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Diagnostic yield of heat shock protein 70 (HSP-70) and anti-HSP-70 in Behcet-induced uveitis.
Scand J Immunol. 2013 Jun; 77(6):476-81.SJ

Abstract

Heat shock proteins (HSPs) are intracellular proteins with pro- and anti-inflammatory actions, playing an important role in the pathogenesis of Behcet's disease (BD). Diagnosis of BD uveitis in early stages is still problematic, thus this study was undertaken to determine diagnostic values of serum HSP- and anti-HSP-70 in BD uveitis. Serum levels of HSP- and anti-HSP-70 were measured in 53 patients with BD (26 with and 27 without uveitis). In control group, 25 age- and sex-matched idiopathic uveitis patients were enrolled consecutively. Both groups had no medical problems save uveitis at the time of sampling. Confounders like medications were analysed subsequently. HSP- and anti-HSP-70 values were measured by commercial ELISA kits. Data were analysed by spss 11.5 and medcalc 11.5.1 software. The Mean HSP-70 serum levels were different among aforementioned subgroups (P = 0.001, anova). They were elevated in BD uveitis compared with BD without uveitis (4.84 ± 4.21 versus 2.24 ± 2.08 ng/ml; P = 0.045). HSP-70 in sera of BD uveitis was also higher than that parameter in patients with idiopathic uveitis (4.84 ± 4.21 versus 2.37 ± 3.30 ng/ml; P = 0.001; cut-off point value 1.0 9 ng/ml, 95% CI 0.61-0.86, P = 0.0002, β = 0.06). However, there was not any statistical difference among those groups in the serum anti-HSP-70 levels (P = 0.63, anova). Multiple regression analysis demonstrated that among different confounders, only prednisolone increases and BD uveitis decreases HSP-70 levels independently. This prospective cross-sectional study suggested that HSP-70 serum level is impressed over the course of BD uveitis, and it could be utilized to diagnose or predict developing it.

Authors+Show Affiliations

Rheumatic Diseases Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23701436

Citation

Sahebari, M, et al. "Diagnostic Yield of Heat Shock Protein 70 (HSP-70) and anti-HSP-70 in Behcet-induced Uveitis." Scandinavian Journal of Immunology, vol. 77, no. 6, 2013, pp. 476-81.
Sahebari M, Hashemzadeh K, Mahmoudi M, et al. Diagnostic yield of heat shock protein 70 (HSP-70) and anti-HSP-70 in Behcet-induced uveitis. Scand J Immunol. 2013;77(6):476-81.
Sahebari, M., Hashemzadeh, K., Mahmoudi, M., Saremi, Z., & Mirfeizi, Z. (2013). Diagnostic yield of heat shock protein 70 (HSP-70) and anti-HSP-70 in Behcet-induced uveitis. Scandinavian Journal of Immunology, 77(6), 476-81. https://doi.org/10.1111/sji.12045
Sahebari M, et al. Diagnostic Yield of Heat Shock Protein 70 (HSP-70) and anti-HSP-70 in Behcet-induced Uveitis. Scand J Immunol. 2013;77(6):476-81. PubMed PMID: 23701436.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnostic yield of heat shock protein 70 (HSP-70) and anti-HSP-70 in Behcet-induced uveitis. AU - Sahebari,M, AU - Hashemzadeh,K, AU - Mahmoudi,M, AU - Saremi,Z, AU - Mirfeizi,Z, PY - 2012/11/28/received PY - 2013/02/07/accepted PY - 2013/5/25/entrez PY - 2013/5/25/pubmed PY - 2013/7/19/medline SP - 476 EP - 81 JF - Scandinavian journal of immunology JO - Scand J Immunol VL - 77 IS - 6 N2 - Heat shock proteins (HSPs) are intracellular proteins with pro- and anti-inflammatory actions, playing an important role in the pathogenesis of Behcet's disease (BD). Diagnosis of BD uveitis in early stages is still problematic, thus this study was undertaken to determine diagnostic values of serum HSP- and anti-HSP-70 in BD uveitis. Serum levels of HSP- and anti-HSP-70 were measured in 53 patients with BD (26 with and 27 without uveitis). In control group, 25 age- and sex-matched idiopathic uveitis patients were enrolled consecutively. Both groups had no medical problems save uveitis at the time of sampling. Confounders like medications were analysed subsequently. HSP- and anti-HSP-70 values were measured by commercial ELISA kits. Data were analysed by spss 11.5 and medcalc 11.5.1 software. The Mean HSP-70 serum levels were different among aforementioned subgroups (P = 0.001, anova). They were elevated in BD uveitis compared with BD without uveitis (4.84 ± 4.21 versus 2.24 ± 2.08 ng/ml; P = 0.045). HSP-70 in sera of BD uveitis was also higher than that parameter in patients with idiopathic uveitis (4.84 ± 4.21 versus 2.37 ± 3.30 ng/ml; P = 0.001; cut-off point value 1.0 9 ng/ml, 95% CI 0.61-0.86, P = 0.0002, β = 0.06). However, there was not any statistical difference among those groups in the serum anti-HSP-70 levels (P = 0.63, anova). Multiple regression analysis demonstrated that among different confounders, only prednisolone increases and BD uveitis decreases HSP-70 levels independently. This prospective cross-sectional study suggested that HSP-70 serum level is impressed over the course of BD uveitis, and it could be utilized to diagnose or predict developing it. SN - 1365-3083 UR - https://www.unboundmedicine.com/medline/citation/23701436/Diagnostic_yield_of_heat_shock_protein_70__HSP_70__and_anti_HSP_70_in_Behcet_induced_uveitis_ L2 - https://doi.org/10.1111/sji.12045 DB - PRIME DP - Unbound Medicine ER -