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Simultaneous quantification of amphetamine, opiates, ketamine and relative metabolites in urine for confirmatory analysis by liquid chromatography tandem mass spectrometry.

Abstract

The rise in amphetamine, ketamine and opiates abuse in Taiwan has created a need for a reliable confirmatory assay. A method that combines superficially porous liquid chromatography tandem mass spectrometry (LC-MS/MS) with solid-phase extraction (SPE) was developed for the simultaneous quantification of amphetamine, 3,4-methylenedioxymethamphetamine (MDMA), ketamine, opiates, and their corresponding metabolites in urine. The total run time of the method was 6.7min including equilibration time. The method was validated in accordance with the European Commission (EC) Decision 2002/642/EC. The within- and between-day precision was below 13.6% and the accuracy ranged from -17.1% to +9.9% for all analytes. Ion suppression was observed but compensated by using deuterated internal standards. No carryover was detected and the analytes were stable at room temperature for 16h, and for 72h at 4°C, and three-thaw cycles. The method was further validated by comparison with a reference gas chromatography-mass spectrometry (GC-MS) method, using 52 authentic urine samples. The results indicated that for the target analytes studied, the LC-MS/MS analysis was as precise, accurate, and specific as the GC-MS method. In conclusion, the present LC-MS/MS method is robust and reliable, and suitable for use as a confirmation assay in the simultaneous urine drug testing and quantification of amphetamines, ketamines, and opiates.

Authors+Show Affiliations

Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, Taiwan. rita0107@mail.tcu.edu.twNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23672816

Citation

Lin, Huei-Ru, et al. "Simultaneous Quantification of Amphetamine, Opiates, Ketamine and Relative Metabolites in Urine for Confirmatory Analysis By Liquid Chromatography Tandem Mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 929, 2013, pp. 133-41.
Lin HR, Choi KI, Lin TC, et al. Simultaneous quantification of amphetamine, opiates, ketamine and relative metabolites in urine for confirmatory analysis by liquid chromatography tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2013;929:133-41.
Lin, H. R., Choi, K. I., Lin, T. C., & Hu, A. (2013). Simultaneous quantification of amphetamine, opiates, ketamine and relative metabolites in urine for confirmatory analysis by liquid chromatography tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 929, 133-41. https://doi.org/10.1016/j.jchromb.2013.03.022
Lin HR, et al. Simultaneous Quantification of Amphetamine, Opiates, Ketamine and Relative Metabolites in Urine for Confirmatory Analysis By Liquid Chromatography Tandem Mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jun 15;929:133-41. PubMed PMID: 23672816.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantification of amphetamine, opiates, ketamine and relative metabolites in urine for confirmatory analysis by liquid chromatography tandem mass spectrometry. AU - Lin,Huei-Ru, AU - Choi,Ka-Ian, AU - Lin,Tzu-Chieh, AU - Hu,Anren, Y1 - 2013/04/18/ PY - 2012/11/13/received PY - 2013/03/12/revised PY - 2013/03/24/accepted PY - 2013/5/16/entrez PY - 2013/5/16/pubmed PY - 2013/10/25/medline SP - 133 EP - 41 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 929 N2 - The rise in amphetamine, ketamine and opiates abuse in Taiwan has created a need for a reliable confirmatory assay. A method that combines superficially porous liquid chromatography tandem mass spectrometry (LC-MS/MS) with solid-phase extraction (SPE) was developed for the simultaneous quantification of amphetamine, 3,4-methylenedioxymethamphetamine (MDMA), ketamine, opiates, and their corresponding metabolites in urine. The total run time of the method was 6.7min including equilibration time. The method was validated in accordance with the European Commission (EC) Decision 2002/642/EC. The within- and between-day precision was below 13.6% and the accuracy ranged from -17.1% to +9.9% for all analytes. Ion suppression was observed but compensated by using deuterated internal standards. No carryover was detected and the analytes were stable at room temperature for 16h, and for 72h at 4°C, and three-thaw cycles. The method was further validated by comparison with a reference gas chromatography-mass spectrometry (GC-MS) method, using 52 authentic urine samples. The results indicated that for the target analytes studied, the LC-MS/MS analysis was as precise, accurate, and specific as the GC-MS method. In conclusion, the present LC-MS/MS method is robust and reliable, and suitable for use as a confirmation assay in the simultaneous urine drug testing and quantification of amphetamines, ketamines, and opiates. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/23672816/Simultaneous_quantification_of_amphetamine_opiates_ketamine_and_relative_metabolites_in_urine_for_confirmatory_analysis_by_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(13)00180-3 DB - PRIME DP - Unbound Medicine ER -