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Spectrophotometric and Spectrofluorimetric Methods for the Determination of Dothiepin Hydrochloride in its Pure and Dosage Forms using Eosin.
Int J Biomed Sci. 2010 Dec; 6(4):327-34.IJ

Abstract

Spectrophotometric and spectrofluorimetric methods were developed for the determination of dothiepin hydrochloride (DOP) in different dosage forms. The spectrophotometric method (Method I) is based on formation of a binary complex with eosin at 540 nm in acetate buffer of pH3.7. The absorbance-concentration plot is rectilinear over the range 1-10 μg/mL with LOD of 0.18 μg/mL and LOQ of 0.54 μg/mL. The spectroflurimetric method (Method II) is based on the quantitative quenching effect of Dothiepin on the native fluorescence of eosin at the same pH. The quenching of the fluorescence of eosin was measured at 543 nm after excitation at 304 nm. The fluorescence-concentration plot is rectilinear over the range 0.3-8 μg/ mL with LOD of 0.11 μg/mL and LOQ of 0.34 μg/mL. The proposed methods were successfully applied to the analysis of commercial tablets and capsules containing the drug. Statistical comparison of the results with those of the reference method revealed good agreement and proved that there were no significant differences in the accuracy and precision between the two methods respectively.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23675210

Citation

Walash, M I., et al. "Spectrophotometric and Spectrofluorimetric Methods for the Determination of Dothiepin Hydrochloride in Its Pure and Dosage Forms Using Eosin." International Journal of Biomedical Science : IJBS, vol. 6, no. 4, 2010, pp. 327-34.
Walash MI, Belal F, El-Enany N, et al. Spectrophotometric and Spectrofluorimetric Methods for the Determination of Dothiepin Hydrochloride in its Pure and Dosage Forms using Eosin. Int J Biomed Sci. 2010;6(4):327-34.
Walash, M. I., Belal, F., El-Enany, N., & Elmansi, H. (2010). Spectrophotometric and Spectrofluorimetric Methods for the Determination of Dothiepin Hydrochloride in its Pure and Dosage Forms using Eosin. International Journal of Biomedical Science : IJBS, 6(4), 327-34.
Walash MI, et al. Spectrophotometric and Spectrofluorimetric Methods for the Determination of Dothiepin Hydrochloride in Its Pure and Dosage Forms Using Eosin. Int J Biomed Sci. 2010;6(4):327-34. PubMed PMID: 23675210.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spectrophotometric and Spectrofluorimetric Methods for the Determination of Dothiepin Hydrochloride in its Pure and Dosage Forms using Eosin. AU - Walash,M I, AU - Belal,F, AU - El-Enany,N, AU - Elmansi,H, PY - 2010/05/27/received PY - 2010/06/28/accepted PY - 2013/5/16/entrez PY - 2010/12/1/pubmed PY - 2010/12/1/medline KW - Dothiepin hydrochloride (DOP) KW - dosage forms KW - eosin KW - spectroflurimetry KW - spectrophotometry SP - 327 EP - 34 JF - International journal of biomedical science : IJBS JO - Int J Biomed Sci VL - 6 IS - 4 N2 - Spectrophotometric and spectrofluorimetric methods were developed for the determination of dothiepin hydrochloride (DOP) in different dosage forms. The spectrophotometric method (Method I) is based on formation of a binary complex with eosin at 540 nm in acetate buffer of pH3.7. The absorbance-concentration plot is rectilinear over the range 1-10 μg/mL with LOD of 0.18 μg/mL and LOQ of 0.54 μg/mL. The spectroflurimetric method (Method II) is based on the quantitative quenching effect of Dothiepin on the native fluorescence of eosin at the same pH. The quenching of the fluorescence of eosin was measured at 543 nm after excitation at 304 nm. The fluorescence-concentration plot is rectilinear over the range 0.3-8 μg/ mL with LOD of 0.11 μg/mL and LOQ of 0.34 μg/mL. The proposed methods were successfully applied to the analysis of commercial tablets and capsules containing the drug. Statistical comparison of the results with those of the reference method revealed good agreement and proved that there were no significant differences in the accuracy and precision between the two methods respectively. SN - 1550-9702 UR - https://www.unboundmedicine.com/medline/citation/23675210/Spectrophotometric_and_Spectrofluorimetric_Methods_for_the_Determination_of_Dothiepin_Hydrochloride_in_its_Pure_and_Dosage_Forms_using_Eosin_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23675210/ DB - PRIME DP - Unbound Medicine ER -
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