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Improvement of the diagnostic sensitivity of scrub typhus using a mixture of recombinant antigens derived from Orientia tsutsugamushi serotypes.
J Korean Med Sci. 2013 May; 28(5):672-9.JK

Abstract

Diagnosis of scrub typhus is difficult because its symptoms are very similar to other acute febrile illnesses, such as leptospirosis, murine typhus, and other viral hemorrhagic fevers. To differentiate scrub typhus from other acute febrile diseases, a rapid and reliable serological diagnosis is important. We have developed a chimeric recombinant antigen cr56 and two other recombinant antigens, r21 and kr56, from various serotypes of Orientia tsutsugamushi. They were tested for the detection of antibodies against O. tsutsugamushi in the patient's serum samples using enzyme-linked immunosorbent assay (ELISA) and dot-blot analyses. As of conventional immunofluorescence assay (IFA), when the mixture of these three recombinant antigens was used, both sensitivity and specificity of the recombinant antigens were increased up to 98% in IgM and IgG at ELISA and dot blotting. Additionally, both sensitivity and specificity by detection of IgM and IgG antibodies at rapid diagnostic test (RDT), using the mixture of three antigens and gold conjugated antibodies, were 99%. Our results suggest the use of mixture of these recombinant antigen proteins in ELISA or RDT is suitable as a diagnostic test for scrub typhus.

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Authors+Show Affiliations

Kim YJ
Immunemed, Chuncheon, Korea.
Yeo SJ
No affiliation info available
Park SJ
No affiliation info available
Woo YJ
No affiliation info available
Kim MW
No affiliation info available
Kim SH
No affiliation info available
Chang IA
No affiliation info available
Jeon SH
No affiliation info available
Park BJ
No affiliation info available
Song GJ
No affiliation info available
Lee MG
No affiliation info available
Kim IS
No affiliation info available
Kim YW
No affiliation info available

MeSH

Antibodies, BacterialAntigens, BacterialEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, IndirectGoldHumansImmunoassayImmunoglobulin GImmunoglobulin MOrientia tsutsugamushiRecombinant ProteinsScrub TyphusSensitivity and SpecificitySerotyping

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23678257

Citation

Kim, Young-Jin, et al. "Improvement of the Diagnostic Sensitivity of Scrub Typhus Using a Mixture of Recombinant Antigens Derived From Orientia Tsutsugamushi Serotypes." Journal of Korean Medical Science, vol. 28, no. 5, 2013, pp. 672-9.
Kim YJ, Yeo SJ, Park SJ, et al. Improvement of the diagnostic sensitivity of scrub typhus using a mixture of recombinant antigens derived from Orientia tsutsugamushi serotypes. J Korean Med Sci. 2013;28(5):672-9.
Kim, Y. J., Yeo, S. J., Park, S. J., Woo, Y. J., Kim, M. W., Kim, S. H., Chang, I. A., Jeon, S. H., Park, B. J., Song, G. J., Lee, M. G., Kim, I. S., & Kim, Y. W. (2013). Improvement of the diagnostic sensitivity of scrub typhus using a mixture of recombinant antigens derived from Orientia tsutsugamushi serotypes. Journal of Korean Medical Science, 28(5), 672-9. https://doi.org/10.3346/jkms.2013.28.5.672
Kim YJ, et al. Improvement of the Diagnostic Sensitivity of Scrub Typhus Using a Mixture of Recombinant Antigens Derived From Orientia Tsutsugamushi Serotypes. J Korean Med Sci. 2013;28(5):672-9. PubMed PMID: 23678257.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improvement of the diagnostic sensitivity of scrub typhus using a mixture of recombinant antigens derived from Orientia tsutsugamushi serotypes. AU - Kim,Young-Jin, AU - Yeo,Seon-Ju, AU - Park,Sang-Jin, AU - Woo,Ye-Ju, AU - Kim,Min-Woo, AU - Kim,Seung-Han, AU - Chang,In-Ae, AU - Jeon,Sung-Ho, AU - Park,Byung-Jae, AU - Song,Gi-Jun, AU - Lee,Myoung-Gu, AU - Kim,Ik-Sang, AU - Kim,Yoon-Won, Y1 - 2013/05/02/ PY - 2013/02/14/received PY - 2013/04/03/accepted PY - 2013/5/17/entrez PY - 2013/5/17/pubmed PY - 2013/12/16/medline KW - ELISA KW - IFA KW - Orientia tsutsugamushi KW - RDT KW - Recombinant Antigen KW - Scrub Typhus SP - 672 EP - 9 JF - Journal of Korean medical science JO - J Korean Med Sci VL - 28 IS - 5 N2 - Diagnosis of scrub typhus is difficult because its symptoms are very similar to other acute febrile illnesses, such as leptospirosis, murine typhus, and other viral hemorrhagic fevers. To differentiate scrub typhus from other acute febrile diseases, a rapid and reliable serological diagnosis is important. We have developed a chimeric recombinant antigen cr56 and two other recombinant antigens, r21 and kr56, from various serotypes of Orientia tsutsugamushi. They were tested for the detection of antibodies against O. tsutsugamushi in the patient's serum samples using enzyme-linked immunosorbent assay (ELISA) and dot-blot analyses. As of conventional immunofluorescence assay (IFA), when the mixture of these three recombinant antigens was used, both sensitivity and specificity of the recombinant antigens were increased up to 98% in IgM and IgG at ELISA and dot blotting. Additionally, both sensitivity and specificity by detection of IgM and IgG antibodies at rapid diagnostic test (RDT), using the mixture of three antigens and gold conjugated antibodies, were 99%. Our results suggest the use of mixture of these recombinant antigen proteins in ELISA or RDT is suitable as a diagnostic test for scrub typhus. SN - 1598-6357 UR - https://www.unboundmedicine.com/medline/citation/23678257/Improvement_of_the_diagnostic_sensitivity_of_scrub_typhus_using_a_mixture_of_recombinant_antigens_derived_from_Orientia_tsutsugamushi_serotypes_ L2 - https://jkms.org/DOIx.php?id=10.3346/jkms.2013.28.5.672 DB - PRIME DP - Unbound Medicine ER -