11. PubMed PMID: 236593.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vitamin B12-binding proteins in human gastric mucosa. General pattern and demonstration of intrinsic factor isoproteins typical of mucosa. AU - Marcoullis,G, AU - Gräsbeck,R, PY - 1975/1/1/pubmed PY - 1975/1/1/medline PY - 1975/1/1/entrez SP - 5 EP - 11 JF - Scandinavian journal of clinical and laboratory investigation JO - Scand. J. Clin. Lab. Invest. VL - 35 IS - 1 N2 - Extracts of human stomach mucosa were shown to contain immunoreactive intrinsic factor and small amounts of R protein and transcobalamin II. In gel filtration the mucosal vitamin B12-intrinsic factor complex behaved as though its molecular weight were smaller than the corresponding complex in gastric juice. In isoelectric focusing both the gastric juice and mucosal vitamin B12-intrinsic factor complexes resolved into numerous isoproteins. These findings were verified by DEAE-cellulose chromatography. The mucosal intrinsic factor isoprotein pattern contained, in addition to the isoproteins found in gastric juice, components with higher isoelectric points -- that is, pH 5.73-6.64. SN - 0036-5513 UR - https://www.unboundmedicine.com/medline/citation/236593/Vitamin_B12_binding_proteins_in_human_gastric_mucosa__General_pattern_and_demonstration_of_intrinsic_factor_isoproteins_typical_of_mucosa_ L2 - http://www.tandfonline.com/doi/full/10.1080/00365517509067998 DB - PRIME DP - Unbound Medicine ER -