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Effects induced in neutrophils by a precursor of triplet acetone.
Photochem Photobiol 1990; 51(6):713-7PP

Abstract

The addition of a precursor of the enol form of isobutanal to neutrophils results in formation of triplet acetone, as attested to by emission from appropriate acceptors and cell damage (Nascimento et al., 1986 Biochim. Biophys. Acta 888, 337-342). The present study confirms the formation of triplet acetone by detection of the direct emission (lambda max 430 nm) and differentiates between effects produced by triplet acetone and by the enol substrate itself. Thus, triplet acetone: (1) enhances the release of ribonucleic acid; (2) promotes lipid peroxidation (N3(-)-inhibitable formation of thiobarbituric acid reactive products and concomitant light emission peaking at 480-500 nm); (3) increases myeloperoxidase activity, presumable as a result of damage and consequent increased exposure of the enzyme. On the other hand, the enol greatly enhances the release of protein(s) into the medium. These results confirm the utility of the neutrophil as a model system for the study of chemiexcitation processes induced at the cellular level. They also provide the first demonstration that an excited species formed at the cellular level may induce release of nucleic acids, thus reflecting the occurrence of deleterious processes in situ.

Authors+Show Affiliations

Departamento de Bioquímica, Universidade de São Paulo, Brazil.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2367565

Citation

Escobar, J A., et al. "Effects Induced in Neutrophils By a Precursor of Triplet Acetone." Photochemistry and Photobiology, vol. 51, no. 6, 1990, pp. 713-7.
Escobar JA, Cilento G, Nascimento AL. Effects induced in neutrophils by a precursor of triplet acetone. Photochem Photobiol. 1990;51(6):713-7.
Escobar, J. A., Cilento, G., & Nascimento, A. L. (1990). Effects induced in neutrophils by a precursor of triplet acetone. Photochemistry and Photobiology, 51(6), pp. 713-7.
Escobar JA, Cilento G, Nascimento AL. Effects Induced in Neutrophils By a Precursor of Triplet Acetone. Photochem Photobiol. 1990;51(6):713-7. PubMed PMID: 2367565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects induced in neutrophils by a precursor of triplet acetone. AU - Escobar,J A, AU - Cilento,G, AU - Nascimento,A L, PY - 1990/6/1/pubmed PY - 1990/6/1/medline PY - 1990/6/1/entrez SP - 713 EP - 7 JF - Photochemistry and photobiology JO - Photochem. Photobiol. VL - 51 IS - 6 N2 - The addition of a precursor of the enol form of isobutanal to neutrophils results in formation of triplet acetone, as attested to by emission from appropriate acceptors and cell damage (Nascimento et al., 1986 Biochim. Biophys. Acta 888, 337-342). The present study confirms the formation of triplet acetone by detection of the direct emission (lambda max 430 nm) and differentiates between effects produced by triplet acetone and by the enol substrate itself. Thus, triplet acetone: (1) enhances the release of ribonucleic acid; (2) promotes lipid peroxidation (N3(-)-inhibitable formation of thiobarbituric acid reactive products and concomitant light emission peaking at 480-500 nm); (3) increases myeloperoxidase activity, presumable as a result of damage and consequent increased exposure of the enzyme. On the other hand, the enol greatly enhances the release of protein(s) into the medium. These results confirm the utility of the neutrophil as a model system for the study of chemiexcitation processes induced at the cellular level. They also provide the first demonstration that an excited species formed at the cellular level may induce release of nucleic acids, thus reflecting the occurrence of deleterious processes in situ. SN - 0031-8655 UR - https://www.unboundmedicine.com/medline/citation/2367565/Effects_induced_in_neutrophils_by_a_precursor_of_triplet_acetone_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0031-8655&date=1990&volume=51&issue=6&spage=713 DB - PRIME DP - Unbound Medicine ER -