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4-Hydroxyderricin and xanthoangelol from Ashitaba (Angelica keiskei) suppress differentiation of preadiopocytes to adipocytes via AMPK and MAPK pathways.
Mol Nutr Food Res. 2013 Oct; 57(10):1729-40.MN

Abstract

SCOPE

Adipocytes differentiation is deeply involved in the onset of obesity. 4-Hydroxyderricin (4HD) and xanthoangelol (XAG) are the chalcones that are derived from Ashitaba (Angelica keiskei). In this study, we demonstrated the inhibitory effects of these chalcones on adipocytes differentiation.

METHODS AND RESULTS

4HD and XAG suppressed intracellular lipid accumulation by Oil red O staining at 5 μM without cytotoxicity. They inhibited adipocytes differentiation accompanied by down-expression of adipocyte-specific transcription factors, CCAAT/enhancer-binding protein-β (C/EBP-β), C/EBP-α, and peroxisome proliferator-activated receptor gamma (PPAR-γ) using RT-PCR and Western blotting analysis. To obtain insights into the underlying mechanism, the activation of AMP-activated protein kinase (AMPK) and mitogen-activated protein kinase pathways was investigated. These two chalcones promoted phosphorylation of AMPK and acetyl CoA carboxylase during differentiation of 3T3-L1 adipocytes accompanied by a decrease in glycerol-3-phosphate acyl transferase-1 and an increase in carnitine palmitoyltransferase-1 mRNA expression. These chalcones also promoted phosphorylation of extracellular signal-regulated kinases and Jun aminoterminal kinases, but not p38. Moreover, the inhibitors for AMPK and extracellular signal-regulated kinases abolished the chalcones-caused down-expression of C/EBP-β, C/EBP-α, and PPAR-γ. Treatment with Jun aminoterminal kinases inhibitor abolished the down-expression of C/EBP-α and PPAR-γ, but not C/EBP-β.

CONCLUSION

4HD and XAG inhibit adipocytes differentiation through AMPK and mitogen-activated protein kinase pathways, resulting in the down-expression of adipocyte-specific transcription factors.

Authors+Show Affiliations

Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23681764

Citation

Zhang, Tianshun, et al. "4-Hydroxyderricin and Xanthoangelol From Ashitaba (Angelica Keiskei) Suppress Differentiation of Preadiopocytes to Adipocytes Via AMPK and MAPK Pathways." Molecular Nutrition & Food Research, vol. 57, no. 10, 2013, pp. 1729-40.
Zhang T, Sawada K, Yamamoto N, et al. 4-Hydroxyderricin and xanthoangelol from Ashitaba (Angelica keiskei) suppress differentiation of preadiopocytes to adipocytes via AMPK and MAPK pathways. Mol Nutr Food Res. 2013;57(10):1729-40.
Zhang, T., Sawada, K., Yamamoto, N., & Ashida, H. (2013). 4-Hydroxyderricin and xanthoangelol from Ashitaba (Angelica keiskei) suppress differentiation of preadiopocytes to adipocytes via AMPK and MAPK pathways. Molecular Nutrition & Food Research, 57(10), 1729-40. https://doi.org/10.1002/mnfr.201300020
Zhang T, et al. 4-Hydroxyderricin and Xanthoangelol From Ashitaba (Angelica Keiskei) Suppress Differentiation of Preadiopocytes to Adipocytes Via AMPK and MAPK Pathways. Mol Nutr Food Res. 2013;57(10):1729-40. PubMed PMID: 23681764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 4-Hydroxyderricin and xanthoangelol from Ashitaba (Angelica keiskei) suppress differentiation of preadiopocytes to adipocytes via AMPK and MAPK pathways. AU - Zhang,Tianshun, AU - Sawada,Keisuke, AU - Yamamoto,Norio, AU - Ashida,Hitoshi, Y1 - 2013/05/16/ PY - 2013/01/08/received PY - 2013/03/08/revised PY - 2013/03/12/accepted PY - 2013/5/18/entrez PY - 2013/5/18/pubmed PY - 2014/5/17/medline KW - AMP-activated protein kinase KW - Ashitaba chalcones KW - CCAAT/enhancer-binding proteins KW - Mitogen-activated protein kinases KW - Peroxisome proliferator-activated receptor gamma SP - 1729 EP - 40 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 57 IS - 10 N2 - SCOPE: Adipocytes differentiation is deeply involved in the onset of obesity. 4-Hydroxyderricin (4HD) and xanthoangelol (XAG) are the chalcones that are derived from Ashitaba (Angelica keiskei). In this study, we demonstrated the inhibitory effects of these chalcones on adipocytes differentiation. METHODS AND RESULTS: 4HD and XAG suppressed intracellular lipid accumulation by Oil red O staining at 5 μM without cytotoxicity. They inhibited adipocytes differentiation accompanied by down-expression of adipocyte-specific transcription factors, CCAAT/enhancer-binding protein-β (C/EBP-β), C/EBP-α, and peroxisome proliferator-activated receptor gamma (PPAR-γ) using RT-PCR and Western blotting analysis. To obtain insights into the underlying mechanism, the activation of AMP-activated protein kinase (AMPK) and mitogen-activated protein kinase pathways was investigated. These two chalcones promoted phosphorylation of AMPK and acetyl CoA carboxylase during differentiation of 3T3-L1 adipocytes accompanied by a decrease in glycerol-3-phosphate acyl transferase-1 and an increase in carnitine palmitoyltransferase-1 mRNA expression. These chalcones also promoted phosphorylation of extracellular signal-regulated kinases and Jun aminoterminal kinases, but not p38. Moreover, the inhibitors for AMPK and extracellular signal-regulated kinases abolished the chalcones-caused down-expression of C/EBP-β, C/EBP-α, and PPAR-γ. Treatment with Jun aminoterminal kinases inhibitor abolished the down-expression of C/EBP-α and PPAR-γ, but not C/EBP-β. CONCLUSION: 4HD and XAG inhibit adipocytes differentiation through AMPK and mitogen-activated protein kinase pathways, resulting in the down-expression of adipocyte-specific transcription factors. SN - 1613-4133 UR - https://www.unboundmedicine.com/medline/citation/23681764/4_Hydroxyderricin_and_xanthoangelol_from_Ashitaba__Angelica_keiskei__suppress_differentiation_of_preadiopocytes_to_adipocytes_via_AMPK_and_MAPK_pathways_ L2 - https://doi.org/10.1002/mnfr.201300020 DB - PRIME DP - Unbound Medicine ER -