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Optogenetic strategies to investigate neural circuitry engaged by stress.
Behav Brain Res 2013; 255:19-25BB

Abstract

Optogenetic techniques have given researchers unprecedented access to the function of discrete neural circuit elements and have been instrumental in the identification of novel brain pathways that become dysregulated in neuropsychiatric diseases. For example, stress is integrally linked to the manifestation and pathophysiology of neuropsychiatric illness, including anxiety, addiction and depression. Due to the heterogeneous populations of genetically and neurochemically distinct neurons in areas such as the bed nucleus of the stria terminalis (BNST), as well as their substantial number of projections, our understanding of how neural circuits become disturbed after stress has been limited. Using optogenetic tools, we are now able to selectively isolate distinct neural circuits that contribute to these disorders and perturb these circuits in vivo, which in turn may lead to the normalization of maladaptive behavior. This review will focus on current optogenetic strategies to identify, manipulate, and record from discrete neural circuit elements in vivo as well as highlight recent optogenetic studies that have been utilized to parcel out BNST function.

Authors+Show Affiliations

Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Bowles Center for Alcohol Studies, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Electronic address: dennis_sparta@med.unc.edu.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

23684554

Citation

Sparta, Dennis R., et al. "Optogenetic Strategies to Investigate Neural Circuitry Engaged By Stress." Behavioural Brain Research, vol. 255, 2013, pp. 19-25.
Sparta DR, Jennings JH, Ung RL, et al. Optogenetic strategies to investigate neural circuitry engaged by stress. Behav Brain Res. 2013;255:19-25.
Sparta, D. R., Jennings, J. H., Ung, R. L., & Stuber, G. D. (2013). Optogenetic strategies to investigate neural circuitry engaged by stress. Behavioural Brain Research, 255, pp. 19-25. doi:10.1016/j.bbr.2013.05.007.
Sparta DR, et al. Optogenetic Strategies to Investigate Neural Circuitry Engaged By Stress. Behav Brain Res. 2013 Oct 15;255:19-25. PubMed PMID: 23684554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optogenetic strategies to investigate neural circuitry engaged by stress. AU - Sparta,Dennis R, AU - Jennings,Joshua H, AU - Ung,Randall L, AU - Stuber,Garret D, Y1 - 2013/05/16/ PY - 2013/02/01/received PY - 2013/04/24/revised PY - 2013/05/06/accepted PY - 2013/5/21/entrez PY - 2013/5/21/pubmed PY - 2014/10/28/medline KW - AAV KW - AGRP KW - ARC KW - Arch KW - BLA KW - BNST KW - CRF KW - CaMKIIα KW - CeA KW - ChR2 KW - Circuit mapping KW - DAT KW - GABA KW - HSV KW - LDT KW - LED KW - LH KW - LHb KW - MeA KW - NAc KW - NPY KW - NpHR KW - Optogenetics KW - PAG KW - PBN KW - POMC KW - PRV KW - PTSD KW - PVH KW - RG KW - RMTg KW - SNr KW - Stress KW - TH KW - TTX KW - TVA KW - Vgat KW - Vglut KW - W KW - adeno-associated virus KW - agouti-related peptide KW - archaerhodopsin KW - arcuate nucleus of the hypothalamus KW - avian retroviral receptor KW - basolateral amygdala KW - bed nucleus of the stria terminalis KW - calcium–calmodulin dependent protein kinase IIα KW - central nucleus of the amygdala KW - channelrhodopsin-2 KW - corticotropin releasing factor KW - dopamine transporter KW - halorhodopsin KW - herpes simplex virus KW - lateral habenula KW - lateral hypothalamus KW - laterodorsal tegmentum KW - light emitting diode KW - medial nucleus of the amygdala KW - neuropeptide Y KW - nucleus accumbens KW - parabrachial nucleus KW - paraventricular nucleus of the hypothalamus KW - periaqueductal gray KW - post traumatic stress disorder KW - pro-opiomelanocortin KW - pseudorabies virus KW - rabies virus envelop glycoprotein KW - rostromedial tegmental nucleus KW - substantia nigra reticulata KW - tetrodotoxin KW - tyrosine hydroxylase KW - vesicular GABA transporter KW - vesicular glutamate transporters KW - watts KW - γ-Aminobutyric acid SP - 19 EP - 25 JF - Behavioural brain research JO - Behav. Brain Res. VL - 255 N2 - Optogenetic techniques have given researchers unprecedented access to the function of discrete neural circuit elements and have been instrumental in the identification of novel brain pathways that become dysregulated in neuropsychiatric diseases. For example, stress is integrally linked to the manifestation and pathophysiology of neuropsychiatric illness, including anxiety, addiction and depression. Due to the heterogeneous populations of genetically and neurochemically distinct neurons in areas such as the bed nucleus of the stria terminalis (BNST), as well as their substantial number of projections, our understanding of how neural circuits become disturbed after stress has been limited. Using optogenetic tools, we are now able to selectively isolate distinct neural circuits that contribute to these disorders and perturb these circuits in vivo, which in turn may lead to the normalization of maladaptive behavior. This review will focus on current optogenetic strategies to identify, manipulate, and record from discrete neural circuit elements in vivo as well as highlight recent optogenetic studies that have been utilized to parcel out BNST function. SN - 1872-7549 UR - https://www.unboundmedicine.com/medline/citation/23684554/Optogenetic_strategies_to_investigate_neural_circuitry_engaged_by_stress_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-4328(13)00268-4 DB - PRIME DP - Unbound Medicine ER -