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Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife.
J Antimicrob Chemother. 2013 Oct; 68(10):2222-5.JA

Abstract

OBJECTIVES

A recently identified mecA homologue, mecC, in methicillin-resistant Staphylococcus aureus (MRSA) has been isolated from humans and different animal hosts. The aim of this study was to determine antimicrobial resistance and provide molecular characterization of MRSA and methicillin-resistant non-Staphylococcus aureus staphylococci (MRnSA) isolated from wildlife that carried the gene mecC.

METHODS

Five S. aureus and one coagulase-negative Staphylococcus isolate displaying phenotypic oxacillin resistance, but not recognized with conventional PCR for mecA, were further characterized by a polyphasic approach. The presence of mecC in all isolates was determined using specific PCR. PCR targeting Panton-Valentine leucocidin (PVL) genes of MRSA was performed. MRSA isolates were genotyped by spa typing and multilocus sequence typing. All isolates were genotyped by staphylococcal cassette chromosome mec (SCCmec) typing. 16S rDNA sequence analysis for MRnSA identification was performed. Antimicrobial susceptibility testing was performed for all isolates.

RESULTS

All five MRSA isolates contained the mecC gene, were PVL negative, carried SCCmec type XI and belonged to ST130 (where ST stands for sequence type), with spa types t843, t10513 or t3256, or to ST2620, with spa type t4335. The MRnSA isolate, most closely related to Staphylococcus stepanovicii, carried mecA and blaZ genes related to SCCmec XI. MRSA isolates exhibited resistance to the β-lactams only.

CONCLUSIONS

The MRSA isolates described in this study represent the first detection of mecC-positive MRSA in a European otter (Lutra lutra) and a European brown hare (Lepus europaeus). The MRnSA isolate represents the first isolation of MRnSA from a Eurasian lynx (Lynx lynx).

Authors+Show Affiliations

Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23674764

Citation

Loncaric, Igor, et al. "Characterization of Methicillin-resistant Staphylococcus Spp. Carrying the mecC Gene, Isolated From Wildlife." The Journal of Antimicrobial Chemotherapy, vol. 68, no. 10, 2013, pp. 2222-5.
Loncaric I, Kübber-Heiss A, Posautz A, et al. Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife. J Antimicrob Chemother. 2013;68(10):2222-5.
Loncaric, I., Kübber-Heiss, A., Posautz, A., Stalder, G. L., Hoffmann, D., Rosengarten, R., & Walzer, C. (2013). Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife. The Journal of Antimicrobial Chemotherapy, 68(10), 2222-5. https://doi.org/10.1093/jac/dkt186
Loncaric I, et al. Characterization of Methicillin-resistant Staphylococcus Spp. Carrying the mecC Gene, Isolated From Wildlife. J Antimicrob Chemother. 2013;68(10):2222-5. PubMed PMID: 23674764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife. AU - Loncaric,Igor, AU - Kübber-Heiss,Anna, AU - Posautz,Annika, AU - Stalder,Gabrielle L, AU - Hoffmann,Daniel, AU - Rosengarten,Renate, AU - Walzer,Chris, Y1 - 2013/05/14/ PY - 2013/5/16/entrez PY - 2013/5/16/pubmed PY - 2014/3/13/medline KW - MLST KW - MRSA KW - antimicrobial resistance KW - spa typing SP - 2222 EP - 5 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 68 IS - 10 N2 - OBJECTIVES: A recently identified mecA homologue, mecC, in methicillin-resistant Staphylococcus aureus (MRSA) has been isolated from humans and different animal hosts. The aim of this study was to determine antimicrobial resistance and provide molecular characterization of MRSA and methicillin-resistant non-Staphylococcus aureus staphylococci (MRnSA) isolated from wildlife that carried the gene mecC. METHODS: Five S. aureus and one coagulase-negative Staphylococcus isolate displaying phenotypic oxacillin resistance, but not recognized with conventional PCR for mecA, were further characterized by a polyphasic approach. The presence of mecC in all isolates was determined using specific PCR. PCR targeting Panton-Valentine leucocidin (PVL) genes of MRSA was performed. MRSA isolates were genotyped by spa typing and multilocus sequence typing. All isolates were genotyped by staphylococcal cassette chromosome mec (SCCmec) typing. 16S rDNA sequence analysis for MRnSA identification was performed. Antimicrobial susceptibility testing was performed for all isolates. RESULTS: All five MRSA isolates contained the mecC gene, were PVL negative, carried SCCmec type XI and belonged to ST130 (where ST stands for sequence type), with spa types t843, t10513 or t3256, or to ST2620, with spa type t4335. The MRnSA isolate, most closely related to Staphylococcus stepanovicii, carried mecA and blaZ genes related to SCCmec XI. MRSA isolates exhibited resistance to the β-lactams only. CONCLUSIONS: The MRSA isolates described in this study represent the first detection of mecC-positive MRSA in a European otter (Lutra lutra) and a European brown hare (Lepus europaeus). The MRnSA isolate represents the first isolation of MRnSA from a Eurasian lynx (Lynx lynx). SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/23674764/Characterization_of_methicillin_resistant_Staphylococcus_spp__carrying_the_mecC_gene_isolated_from_wildlife_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkt186 DB - PRIME DP - Unbound Medicine ER -