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Study the catalyzing mechanism of dissolved redox mediators on bio-denitrification by metabolic inhibitors.
Bioresour Technol. 2013 Jul; 140:22-7.BT

Abstract

The effect of dissolved redox mediators on denitrification by Paracoccus versutus sp. GW1 were studied with α-AQS, AQS, 1,5-AQDS, AQDS and 2,7-AQDS. AQDS presented the best catalytic effect which increased denitrification rate by 1.5-fold with 0.17 mmol L(-1). Denitrification rate was found to be pseudo-zero order with 0-0.32 mmol L(-1) AQDS (k=19.874C AQDS+10.301 (R(2)=0.9984)). The accelerating mechanism for respiratory chain was also explored by using metabolic inhibitors. Addition of AQDS eased the inhibitions of rotenone, dicumarol, sodium azide on respiratory chain, and enhanced the nitrite accumulation during bio-denitrification process. AQDS exhibited slight catalytic effect on nitrite denitrification. These results allowed us to deduce the accelerating site of AQDS on the denitrification respiratory chain. Increased nitrate denitrification by AQDS might mainly attribute to the accelerated electrons transfer from NADH to nitrate reductase via complex I and ubiquinone/ubiqunol. This study provided further understanding of the mechanism of redox mediators on denitrification.

Authors+Show Affiliations

School of Environmental Science and Engineering, Hebei University of Science and Technology, Yuhua East Road 70#, Shijiazhuang City 050018, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23669099

Citation

Xi, Zhenhua, et al. "Study the Catalyzing Mechanism of Dissolved Redox Mediators On Bio-denitrification By Metabolic Inhibitors." Bioresource Technology, vol. 140, 2013, pp. 22-7.
Xi Z, Guo J, Lian J, et al. Study the catalyzing mechanism of dissolved redox mediators on bio-denitrification by metabolic inhibitors. Bioresour Technol. 2013;140:22-7.
Xi, Z., Guo, J., Lian, J., Li, H., Zhao, L., Liu, X., Zhang, C., & Yang, J. (2013). Study the catalyzing mechanism of dissolved redox mediators on bio-denitrification by metabolic inhibitors. Bioresource Technology, 140, 22-7. https://doi.org/10.1016/j.biortech.2013.04.065
Xi Z, et al. Study the Catalyzing Mechanism of Dissolved Redox Mediators On Bio-denitrification By Metabolic Inhibitors. Bioresour Technol. 2013;140:22-7. PubMed PMID: 23669099.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study the catalyzing mechanism of dissolved redox mediators on bio-denitrification by metabolic inhibitors. AU - Xi,Zhenhua, AU - Guo,Jianbo, AU - Lian,Jing, AU - Li,Haibo, AU - Zhao,Lijun, AU - Liu,Xiaoyu, AU - Zhang,Chenxiao, AU - Yang,Jingliang, Y1 - 2013/04/24/ PY - 2013/01/12/received PY - 2013/04/15/revised PY - 2013/04/16/accepted PY - 2013/5/15/entrez PY - 2013/5/15/pubmed PY - 2014/1/15/medline SP - 22 EP - 7 JF - Bioresource technology JO - Bioresour. Technol. VL - 140 N2 - The effect of dissolved redox mediators on denitrification by Paracoccus versutus sp. GW1 were studied with α-AQS, AQS, 1,5-AQDS, AQDS and 2,7-AQDS. AQDS presented the best catalytic effect which increased denitrification rate by 1.5-fold with 0.17 mmol L(-1). Denitrification rate was found to be pseudo-zero order with 0-0.32 mmol L(-1) AQDS (k=19.874C AQDS+10.301 (R(2)=0.9984)). The accelerating mechanism for respiratory chain was also explored by using metabolic inhibitors. Addition of AQDS eased the inhibitions of rotenone, dicumarol, sodium azide on respiratory chain, and enhanced the nitrite accumulation during bio-denitrification process. AQDS exhibited slight catalytic effect on nitrite denitrification. These results allowed us to deduce the accelerating site of AQDS on the denitrification respiratory chain. Increased nitrate denitrification by AQDS might mainly attribute to the accelerated electrons transfer from NADH to nitrate reductase via complex I and ubiquinone/ubiqunol. This study provided further understanding of the mechanism of redox mediators on denitrification. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/23669099/Study_the_catalyzing_mechanism_of_dissolved_redox_mediators_on_bio_denitrification_by_metabolic_inhibitors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(13)00679-2 DB - PRIME DP - Unbound Medicine ER -