Tags

Type your tag names separated by a space and hit enter

Compounded PHOSPHO1/ALPL deficiencies reduce dentin mineralization.
J Dent Res 2013; 92(8):721-7JD

Abstract

Phosphatases are involved in bone and tooth mineralization, but their mechanisms of action are not completely understood. Tissue-nonspecific alkaline phosphatase (TNAP, ALPL) regulates inhibitory extracellular pyrophosphate through its pyrophosphatase activity to control mineral propagation in the matrix; mice without TNAP lack acellular cementum, and have mineralization defects in dentin, enamel, and bone. PHOSPHO1 is a phosphatase found within membrane-bounded matrix vesicles in mineralized tissues, and double ablation of Alpl and Phospho1 in mice leads to a complete absence of skeletal mineralization. Here, we describe mineralization abnormalities in the teeth of Phospho1(-/-) mice, and in compound knockout mice lacking Phospho1 and one allele of Alpl (Phospho1(-/-);Alpl(+/-)). In wild-type mice, PHOSPHO1 and TNAP co-localized to odontoblasts at early stages of dentinogenesis, coincident with the early mineralization of mantle dentin. In Phospho1 knockout mice, radiography, micro-computed tomography, histology, and transmission electron microscopy all demonstrated mineralization abnormalities of incisor dentin, with the most remarkable findings being reduced overall mineralization coincident with decreased matrix vesicle mineralization in the Phospho1(-/-) mice, and the almost complete absence of matrix vesicles in the Phospho1(-/-);Alpl(+/-) mice, whose incisors showed a further reduction in mineralization. Results from this study support prominent non-redundant roles for both PHOSPHO1 and TNAP in dentin mineralization.

Authors+Show Affiliations

Faculty of Dentistry, and Department of Anatomy and Cell Biology, Faculty of Medicine, McGill University, 3640 University Street, Montreal, QC, Canada. marc.mckee@mcgill.caNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23694930

Citation

McKee, M D., et al. "Compounded PHOSPHO1/ALPL Deficiencies Reduce Dentin Mineralization." Journal of Dental Research, vol. 92, no. 8, 2013, pp. 721-7.
McKee MD, Yadav MC, Foster BL, et al. Compounded PHOSPHO1/ALPL deficiencies reduce dentin mineralization. J Dent Res. 2013;92(8):721-7.
McKee, M. D., Yadav, M. C., Foster, B. L., Somerman, M. J., Farquharson, C., & Millán, J. L. (2013). Compounded PHOSPHO1/ALPL deficiencies reduce dentin mineralization. Journal of Dental Research, 92(8), pp. 721-7. doi:10.1177/0022034513490958.
McKee MD, et al. Compounded PHOSPHO1/ALPL Deficiencies Reduce Dentin Mineralization. J Dent Res. 2013;92(8):721-7. PubMed PMID: 23694930.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Compounded PHOSPHO1/ALPL deficiencies reduce dentin mineralization. AU - McKee,M D, AU - Yadav,M C, AU - Foster,B L, AU - Somerman,M J, AU - Farquharson,C, AU - Millán,J L, Y1 - 2013/05/21/ PY - 2013/5/23/entrez PY - 2013/5/23/pubmed PY - 2013/9/21/medline KW - extracellular matrix KW - extracellular matrix proteins KW - matrix vesicles KW - phosphatase KW - tissue-non-specific alkaline phosphatase KW - tooth SP - 721 EP - 7 JF - Journal of dental research JO - J. Dent. Res. VL - 92 IS - 8 N2 - Phosphatases are involved in bone and tooth mineralization, but their mechanisms of action are not completely understood. Tissue-nonspecific alkaline phosphatase (TNAP, ALPL) regulates inhibitory extracellular pyrophosphate through its pyrophosphatase activity to control mineral propagation in the matrix; mice without TNAP lack acellular cementum, and have mineralization defects in dentin, enamel, and bone. PHOSPHO1 is a phosphatase found within membrane-bounded matrix vesicles in mineralized tissues, and double ablation of Alpl and Phospho1 in mice leads to a complete absence of skeletal mineralization. Here, we describe mineralization abnormalities in the teeth of Phospho1(-/-) mice, and in compound knockout mice lacking Phospho1 and one allele of Alpl (Phospho1(-/-);Alpl(+/-)). In wild-type mice, PHOSPHO1 and TNAP co-localized to odontoblasts at early stages of dentinogenesis, coincident with the early mineralization of mantle dentin. In Phospho1 knockout mice, radiography, micro-computed tomography, histology, and transmission electron microscopy all demonstrated mineralization abnormalities of incisor dentin, with the most remarkable findings being reduced overall mineralization coincident with decreased matrix vesicle mineralization in the Phospho1(-/-) mice, and the almost complete absence of matrix vesicles in the Phospho1(-/-);Alpl(+/-) mice, whose incisors showed a further reduction in mineralization. Results from this study support prominent non-redundant roles for both PHOSPHO1 and TNAP in dentin mineralization. SN - 1544-0591 UR - https://www.unboundmedicine.com/medline/citation/23694930/Compounded_PHOSPHO1/ALPL_deficiencies_reduce_dentin_mineralization_ L2 - http://journals.sagepub.com/doi/full/10.1177/0022034513490958?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -