TY - JOUR T1 - Full-genome deep sequencing and phylogenetic analysis of novel human betacoronavirus. AU - Cotten,Matthew, AU - Lam,Tommy T, AU - Watson,Simon J, AU - Palser,Anne L, AU - Petrova,Velislava, AU - Grant,Paul, AU - Pybus,Oliver G, AU - Rambaut,Andrew, AU - Guan,Yi, AU - Pillay,Deenan, AU - Kellam,Paul, AU - Nastouli,Eleni, PY - 2013/5/23/entrez PY - 2013/5/23/pubmed PY - 2013/10/30/medline KW - computer-aided design KW - coronavirus infections KW - disease transmission KW - evolution KW - high-throughput nucleotide sequencing KW - infectious KW - molecular KW - respiratory tract infections KW - viruses KW - zoonoses SP - 736 EP - 42B JF - Emerging infectious diseases JO - Emerg Infect Dis VL - 19 IS - 5 N2 - A novel betacoronavirus associated with lethal respiratory and renal complications was recently identified in patients from several countries in the Middle East. We report the deep genome sequencing of the virus directly from a patient's sputum sample. Our high-throughput sequencing yielded a substantial depth of genome sequence assembly and showed the minority viral variants in the specimen. Detailed phylogenetic analysis of the virus genome (England/Qatar/2012) revealed its close relationship to European bat coronaviruses circulating among the bat species of the Vespertilionidae family. Molecular clock analysis showed that the 2 human infections of this betacoronavirus in June 2012 (EMC/2012) and September 2012 (England/Qatar/2012) share a common virus ancestor most likely considerably before early 2012, suggesting the human diversity is the result of multiple zoonotic events. SN - 1080-6059 UR - https://www.unboundmedicine.com/medline/citation/23693015/Full_genome_deep_sequencing_and_phylogenetic_analysis_of_novel_human_betacoronavirus_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23693015/ DB - PRIME DP - Unbound Medicine ER -