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Neuroprotective effect of EGb761® and low-dose whole-body γ-irradiation in a rat model of Parkinson's disease.
Toxicol Ind Health. 2015 Dec; 31(12):1128-43.TI

Abstract

Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease. The present study was undertaken to investigate the pretreatment effects of standardized Ginkgo biloba extract (EGb761(®)) and low-dose whole-body γ-irradiation on the neurological dysfunction in the reserpine model of PD. Male Wistar rats were pretreated orally with EGb761 or fractionated low-dose whole-body γ-irradiation or their combination, then subjected to intraperitoneal injection of reserpine (5 mg/kg body weight) 24 h after the final dose of EGb761 or radiation. Reserpine injection resulted in the depletion of striatal dopamine (DA) level, increased catalepsy score, increased oxidative stress indicated via depletion of glutathione (GSH), increased malondialdehyde (MDA) and iron levels, decreased DA metabolites metabolizing enzymes; indicated by inhibition by glutathione-S-transferase, and nicotinamide adenine dinucleotide phosphate (NADPH)-quinone oxidoreductase (NQO) activities, mitochondrial dysfunction; indicated by declined complex I activity, and adenosine triphosphate (ATP) level and increased apoptosis; indicated by decreased mitochondrial B cell lymphoma-2 (Bcl-2) protein level and by transmission electron microscope. EGb761 and low-dose γ-radiation ameliorated the reserpine-induced state of oxidative stress, mitochondrial dysfunction, and apoptosis in brain. It can be concluded that EGb761, a widely used herbal medicine and low dose of γ-irradiation have protective effects for combating Parkinsonism possibly via replenishment of GSH levels.

Authors+Show Affiliations

Drug Radiation Research Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt nerminsadik@yahoo.com.Drug Radiation Research Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23696346

Citation

El-Ghazaly, Mona A., et al. "Neuroprotective Effect of EGb761® and Low-dose Whole-body Γ-irradiation in a Rat Model of Parkinson's Disease." Toxicology and Industrial Health, vol. 31, no. 12, 2015, pp. 1128-43.
El-Ghazaly MA, Sadik NA, Rashed ER, et al. Neuroprotective effect of EGb761® and low-dose whole-body γ-irradiation in a rat model of Parkinson's disease. Toxicol Ind Health. 2015;31(12):1128-43.
El-Ghazaly, M. A., Sadik, N. A., Rashed, E. R., & Abd-El-Fattah, A. A. (2015). Neuroprotective effect of EGb761® and low-dose whole-body γ-irradiation in a rat model of Parkinson's disease. Toxicology and Industrial Health, 31(12), 1128-43. https://doi.org/10.1177/0748233713487251
El-Ghazaly MA, et al. Neuroprotective Effect of EGb761® and Low-dose Whole-body Γ-irradiation in a Rat Model of Parkinson's Disease. Toxicol Ind Health. 2015;31(12):1128-43. PubMed PMID: 23696346.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotective effect of EGb761® and low-dose whole-body γ-irradiation in a rat model of Parkinson's disease. AU - El-Ghazaly,Mona A, AU - Sadik,Nermin A H, AU - Rashed,Engy R, AU - Abd-El-Fattah,Amal A, Y1 - 2013/05/21/ PY - 2013/5/23/entrez PY - 2013/5/23/pubmed PY - 2016/9/17/medline KW - Ginkgo biloba KW - Parkinsonism KW - low dose whole-body γ-irradiation KW - rats KW - reserpine SP - 1128 EP - 43 JF - Toxicology and industrial health JO - Toxicol Ind Health VL - 31 IS - 12 N2 - Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease. The present study was undertaken to investigate the pretreatment effects of standardized Ginkgo biloba extract (EGb761(®)) and low-dose whole-body γ-irradiation on the neurological dysfunction in the reserpine model of PD. Male Wistar rats were pretreated orally with EGb761 or fractionated low-dose whole-body γ-irradiation or their combination, then subjected to intraperitoneal injection of reserpine (5 mg/kg body weight) 24 h after the final dose of EGb761 or radiation. Reserpine injection resulted in the depletion of striatal dopamine (DA) level, increased catalepsy score, increased oxidative stress indicated via depletion of glutathione (GSH), increased malondialdehyde (MDA) and iron levels, decreased DA metabolites metabolizing enzymes; indicated by inhibition by glutathione-S-transferase, and nicotinamide adenine dinucleotide phosphate (NADPH)-quinone oxidoreductase (NQO) activities, mitochondrial dysfunction; indicated by declined complex I activity, and adenosine triphosphate (ATP) level and increased apoptosis; indicated by decreased mitochondrial B cell lymphoma-2 (Bcl-2) protein level and by transmission electron microscope. EGb761 and low-dose γ-radiation ameliorated the reserpine-induced state of oxidative stress, mitochondrial dysfunction, and apoptosis in brain. It can be concluded that EGb761, a widely used herbal medicine and low dose of γ-irradiation have protective effects for combating Parkinsonism possibly via replenishment of GSH levels. SN - 1477-0393 UR - https://www.unboundmedicine.com/medline/citation/23696346/Neuroprotective_effect_of_EGb761®_and_low_dose_whole_body_γ_irradiation_in_a_rat_model_of_Parkinson's_disease_ L2 - http://journals.sagepub.com/doi/full/10.1177/0748233713487251?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -