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Secalonic acid A protects dopaminergic neurons from 1-methyl-4-phenylpyridinium (MPP⁺)-induced cell death via the mitochondrial apoptotic pathway.
Eur J Pharmacol. 2013 Aug 05; 713(1-3):58-67.EJ

Abstract

Secalonic acid A (SAA) is a natural compound found in marine fungi. We have reported that SAA can attenuate the cytotoxicity of colchicine in rat cortical neurons. Whether SAA can also inhibit the neurotoxicity of 1-methyl-4-phenylpyridinium (MPP(+)) in dopaminergic neurons has not been investigated. Here, we show that pretreatment with 1 μM SAA significantly rescued tyrosine hydroxylase (TH)-positive neurons from MPP(+)-induced neurotoxicity in primary dopaminergic neuron culture. Moreover, SAA at doses of 0.15 mg/kg and 0.75 mg/kg increased the number of dopaminergic neurons and upregulated striatal dopamine in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease mice experiments. We also show that SAA significantly attenuated cytotoxicity induced by 2.5 mM MPP(+) in SH-SY5Y cells. These results indicate that the activation of JNK, p38 mitogen activated protein kinase (MAPK) and caspase-3 during apoptosis triggered by MPP(+) could be suppressed by SAA; on the other hand, an MPP(+)-induced increase in the expression of Bax in SH-SY5Y cells was blocked by SAA. These results indicate that inhibition of the phosphorylation of JNK and p38 MAPK, down-regulation of Bax expression, and suppression of caspase-3 activation are involved in the protective effects of SAA against MPP(+) toxicity in SH-SY5Y cells. SAA may rescue dopaminergic neurons from MPP(+)-induced cell death through the mitochondrial apoptotic pathway.

Authors+Show Affiliations

Institute of Health and Environmental Medicine, Academy of Military Medical Science, 27 Taiping Road, Beijing 100850, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23665112

Citation

Zhai, Aifeng, et al. "Secalonic Acid a Protects Dopaminergic Neurons From 1-methyl-4-phenylpyridinium (MPP⁺)-induced Cell Death Via the Mitochondrial Apoptotic Pathway." European Journal of Pharmacology, vol. 713, no. 1-3, 2013, pp. 58-67.
Zhai A, Zhu X, Wang X, et al. Secalonic acid A protects dopaminergic neurons from 1-methyl-4-phenylpyridinium (MPP⁺)-induced cell death via the mitochondrial apoptotic pathway. Eur J Pharmacol. 2013;713(1-3):58-67.
Zhai, A., Zhu, X., Wang, X., Chen, R., & Wang, H. (2013). Secalonic acid A protects dopaminergic neurons from 1-methyl-4-phenylpyridinium (MPP⁺)-induced cell death via the mitochondrial apoptotic pathway. European Journal of Pharmacology, 713(1-3), 58-67. https://doi.org/10.1016/j.ejphar.2013.04.029
Zhai A, et al. Secalonic Acid a Protects Dopaminergic Neurons From 1-methyl-4-phenylpyridinium (MPP⁺)-induced Cell Death Via the Mitochondrial Apoptotic Pathway. Eur J Pharmacol. 2013 Aug 5;713(1-3):58-67. PubMed PMID: 23665112.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Secalonic acid A protects dopaminergic neurons from 1-methyl-4-phenylpyridinium (MPP⁺)-induced cell death via the mitochondrial apoptotic pathway. AU - Zhai,Aifeng, AU - Zhu,Xiaonan, AU - Wang,Xuelan, AU - Chen,Ruzhu, AU - Wang,Hai, Y1 - 2013/05/09/ PY - 2012/12/09/received PY - 2013/04/11/revised PY - 2013/04/18/accepted PY - 2013/5/14/entrez PY - 2013/5/15/pubmed PY - 2014/1/15/medline SP - 58 EP - 67 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 713 IS - 1-3 N2 - Secalonic acid A (SAA) is a natural compound found in marine fungi. We have reported that SAA can attenuate the cytotoxicity of colchicine in rat cortical neurons. Whether SAA can also inhibit the neurotoxicity of 1-methyl-4-phenylpyridinium (MPP(+)) in dopaminergic neurons has not been investigated. Here, we show that pretreatment with 1 μM SAA significantly rescued tyrosine hydroxylase (TH)-positive neurons from MPP(+)-induced neurotoxicity in primary dopaminergic neuron culture. Moreover, SAA at doses of 0.15 mg/kg and 0.75 mg/kg increased the number of dopaminergic neurons and upregulated striatal dopamine in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease mice experiments. We also show that SAA significantly attenuated cytotoxicity induced by 2.5 mM MPP(+) in SH-SY5Y cells. These results indicate that the activation of JNK, p38 mitogen activated protein kinase (MAPK) and caspase-3 during apoptosis triggered by MPP(+) could be suppressed by SAA; on the other hand, an MPP(+)-induced increase in the expression of Bax in SH-SY5Y cells was blocked by SAA. These results indicate that inhibition of the phosphorylation of JNK and p38 MAPK, down-regulation of Bax expression, and suppression of caspase-3 activation are involved in the protective effects of SAA against MPP(+) toxicity in SH-SY5Y cells. SAA may rescue dopaminergic neurons from MPP(+)-induced cell death through the mitochondrial apoptotic pathway. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/23665112/Secalonic_acid_A_protects_dopaminergic_neurons_from_1_methyl_4_phenylpyridinium__MPP���__induced_cell_death_via_the_mitochondrial_apoptotic_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(13)00334-8 DB - PRIME DP - Unbound Medicine ER -