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Fetal skeletal muscle progenitors have regenerative capacity after intramuscular engraftment in dystrophin deficient mice.
PLoS One. 2013; 8(5):e63016.Plos

Abstract

Muscle satellite cells (SCs) are stem cells that reside in skeletal muscles and contribute to regeneration upon muscle injury. SCs arise from skeletal muscle progenitors expressing transcription factors Pax3 and/or Pax7 during embryogenesis in mice. However, it is unclear whether these fetal progenitors possess regenerative ability when transplanted in adult muscle. Here we address this question by investigating whether fetal skeletal muscle progenitors (FMPs) isolated from Pax3(GFP/+) embryos have the capacity to regenerate muscle after engraftment into Dystrophin-deficient mice, a model of Duchenne muscular dystrophy. The capacity of FMPs to engraft and enter the myogenic program in regenerating muscle was compared with that of SCs derived from adult Pax3(GFP/+) mice. Transplanted FMPs contributed to the reconstitution of damaged myofibers in Dystrophin-deficient mice. However, despite FMPs and SCs having similar myogenic ability in culture, the regenerative ability of FMPs was less than that of SCs in vivo. FMPs that had activated MyoD engrafted more efficiently to regenerate myofibers than MyoD-negative FMPs. Transcriptome and surface marker analyses of these cells suggest the importance of myogenic priming for the efficient myogenic engraftment. Our findings suggest the regenerative capability of FMPs in the context of muscle repair and cell therapy for degenerative muscle disease.

Authors+Show Affiliations

Department of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23671652

Citation

Sakai, Hiroshi, et al. "Fetal Skeletal Muscle Progenitors Have Regenerative Capacity After Intramuscular Engraftment in Dystrophin Deficient Mice." PloS One, vol. 8, no. 5, 2013, pp. e63016.
Sakai H, Sato T, Sakurai H, et al. Fetal skeletal muscle progenitors have regenerative capacity after intramuscular engraftment in dystrophin deficient mice. PLoS ONE. 2013;8(5):e63016.
Sakai, H., Sato, T., Sakurai, H., Yamamoto, T., Hanaoka, K., Montarras, D., & Sehara-Fujisawa, A. (2013). Fetal skeletal muscle progenitors have regenerative capacity after intramuscular engraftment in dystrophin deficient mice. PloS One, 8(5), e63016. https://doi.org/10.1371/journal.pone.0063016
Sakai H, et al. Fetal Skeletal Muscle Progenitors Have Regenerative Capacity After Intramuscular Engraftment in Dystrophin Deficient Mice. PLoS ONE. 2013;8(5):e63016. PubMed PMID: 23671652.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fetal skeletal muscle progenitors have regenerative capacity after intramuscular engraftment in dystrophin deficient mice. AU - Sakai,Hiroshi, AU - Sato,Takahiko, AU - Sakurai,Hidetoshi, AU - Yamamoto,Takuya, AU - Hanaoka,Kazunori, AU - Montarras,Didier, AU - Sehara-Fujisawa,Atsuko, Y1 - 2013/05/09/ PY - 2013/01/08/received PY - 2013/03/27/accepted PY - 2013/5/15/entrez PY - 2013/5/15/pubmed PY - 2013/12/25/medline SP - e63016 EP - e63016 JF - PloS one JO - PLoS ONE VL - 8 IS - 5 N2 - Muscle satellite cells (SCs) are stem cells that reside in skeletal muscles and contribute to regeneration upon muscle injury. SCs arise from skeletal muscle progenitors expressing transcription factors Pax3 and/or Pax7 during embryogenesis in mice. However, it is unclear whether these fetal progenitors possess regenerative ability when transplanted in adult muscle. Here we address this question by investigating whether fetal skeletal muscle progenitors (FMPs) isolated from Pax3(GFP/+) embryos have the capacity to regenerate muscle after engraftment into Dystrophin-deficient mice, a model of Duchenne muscular dystrophy. The capacity of FMPs to engraft and enter the myogenic program in regenerating muscle was compared with that of SCs derived from adult Pax3(GFP/+) mice. Transplanted FMPs contributed to the reconstitution of damaged myofibers in Dystrophin-deficient mice. However, despite FMPs and SCs having similar myogenic ability in culture, the regenerative ability of FMPs was less than that of SCs in vivo. FMPs that had activated MyoD engrafted more efficiently to regenerate myofibers than MyoD-negative FMPs. Transcriptome and surface marker analyses of these cells suggest the importance of myogenic priming for the efficient myogenic engraftment. Our findings suggest the regenerative capability of FMPs in the context of muscle repair and cell therapy for degenerative muscle disease. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23671652/Fetal_skeletal_muscle_progenitors_have_regenerative_capacity_after_intramuscular_engraftment_in_dystrophin_deficient_mice_ L2 - http://dx.plos.org/10.1371/journal.pone.0063016 DB - PRIME DP - Unbound Medicine ER -