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Antimicrobial and acetylcholinesterase inhibitory activities of Buddleja salviifolia (L.) Lam. leaf extracts and isolated compounds.
J Ethnopharmacol. 2013 Jul 09; 148(2):515-20.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Buddleja salviifolia leaves are used for the treatment of eye infections and neurodegenerative conditions by various tribes in South Africa.

AIM OF STUDY

This study was designed to isolate the phenolic constituents from the leaf extracts of Buddleja salviifolia and evaluate their antimicrobial and acetylcholinesterase (AChE) activities.

MATERIAL AND METHODS

Three phenolic compounds were isolated from the ethyl acetate fraction of a 20% aqueous methanol leaf extract of Buddleja salviifolia using Sephadex LH-20 and silica gel columns. Structure elucidation of the isolated compounds was carried out using spectroscopic techniques: mass spectrometry (ESI-TOF-MS) and NMR (1D and 2D). The extracts and isolated compounds were evaluated for antimicrobial and acetylcholinesterase activities using the microdilution technique. The bacteria used for the antimicrobial assays were Gram-positive Bacillus subtilis and Staphylococcus aureus and Gram-negative Escherichia coli and Klebsiella pneumoniae.

RESULTS

The isolated compounds were characterized as: 4'-hydroxyphenyl ethyl vanillate (1) a new natural product, acteoside (2) and quercetin (3). The crude extract, fractions and the isolated compounds from the leaves of the plant exhibited a broad spectrum of antibacterial activity. The EtOAc fraction exhibited good activity against Bacillus subtilis and Staphylococcus aureus with MIC values ranging from 780.0 to 390.0 µg/mL. Isolated compound 2 exhibited good activity against Staphylococcus aureus with an MIC value of 62.5 µg/mL. The hexane and DCM fractions of leaves showed the best activity against Candida albicans with MIC and MFC values of 390.0 µg/mL. In the AChE inhibitory test, among the tested extracts, the hexane fraction was the most potent with an IC50 value of 107.4 µg/mL, whereas for the isolated compounds, it was compound (3) (quercetin) with an IC50 value of 66.8 µg/mL.

CONCLUSIONS

Activities demonstrated by the extracts and isolated compounds support the ethnopharmacological use of Buddleja salviifolia against eye infections and neurodegenerative diseases.

Authors+Show Affiliations

Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23665162

Citation

Pendota, S C., et al. "Antimicrobial and Acetylcholinesterase Inhibitory Activities of Buddleja Salviifolia (L.) Lam. Leaf Extracts and Isolated Compounds." Journal of Ethnopharmacology, vol. 148, no. 2, 2013, pp. 515-20.
Pendota SC, Aderogba MA, Ndhlala AR, et al. Antimicrobial and acetylcholinesterase inhibitory activities of Buddleja salviifolia (L.) Lam. leaf extracts and isolated compounds. J Ethnopharmacol. 2013;148(2):515-20.
Pendota, S. C., Aderogba, M. A., Ndhlala, A. R., & Van Staden, J. (2013). Antimicrobial and acetylcholinesterase inhibitory activities of Buddleja salviifolia (L.) Lam. leaf extracts and isolated compounds. Journal of Ethnopharmacology, 148(2), 515-20. https://doi.org/10.1016/j.jep.2013.04.047
Pendota SC, et al. Antimicrobial and Acetylcholinesterase Inhibitory Activities of Buddleja Salviifolia (L.) Lam. Leaf Extracts and Isolated Compounds. J Ethnopharmacol. 2013 Jul 9;148(2):515-20. PubMed PMID: 23665162.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial and acetylcholinesterase inhibitory activities of Buddleja salviifolia (L.) Lam. leaf extracts and isolated compounds. AU - Pendota,S C, AU - Aderogba,M A, AU - Ndhlala,A R, AU - Van Staden,J, Y1 - 2013/05/07/ PY - 2013/02/13/received PY - 2013/04/17/revised PY - 2013/04/21/accepted PY - 2013/5/14/entrez PY - 2013/5/15/pubmed PY - 2014/3/7/medline SP - 515 EP - 20 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 148 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Buddleja salviifolia leaves are used for the treatment of eye infections and neurodegenerative conditions by various tribes in South Africa. AIM OF STUDY: This study was designed to isolate the phenolic constituents from the leaf extracts of Buddleja salviifolia and evaluate their antimicrobial and acetylcholinesterase (AChE) activities. MATERIAL AND METHODS: Three phenolic compounds were isolated from the ethyl acetate fraction of a 20% aqueous methanol leaf extract of Buddleja salviifolia using Sephadex LH-20 and silica gel columns. Structure elucidation of the isolated compounds was carried out using spectroscopic techniques: mass spectrometry (ESI-TOF-MS) and NMR (1D and 2D). The extracts and isolated compounds were evaluated for antimicrobial and acetylcholinesterase activities using the microdilution technique. The bacteria used for the antimicrobial assays were Gram-positive Bacillus subtilis and Staphylococcus aureus and Gram-negative Escherichia coli and Klebsiella pneumoniae. RESULTS: The isolated compounds were characterized as: 4'-hydroxyphenyl ethyl vanillate (1) a new natural product, acteoside (2) and quercetin (3). The crude extract, fractions and the isolated compounds from the leaves of the plant exhibited a broad spectrum of antibacterial activity. The EtOAc fraction exhibited good activity against Bacillus subtilis and Staphylococcus aureus with MIC values ranging from 780.0 to 390.0 µg/mL. Isolated compound 2 exhibited good activity against Staphylococcus aureus with an MIC value of 62.5 µg/mL. The hexane and DCM fractions of leaves showed the best activity against Candida albicans with MIC and MFC values of 390.0 µg/mL. In the AChE inhibitory test, among the tested extracts, the hexane fraction was the most potent with an IC50 value of 107.4 µg/mL, whereas for the isolated compounds, it was compound (3) (quercetin) with an IC50 value of 66.8 µg/mL. CONCLUSIONS: Activities demonstrated by the extracts and isolated compounds support the ethnopharmacological use of Buddleja salviifolia against eye infections and neurodegenerative diseases. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/23665162/Antimicrobial_and_acetylcholinesterase_inhibitory_activities_of_Buddleja_salviifolia__L___Lam__leaf_extracts_and_isolated_compounds_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00326-7 DB - PRIME DP - Unbound Medicine ER -