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Nigella sativa seed extract: 1. Enhancement of sheep macrophage immune functions in vitro.
Res Vet Sci. 2013 Oct; 95(2):437-43.RV

Abstract

Nigella sativa (N. sativa) seed, Black cumin, immunomodulatory activity has been investigated in human and mice. Little is known about the immunomodulatory effect of Nigella sativa (N. sativa) seed extract on animals' immune cells, specifically, antigen presenting cells such as macrophages. This study focused on the immunomodulatory effect of N. sativa seed extract on sheep macrophage functions in vitro. Sheep peripheral blood monocytes were isolated and derived to macrophages (MDM). The MDM were cultured with N. sativa seed extract and their morphological changes, phagocytic activity, nitric oxide production, and microbicidal activity were investigated. Marked morphological changes were observed in MDM cultured with N. sativa seed extract including cell size enlargement; increase in both cytoplasmic space and cytoplasmic granules. Significant increases in phagocytic activity to Candida albicans yeast and in number of yeast engulfed per individual MDM were observed in cells cultured with seed extract. MDM capacity to produce nitric oxide was higher in the culture media of the seed extract-cultured cells compared to the control. Interestingly, prominent enhancement in MDM microbicidal activity to yeast or bacteria was observed in MDM cultured with N. sativa seed extract confirming the potent immunostimulatory effect of the extract. From this study, it could be concluded that N. sativa seed extract can enhance macrophages' important innate immune functions that could control infectious diseases and regulate adaptive immunity.

Authors+Show Affiliations

Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia Governorate, Egypt. gelmowalid@yahoo.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23664216

Citation

Elmowalid, Gamal, et al. "Nigella Sativa Seed Extract: 1. Enhancement of Sheep Macrophage Immune Functions in Vitro." Research in Veterinary Science, vol. 95, no. 2, 2013, pp. 437-43.
Elmowalid G, Amar AM, Ahmad AA. Nigella sativa seed extract: 1. Enhancement of sheep macrophage immune functions in vitro. Res Vet Sci. 2013;95(2):437-43.
Elmowalid, G., Amar, A. M., & Ahmad, A. A. (2013). Nigella sativa seed extract: 1. Enhancement of sheep macrophage immune functions in vitro. Research in Veterinary Science, 95(2), 437-43. https://doi.org/10.1016/j.rvsc.2013.02.015
Elmowalid G, Amar AM, Ahmad AA. Nigella Sativa Seed Extract: 1. Enhancement of Sheep Macrophage Immune Functions in Vitro. Res Vet Sci. 2013;95(2):437-43. PubMed PMID: 23664216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nigella sativa seed extract: 1. Enhancement of sheep macrophage immune functions in vitro. AU - Elmowalid,Gamal, AU - Amar,Ahmad M, AU - Ahmad,Adel Attia M, Y1 - 2013/05/07/ PY - 2012/05/05/received PY - 2012/12/27/revised PY - 2013/02/22/accepted PY - 2013/5/14/entrez PY - 2013/5/15/pubmed PY - 2014/4/2/medline KW - Microbicidal activity KW - Monocytes-derived macrophages KW - N. sativa extract KW - Nitric oxide production KW - Phagocytic capacity SP - 437 EP - 43 JF - Research in veterinary science JO - Res Vet Sci VL - 95 IS - 2 N2 - Nigella sativa (N. sativa) seed, Black cumin, immunomodulatory activity has been investigated in human and mice. Little is known about the immunomodulatory effect of Nigella sativa (N. sativa) seed extract on animals' immune cells, specifically, antigen presenting cells such as macrophages. This study focused on the immunomodulatory effect of N. sativa seed extract on sheep macrophage functions in vitro. Sheep peripheral blood monocytes were isolated and derived to macrophages (MDM). The MDM were cultured with N. sativa seed extract and their morphological changes, phagocytic activity, nitric oxide production, and microbicidal activity were investigated. Marked morphological changes were observed in MDM cultured with N. sativa seed extract including cell size enlargement; increase in both cytoplasmic space and cytoplasmic granules. Significant increases in phagocytic activity to Candida albicans yeast and in number of yeast engulfed per individual MDM were observed in cells cultured with seed extract. MDM capacity to produce nitric oxide was higher in the culture media of the seed extract-cultured cells compared to the control. Interestingly, prominent enhancement in MDM microbicidal activity to yeast or bacteria was observed in MDM cultured with N. sativa seed extract confirming the potent immunostimulatory effect of the extract. From this study, it could be concluded that N. sativa seed extract can enhance macrophages' important innate immune functions that could control infectious diseases and regulate adaptive immunity. SN - 1532-2661 UR - https://www.unboundmedicine.com/medline/citation/23664216/Nigella_sativa_seed_extract:_1__Enhancement_of_sheep_macrophage_immune_functions_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0034-5288(13)00069-6 DB - PRIME DP - Unbound Medicine ER -