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Performance of the OSOM Trichomonas Rapid Test for diagnosis of Trichomonas vaginalis infection among women in Mysore, India.
Sex Health. 2013 Aug; 10(4):320-4.SH

Abstract

Background Trichomonas vaginalis is the world's most common treatable sexually transmissible infection. Currently, wet mount microscopy and syndromic management based on vaginal discharge are the most widely used methods for diagnosing and treating trichomoniasis in resource-constrained settings. Wet mount microscopy requires equipment and trained technicians, who are in short supply. We examined the diagnostic accuracy of the OSOM Trichomonas Rapid Test for detecting T. vaginalis vaginal infection among women in Mysore, India.

METHODS

During July 2009-August 2010, 450 sexually active women over 18 years seeking care at an urban reproductive health clinic were enrolled in the study. Clinician-collected vaginal swabs were evaluated for trichomonads using wet mount microscopy, InPouch culture and the OSOM test.

RESULTS

Of the 418 samples included in the analyses, culture detected 68 (16.3%) positive samples, wet mount microscopy detected 56 of the culture-positive samples and four false positive samples. The OSOM test detected 60 of the culture-positive samples plus two false positive cases. Using the composite reference standard (CRS), defined as wet mount- or culture-positive, the sensitivities of wet mount, the OSOM test and culture were 83.3%, 86.1% and 94.4%, respectively. The positive and negative predictive values of the OSOM test were 100% and 97.1% respectively. The Cohen's kappa agreement between the OSOM test and the CRS was excellent (κ=0.94).

CONCLUSION

The OSOM test has high sensitivity, excellent specificity, and excellent positive and negative predictive value compared to a CRS. This simple test can improve screening and diagnosis of T. vaginalis infection in resource-constrained settings where microscopy and culture are unavailable.

Authors+Show Affiliations

Department of Epidemiology, Robert Stempel College of Public Health & Social Work, 11200 SW 8 Street, HLS 390W2, Miami, FL 33199, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23702272

Citation

Madhivanan, Purnima, et al. "Performance of the OSOM Trichomonas Rapid Test for Diagnosis of Trichomonas Vaginalis Infection Among Women in Mysore, India." Sexual Health, vol. 10, no. 4, 2013, pp. 320-4.
Madhivanan P, Li T, Trammell S, et al. Performance of the OSOM Trichomonas Rapid Test for diagnosis of Trichomonas vaginalis infection among women in Mysore, India. Sex Health. 2013;10(4):320-4.
Madhivanan, P., Li, T., Trammell, S., Desai, C., Srinivas, V., Arun, A., Klausner, J. D., & Krupp, K. (2013). Performance of the OSOM Trichomonas Rapid Test for diagnosis of Trichomonas vaginalis infection among women in Mysore, India. Sexual Health, 10(4), 320-4. https://doi.org/10.1071/SH13015
Madhivanan P, et al. Performance of the OSOM Trichomonas Rapid Test for Diagnosis of Trichomonas Vaginalis Infection Among Women in Mysore, India. Sex Health. 2013;10(4):320-4. PubMed PMID: 23702272.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Performance of the OSOM Trichomonas Rapid Test for diagnosis of Trichomonas vaginalis infection among women in Mysore, India. AU - Madhivanan,Purnima, AU - Li,Tan, AU - Trammell,Stephanie, AU - Desai,Chirayu, AU - Srinivas,Vijaya, AU - Arun,Anjali, AU - Klausner,Jeffrey D, AU - Krupp,Karl, PY - 2013/01/30/received PY - 2013/04/08/accepted PY - 2013/5/25/entrez PY - 2013/5/25/pubmed PY - 2016/4/24/medline SP - 320 EP - 4 JF - Sexual health JO - Sex Health VL - 10 IS - 4 N2 - UNLABELLED: Background Trichomonas vaginalis is the world's most common treatable sexually transmissible infection. Currently, wet mount microscopy and syndromic management based on vaginal discharge are the most widely used methods for diagnosing and treating trichomoniasis in resource-constrained settings. Wet mount microscopy requires equipment and trained technicians, who are in short supply. We examined the diagnostic accuracy of the OSOM Trichomonas Rapid Test for detecting T. vaginalis vaginal infection among women in Mysore, India. METHODS: During July 2009-August 2010, 450 sexually active women over 18 years seeking care at an urban reproductive health clinic were enrolled in the study. Clinician-collected vaginal swabs were evaluated for trichomonads using wet mount microscopy, InPouch culture and the OSOM test. RESULTS: Of the 418 samples included in the analyses, culture detected 68 (16.3%) positive samples, wet mount microscopy detected 56 of the culture-positive samples and four false positive samples. The OSOM test detected 60 of the culture-positive samples plus two false positive cases. Using the composite reference standard (CRS), defined as wet mount- or culture-positive, the sensitivities of wet mount, the OSOM test and culture were 83.3%, 86.1% and 94.4%, respectively. The positive and negative predictive values of the OSOM test were 100% and 97.1% respectively. The Cohen's kappa agreement between the OSOM test and the CRS was excellent (κ=0.94). CONCLUSION: The OSOM test has high sensitivity, excellent specificity, and excellent positive and negative predictive value compared to a CRS. This simple test can improve screening and diagnosis of T. vaginalis infection in resource-constrained settings where microscopy and culture are unavailable. SN - 1448-5028 UR - https://www.unboundmedicine.com/medline/citation/23702272/Performance_of_the_OSOM_Trichomonas_Rapid_Test_for_diagnosis_of_Trichomonas_vaginalis_infection_among_women_in_Mysore_India_ L2 - https://medlineplus.gov/trichomoniasis.html DB - PRIME DP - Unbound Medicine ER -