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NK cells are necessary for recovery of corneal CD11c+ dendritic cells after epithelial abrasion injury.
J Leukoc Biol. 2013 Aug; 94(2):343-51.JL

Abstract

Mechanisms controlling CD11c(+) MHCII(+) DCs during corneal epithelial wound healing were investigated in a murine model of corneal abrasion. Selective depletion of NKp46(+) CD3- NK cells that normally migrate into the cornea after epithelial abrasion resulted in >85% reduction of the epithelial CD11c(+) MHCII(+) DCs, normally present during and after epithelial wound closure. Transfer (i.v.) of spleen NK cells into NK cell-depleted mice significantly restored levels of corneal epithelial DCs (P<0.01). Immigrated NK cells were predominately positive for IFN-γ, and topical corneal anti-IFN-γ reduced epithelial DCs by 79% (P<0.01). IFN-γ(-/-) mice had 69% fewer DCs than WT controls (P<0.01), and topical rIFN-γ applied to NK cell-depleted corneas increased epithelial DCs significantly (P<0.01). The contribution of ICAM-1, an adhesion molecule involved in leukocyte migration, expressed on healing corneal epithelium, was evaluated. ICAM-1(-/-) mice exhibited >70% reduction in epithelial DC recovery in the first 48 h after epithelial abrasion (P<0.01). These interventions reveal an early turnover of DCs in the epithelium after injury, and ICAM-1, NK cells, and IFN-γ are necessary for the immigration phase of this turnover.

Authors+Show Affiliations

Clinical College of Ophthalmology, Tianjin Medical University, Tianjin Eye Hospital, Tianjin, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23695308

Citation

Gao, Yuan, et al. "NK Cells Are Necessary for Recovery of Corneal CD11c+ Dendritic Cells After Epithelial Abrasion Injury." Journal of Leukocyte Biology, vol. 94, no. 2, 2013, pp. 343-51.
Gao Y, Li Z, Hassan N, et al. NK cells are necessary for recovery of corneal CD11c+ dendritic cells after epithelial abrasion injury. J Leukoc Biol. 2013;94(2):343-51.
Gao, Y., Li, Z., Hassan, N., Mehta, P., Burns, A. R., Tang, X., & Smith, C. W. (2013). NK cells are necessary for recovery of corneal CD11c+ dendritic cells after epithelial abrasion injury. Journal of Leukocyte Biology, 94(2), 343-51. https://doi.org/10.1189/jlb.1212633
Gao Y, et al. NK Cells Are Necessary for Recovery of Corneal CD11c+ Dendritic Cells After Epithelial Abrasion Injury. J Leukoc Biol. 2013;94(2):343-51. PubMed PMID: 23695308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NK cells are necessary for recovery of corneal CD11c+ dendritic cells after epithelial abrasion injury. AU - Gao,Yuan, AU - Li,Zhijie, AU - Hassan,Nida, AU - Mehta,Pooja, AU - Burns,Alan R, AU - Tang,Xin, AU - Smith,C Wayne, Y1 - 2013/05/21/ PY - 2013/5/23/entrez PY - 2013/5/23/pubmed PY - 2013/10/18/medline KW - ICAM-1 KW - IFN-γ KW - cornea KW - healing SP - 343 EP - 51 JF - Journal of leukocyte biology JO - J Leukoc Biol VL - 94 IS - 2 N2 - Mechanisms controlling CD11c(+) MHCII(+) DCs during corneal epithelial wound healing were investigated in a murine model of corneal abrasion. Selective depletion of NKp46(+) CD3- NK cells that normally migrate into the cornea after epithelial abrasion resulted in >85% reduction of the epithelial CD11c(+) MHCII(+) DCs, normally present during and after epithelial wound closure. Transfer (i.v.) of spleen NK cells into NK cell-depleted mice significantly restored levels of corneal epithelial DCs (P<0.01). Immigrated NK cells were predominately positive for IFN-γ, and topical corneal anti-IFN-γ reduced epithelial DCs by 79% (P<0.01). IFN-γ(-/-) mice had 69% fewer DCs than WT controls (P<0.01), and topical rIFN-γ applied to NK cell-depleted corneas increased epithelial DCs significantly (P<0.01). The contribution of ICAM-1, an adhesion molecule involved in leukocyte migration, expressed on healing corneal epithelium, was evaluated. ICAM-1(-/-) mice exhibited >70% reduction in epithelial DC recovery in the first 48 h after epithelial abrasion (P<0.01). These interventions reveal an early turnover of DCs in the epithelium after injury, and ICAM-1, NK cells, and IFN-γ are necessary for the immigration phase of this turnover. SN - 1938-3673 UR - https://www.unboundmedicine.com/medline/citation/23695308/NK_cells_are_necessary_for_recovery_of_corneal_CD11c+_dendritic_cells_after_epithelial_abrasion_injury_ L2 - https://doi.org/10.1189/jlb.1212633 DB - PRIME DP - Unbound Medicine ER -