Vitamin B12-binding proteins in human gastric mucosa. General pattern and demonstration of intrinsic factor isoproteins typical of mucosa.Scand J Clin Lab Invest. 1975 Jan; 35(1):5-11.SJ
Abstract
Extracts of human stomach mucosa were shown to contain immunoreactive intrinsic factor and small amounts of R protein and transcobalamin II. In gel filtration the mucosal vitamin B12-intrinsic factor complex behaved as though its molecular weight were smaller than the corresponding complex in gastric juice. In isoelectric focusing both the gastric juice and mucosal vitamin B12-intrinsic factor complexes resolved into numerous isoproteins. These findings were verified by DEAE-cellulose chromatography. The mucosal intrinsic factor isoprotein pattern contained, in addition to the isoproteins found in gastric juice, components with higher isoelectric points -- that is, pH 5.73-6.64.
Links
MeSH
Pub Type(s)
Journal Article
Language
eng
PubMed ID
236593
Citation
Marcoullis, G, and R Gräsbeck. "Vitamin B12-binding Proteins in Human Gastric Mucosa. General Pattern and Demonstration of Intrinsic Factor Isoproteins Typical of Mucosa." Scandinavian Journal of Clinical and Laboratory Investigation, vol. 35, no. 1, 1975, pp. 5-11.
Marcoullis G, Gräsbeck R. Vitamin B12-binding proteins in human gastric mucosa. General pattern and demonstration of intrinsic factor isoproteins typical of mucosa. Scand J Clin Lab Invest. 1975;35(1):5-11.
Marcoullis, G., & Gräsbeck, R. (1975). Vitamin B12-binding proteins in human gastric mucosa. General pattern and demonstration of intrinsic factor isoproteins typical of mucosa. Scandinavian Journal of Clinical and Laboratory Investigation, 35(1), 5-11.
Marcoullis G, Gräsbeck R. Vitamin B12-binding Proteins in Human Gastric Mucosa. General Pattern and Demonstration of Intrinsic Factor Isoproteins Typical of Mucosa. Scand J Clin Lab Invest. 1975;35(1):5-11. PubMed PMID: 236593.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Vitamin B12-binding proteins in human gastric mucosa. General pattern and demonstration of intrinsic factor isoproteins typical of mucosa.
AU - Marcoullis,G,
AU - Gräsbeck,R,
PY - 1975/1/1/pubmed
PY - 1975/1/1/medline
PY - 1975/1/1/entrez
SP - 5
EP - 11
JF - Scandinavian journal of clinical and laboratory investigation
JO - Scand J Clin Lab Invest
VL - 35
IS - 1
N2 - Extracts of human stomach mucosa were shown to contain immunoreactive intrinsic factor and small amounts of R protein and transcobalamin II. In gel filtration the mucosal vitamin B12-intrinsic factor complex behaved as though its molecular weight were smaller than the corresponding complex in gastric juice. In isoelectric focusing both the gastric juice and mucosal vitamin B12-intrinsic factor complexes resolved into numerous isoproteins. These findings were verified by DEAE-cellulose chromatography. The mucosal intrinsic factor isoprotein pattern contained, in addition to the isoproteins found in gastric juice, components with higher isoelectric points -- that is, pH 5.73-6.64.
SN - 0036-5513
UR - https://www.unboundmedicine.com/medline/citation/236593/Vitamin_B12_binding_proteins_in_human_gastric_mucosa__General_pattern_and_demonstration_of_intrinsic_factor_isoproteins_typical_of_mucosa_
L2 - https://www.tandfonline.com/doi/full/10.1080/00365517509067998
DB - PRIME
DP - Unbound Medicine
ER -