Tags

Type your tag names separated by a space and hit enter

Histopathological study of murine pulmonary cryptococcosis induced by Cryptococcus gattii and Cryptococcus neoformans.
Jpn J Infect Dis. 2013; 66(3):216-21.JJ

Abstract

Although Cryptococcus gattii can cause life-threatening complications, putative virulence factors of C. gattii remain controversial. Therefore, we conducted the present study to elucidate the virulence factors of the yeast and found that the mortality rate of mice infected with C. gattii R265 was significantly higher than that of those infected with C. gattii 5815; however, no difference was found in the mortality rates between mice infected with C. gattii R265 and Cryptococcus neoformans H99. In contrast, we found a significant difference in histopathological findings of the lungs between mice infected with C. gattii R265 and C. neoformans H99. The former showed alveolar expansion due to yeast proliferation with much lesser macrophage response, whereas the latter showed numerous nodules in the alveolar space consisting of macrophages and multinucleated giant cells. Furthermore, alveolar expansion was more enhanced in mice infected with C. gattii R265 than in those infected with C. gattii 5815. Our study confirmed that there is a different pathophysiology leading to death during C. gattii and C. neoformans infections. The result can provide two characteristics of C. gattii: one includes some mechanisms to escape from host recognition via macrophage and another includes a high performance of pulmonary structural alteration. These characteristics may be associated with the high virulence of C. gattii.

Authors+Show Affiliations

Okubo Y
Department of Surgical Pathology, Toho University School of Medicine, Tokyo, Japan.
Wakayama M
No affiliation info available
Ohno H
No affiliation info available
Yamamoto S
No affiliation info available
Tochigi N
No affiliation info available
Tanabe K
No affiliation info available
Kaneko Y
No affiliation info available
Yamagoe S
No affiliation info available
Umeyama T
No affiliation info available
Shinozaki M
No affiliation info available
Nemoto T
No affiliation info available
Nakayama H
No affiliation info available
Sasai D
No affiliation info available
Ishiwatari T
No affiliation info available
Shimodaira K
No affiliation info available
Yamamoto Y
No affiliation info available
Kamei K
No affiliation info available
Miyazaki Y
No affiliation info available
Shibuya K
No affiliation info available

MeSH

AnimalsCryptococcosisCryptococcus gattiiCryptococcus neoformansDisease Models, AnimalFemaleHistocytochemistryLungLung Diseases, ParasiticMiceMice, Inbred C57BLSurvival AnalysisVirulence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23698482

Citation

Okubo, Yoichiro, et al. "Histopathological Study of Murine Pulmonary Cryptococcosis Induced By Cryptococcus Gattii and Cryptococcus Neoformans." Japanese Journal of Infectious Diseases, vol. 66, no. 3, 2013, pp. 216-21.
Okubo Y, Wakayama M, Ohno H, et al. Histopathological study of murine pulmonary cryptococcosis induced by Cryptococcus gattii and Cryptococcus neoformans. Jpn J Infect Dis. 2013;66(3):216-21.
Okubo, Y., Wakayama, M., Ohno, H., Yamamoto, S., Tochigi, N., Tanabe, K., Kaneko, Y., Yamagoe, S., Umeyama, T., Shinozaki, M., Nemoto, T., Nakayama, H., Sasai, D., Ishiwatari, T., Shimodaira, K., Yamamoto, Y., Kamei, K., Miyazaki, Y., & Shibuya, K. (2013). Histopathological study of murine pulmonary cryptococcosis induced by Cryptococcus gattii and Cryptococcus neoformans. Japanese Journal of Infectious Diseases, 66(3), 216-21.
Okubo Y, et al. Histopathological Study of Murine Pulmonary Cryptococcosis Induced By Cryptococcus Gattii and Cryptococcus Neoformans. Jpn J Infect Dis. 2013;66(3):216-21. PubMed PMID: 23698482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Histopathological study of murine pulmonary cryptococcosis induced by Cryptococcus gattii and Cryptococcus neoformans. AU - Okubo,Yoichiro, AU - Wakayama,Megumi, AU - Ohno,Hideaki, AU - Yamamoto,Shuhei, AU - Tochigi,Naobumi, AU - Tanabe,Koichi, AU - Kaneko,Yukihiro, AU - Yamagoe,Satoshi, AU - Umeyama,Takashi, AU - Shinozaki,Minoru, AU - Nemoto,Tetsuo, AU - Nakayama,Haruo, AU - Sasai,Daisuke, AU - Ishiwatari,Takao, AU - Shimodaira,Kayoko, AU - Yamamoto,Yoshiro, AU - Kamei,Katsuhiko, AU - Miyazaki,Yoshitsugu, AU - Shibuya,Kazutoshi, PY - 2013/5/24/entrez PY - 2013/5/24/pubmed PY - 2013/12/16/medline SP - 216 EP - 21 JF - Japanese journal of infectious diseases JO - Jpn J Infect Dis VL - 66 IS - 3 N2 - Although Cryptococcus gattii can cause life-threatening complications, putative virulence factors of C. gattii remain controversial. Therefore, we conducted the present study to elucidate the virulence factors of the yeast and found that the mortality rate of mice infected with C. gattii R265 was significantly higher than that of those infected with C. gattii 5815; however, no difference was found in the mortality rates between mice infected with C. gattii R265 and Cryptococcus neoformans H99. In contrast, we found a significant difference in histopathological findings of the lungs between mice infected with C. gattii R265 and C. neoformans H99. The former showed alveolar expansion due to yeast proliferation with much lesser macrophage response, whereas the latter showed numerous nodules in the alveolar space consisting of macrophages and multinucleated giant cells. Furthermore, alveolar expansion was more enhanced in mice infected with C. gattii R265 than in those infected with C. gattii 5815. Our study confirmed that there is a different pathophysiology leading to death during C. gattii and C. neoformans infections. The result can provide two characteristics of C. gattii: one includes some mechanisms to escape from host recognition via macrophage and another includes a high performance of pulmonary structural alteration. These characteristics may be associated with the high virulence of C. gattii. SN - 1884-2836 UR - https://www.unboundmedicine.com/medline/citation/23698482/Histopathological_study_of_murine_pulmonary_cryptococcosis_induced_by_Cryptococcus_gattii_and_Cryptococcus_neoformans_ DB - PRIME DP - Unbound Medicine ER -