Tags

Type your tag names separated by a space and hit enter

A new approach for the carbon monoxide (CO) exposure diagnosis: measurement of total CO in human blood versus carboxyhemoglobin (HbCO).
J Forensic Sci. 2013 Jul; 58(4):1041-6.JF

Abstract

The aim of the study is to present the application of a headspace-gas chromatography-mass spectrometry (HS-GC-MS) method for the determination of the carbon monoxide (CO) blood concentration and to compare it with carboxyhemoglobin (HbCO) saturation. In postmortem cases, the HbCO measured by spectrophotometry frequently leads to inaccurate results due to inadequate samples or analyses. The true role of CO intoxication in the death of a person could be misclassified. The estimation of HbCO from HS-GC-MS CO measurements provides helpful information by determining the total CO levels (CO linked to hemoglobin (HbCO) and CO dissociated from hemoglobin). The CO concentrations were converted in HbCO saturation levels to define cutoff blood CO values. CO limits were defined as less than 1 μmol/mL for living persons, less than 1.5 μmol/mL for dead persons without CO exposure, and greater than 3 μmol/mL for dead persons with clear CO poisoning.

Links

Aggregator Full Text

Authors+Show Affiliations

Varlet V
University Center of Legal Medicine Lausanne - Geneva, Forensic Toxicology and Chemistry Unit, CH-1011, Lausanne, Switzerland. vincent.varlet@chuv.ch
De Croutte EL
No affiliation info available
Augsburger M
No affiliation info available
Mangin P
No affiliation info available

MeSH

AdultAgedCarbon MonoxideCarbon Monoxide PoisoningCarboxyhemoglobinFemaleForensic ToxicologyGas Chromatography-Mass SpectrometryHumansMaleMiddle AgedModels, BiologicalSpectrophotometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23692308

Citation

Varlet, Vincent, et al. "A New Approach for the Carbon Monoxide (CO) Exposure Diagnosis: Measurement of Total CO in Human Blood Versus Carboxyhemoglobin (HbCO)." Journal of Forensic Sciences, vol. 58, no. 4, 2013, pp. 1041-6.
Varlet V, De Croutte EL, Augsburger M, et al. A new approach for the carbon monoxide (CO) exposure diagnosis: measurement of total CO in human blood versus carboxyhemoglobin (HbCO). J Forensic Sci. 2013;58(4):1041-6.
Varlet, V., De Croutte, E. L., Augsburger, M., & Mangin, P. (2013). A new approach for the carbon monoxide (CO) exposure diagnosis: measurement of total CO in human blood versus carboxyhemoglobin (HbCO). Journal of Forensic Sciences, 58(4), 1041-6. https://doi.org/10.1111/1556-4029.12130
Varlet V, et al. A New Approach for the Carbon Monoxide (CO) Exposure Diagnosis: Measurement of Total CO in Human Blood Versus Carboxyhemoglobin (HbCO). J Forensic Sci. 2013;58(4):1041-6. PubMed PMID: 23692308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new approach for the carbon monoxide (CO) exposure diagnosis: measurement of total CO in human blood versus carboxyhemoglobin (HbCO). AU - Varlet,Vincent, AU - De Croutte,Emma Lagroy, AU - Augsburger,Marc, AU - Mangin,Patrice, Y1 - 2013/05/21/ PY - 2012/01/25/received PY - 2012/05/02/revised PY - 2012/05/12/accepted PY - 2013/5/23/entrez PY - 2013/5/23/pubmed PY - 2014/3/29/medline KW - carbon monoxide KW - carboxyhemoglobin KW - forensic science KW - headspace gas extraction KW - postmortem blood KW - putrefaction SP - 1041 EP - 6 JF - Journal of forensic sciences JO - J Forensic Sci VL - 58 IS - 4 N2 - The aim of the study is to present the application of a headspace-gas chromatography-mass spectrometry (HS-GC-MS) method for the determination of the carbon monoxide (CO) blood concentration and to compare it with carboxyhemoglobin (HbCO) saturation. In postmortem cases, the HbCO measured by spectrophotometry frequently leads to inaccurate results due to inadequate samples or analyses. The true role of CO intoxication in the death of a person could be misclassified. The estimation of HbCO from HS-GC-MS CO measurements provides helpful information by determining the total CO levels (CO linked to hemoglobin (HbCO) and CO dissociated from hemoglobin). The CO concentrations were converted in HbCO saturation levels to define cutoff blood CO values. CO limits were defined as less than 1 μmol/mL for living persons, less than 1.5 μmol/mL for dead persons without CO exposure, and greater than 3 μmol/mL for dead persons with clear CO poisoning. SN - 1556-4029 UR - https://www.unboundmedicine.com/medline/citation/23692308/A_new_approach_for_the_carbon_monoxide__CO__exposure_diagnosis:_measurement_of_total_CO_in_human_blood_versus_carboxyhemoglobin__HbCO__ L2 - https://doi.org/10.1111/1556-4029.12130 DB - PRIME DP - Unbound Medicine ER -