Tags

Type your tag names separated by a space and hit enter

Genomic and chromosomal distribution patterns of various repeated DNA sequences in wheat revealed by a fluorescence in situ hybridization procedure.
Genome. 2013 Mar; 56(3):131-7.G

Abstract

Wheat (Triticum aestivum L.) is an allohexaploid, in which each of the three genomes has a high 1C content. This indicates the presence of multiple tandemly repeated sequences, which should be detectable using in situ hybridization. Some repeats have already been described, but others remain to be recognized. To discover others, 2000 plasmid wheat clones were examined for signal presence after fluorescence in situ hybridization and microscopic signal observation. Among them, 47 clones produced strong discrete signals on wheat chromosomes. Two of the newly identified clones (pTa-535 and pTa-713) were determined to have especially valuable sequences for chromosome identification. In combination with pTa-86 (the pSc119 homologous sequence), these probes enable unambiguous discrimination of all wheat chromosomes including orientation. Four newly identified sequences (pTa-465, pTa-k566, pTa-s120, and pTa-s126) were useful in that they produced discrete signals on various wheat chromosome arms. Two other clones (pTa-k288 and pTa-k229) produced GISH-like (genomic in situ hybridization) signals because they allowed the A, B, and D genomes to be distinguished simultaneously. In addition, centromere, centromere-related, and ribosomal DNA clones were identified. Also described are improvements on slide preparation and reprobing procedures. To enhance discrete signal detection, a new direct fluorescent-labeling procedure, namely the VentR (exo-) terminal extension method, was employed.

Links

Publisher Full Text
Aggregator Full Text

Authors+Show Affiliations

Komuro S
Laboratory of Plant Breeding, Faculty of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Shimogamo Hangi-cho, Sakyo-ku, Kyoto-shi, Kyoto-fu 606-0823, Japan.
Endo R
No affiliation info available
Shikata K
No affiliation info available
Kato A
No affiliation info available

MeSH

Chromosome MappingChromosomes, PlantCloning, MolecularGene LibraryGenes, PlantGenome, PlantGenomicsIn Situ Hybridization, FluorescenceMolecular Sequence DataRepetitive Sequences, Nucleic AcidTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23659696

Citation

Komuro, Shirabe, et al. "Genomic and Chromosomal Distribution Patterns of Various Repeated DNA Sequences in Wheat Revealed By a Fluorescence in Situ Hybridization Procedure." Genome, vol. 56, no. 3, 2013, pp. 131-7.
Komuro S, Endo R, Shikata K, et al. Genomic and chromosomal distribution patterns of various repeated DNA sequences in wheat revealed by a fluorescence in situ hybridization procedure. Genome. 2013;56(3):131-7.
Komuro, S., Endo, R., Shikata, K., & Kato, A. (2013). Genomic and chromosomal distribution patterns of various repeated DNA sequences in wheat revealed by a fluorescence in situ hybridization procedure. Genome, 56(3), 131-7. https://doi.org/10.1139/gen-2013-0003
Komuro S, et al. Genomic and Chromosomal Distribution Patterns of Various Repeated DNA Sequences in Wheat Revealed By a Fluorescence in Situ Hybridization Procedure. Genome. 2013;56(3):131-7. PubMed PMID: 23659696.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic and chromosomal distribution patterns of various repeated DNA sequences in wheat revealed by a fluorescence in situ hybridization procedure. AU - Komuro,Shirabe, AU - Endo,Ryota, AU - Shikata,Kaori, AU - Kato,Akio, Y1 - 2013/02/21/ PY - 2013/5/11/entrez PY - 2013/5/11/pubmed PY - 2013/10/23/medline SP - 131 EP - 7 JF - Genome JO - Genome VL - 56 IS - 3 N2 - Wheat (Triticum aestivum L.) is an allohexaploid, in which each of the three genomes has a high 1C content. This indicates the presence of multiple tandemly repeated sequences, which should be detectable using in situ hybridization. Some repeats have already been described, but others remain to be recognized. To discover others, 2000 plasmid wheat clones were examined for signal presence after fluorescence in situ hybridization and microscopic signal observation. Among them, 47 clones produced strong discrete signals on wheat chromosomes. Two of the newly identified clones (pTa-535 and pTa-713) were determined to have especially valuable sequences for chromosome identification. In combination with pTa-86 (the pSc119 homologous sequence), these probes enable unambiguous discrimination of all wheat chromosomes including orientation. Four newly identified sequences (pTa-465, pTa-k566, pTa-s120, and pTa-s126) were useful in that they produced discrete signals on various wheat chromosome arms. Two other clones (pTa-k288 and pTa-k229) produced GISH-like (genomic in situ hybridization) signals because they allowed the A, B, and D genomes to be distinguished simultaneously. In addition, centromere, centromere-related, and ribosomal DNA clones were identified. Also described are improvements on slide preparation and reprobing procedures. To enhance discrete signal detection, a new direct fluorescent-labeling procedure, namely the VentR (exo-) terminal extension method, was employed. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/23659696/Genomic_and_chromosomal_distribution_patterns_of_various_repeated_DNA_sequences_in_wheat_revealed_by_a_fluorescence_in_situ_hybridization_procedure_ L2 - https://cdnsciencepub.com/doi/10.1139/gen-2013-0003?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -