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Evidence of the megavirome in humans.
J Clin Virol. 2013 Jul; 57(3):191-200.JC

Abstract

BACKGROUND

Megavirales is a proposed new virus order composed of Mimivirus, Marseillevirus and closely related viruses, as well as members of the families Poxviridae, Iridoviridae, Ascoviridae, Phycodnaviridae and Asfarviridae. The Megavirales virome, which we refer to as the megavirome, has been largely neglected until now because of the use of technical procedures that have jeopardized the discovery of giant viruses, particularly the use of filters with pore sizes in the 0.2-0.45-μm range. Concurrently, there has been accumulating evidence supporting the role of Mimivirus, discovered while investigating a pneumonia outbreak using amoebal coculture, as a causative agent in pneumonia.

OBJECTIVES

In this paper, we describe the detection of sequences related to Mimivirus and Marseillevirus in the gut microbiota from a young Senegalese man. We also searched for sequences related to Megavirales in human metagenomes publicly available in sequence databases.

RESULTS

We serendipitously detected Mimivirus- and Marseillevirus-like sequences while using a new metagenomic approach targeting bacterial DNA that subsequently led to the isolation of a new member of the family Marseilleviridae, named Senegalvirus, from human stools. This discovery demonstrates the possibility of the presence of giant viruses of amoebae in humans. In addition, we detected sequences related to Megavirales members in several human metagenomes, which adds to previous findings by several groups.

CONCLUSIONS

Overall, we present convergent evidence of the presence of mimiviruses and marseilleviruses in humans. Our findings suggest that we should re-evaluate the human megavirome and investigate the prevalence, diversity and potential pathogenicity of giant viruses in humans.

Authors+Show Affiliations

Aix-Marseille Univ, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes UM 63 CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, Marseille, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23664726

Citation

Colson, Philippe, et al. "Evidence of the Megavirome in Humans." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 57, no. 3, 2013, pp. 191-200.
Colson P, Fancello L, Gimenez G, et al. Evidence of the megavirome in humans. J Clin Virol. 2013;57(3):191-200.
Colson, P., Fancello, L., Gimenez, G., Armougom, F., Desnues, C., Fournous, G., Yoosuf, N., Million, M., La Scola, B., & Raoult, D. (2013). Evidence of the megavirome in humans. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 57(3), 191-200. https://doi.org/10.1016/j.jcv.2013.03.018
Colson P, et al. Evidence of the Megavirome in Humans. J Clin Virol. 2013;57(3):191-200. PubMed PMID: 23664726.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence of the megavirome in humans. AU - Colson,Philippe, AU - Fancello,Laura, AU - Gimenez,Gregory, AU - Armougom,Fabrice, AU - Desnues,Christelle, AU - Fournous,Ghislain, AU - Yoosuf,Niyaz, AU - Million,Matthieu, AU - La Scola,Bernard, AU - Raoult,Didier, Y1 - 2013/05/08/ PY - 2013/01/07/received PY - 2013/03/14/revised PY - 2013/03/29/accepted PY - 2013/5/14/entrez PY - 2013/5/15/pubmed PY - 2013/12/16/medline SP - 191 EP - 200 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J. Clin. Virol. VL - 57 IS - 3 N2 - BACKGROUND: Megavirales is a proposed new virus order composed of Mimivirus, Marseillevirus and closely related viruses, as well as members of the families Poxviridae, Iridoviridae, Ascoviridae, Phycodnaviridae and Asfarviridae. The Megavirales virome, which we refer to as the megavirome, has been largely neglected until now because of the use of technical procedures that have jeopardized the discovery of giant viruses, particularly the use of filters with pore sizes in the 0.2-0.45-μm range. Concurrently, there has been accumulating evidence supporting the role of Mimivirus, discovered while investigating a pneumonia outbreak using amoebal coculture, as a causative agent in pneumonia. OBJECTIVES: In this paper, we describe the detection of sequences related to Mimivirus and Marseillevirus in the gut microbiota from a young Senegalese man. We also searched for sequences related to Megavirales in human metagenomes publicly available in sequence databases. RESULTS: We serendipitously detected Mimivirus- and Marseillevirus-like sequences while using a new metagenomic approach targeting bacterial DNA that subsequently led to the isolation of a new member of the family Marseilleviridae, named Senegalvirus, from human stools. This discovery demonstrates the possibility of the presence of giant viruses of amoebae in humans. In addition, we detected sequences related to Megavirales members in several human metagenomes, which adds to previous findings by several groups. CONCLUSIONS: Overall, we present convergent evidence of the presence of mimiviruses and marseilleviruses in humans. Our findings suggest that we should re-evaluate the human megavirome and investigate the prevalence, diversity and potential pathogenicity of giant viruses in humans. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/23664726/Evidence_of_the_megavirome_in_humans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(13)00124-8 DB - PRIME DP - Unbound Medicine ER -