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Fas ligand(+) fallopian tube epithelium induces apoptosis in both Fas receptor(+) T lymphocytes and endometrial cells.
Fertil Steril. 2013 Aug; 100(2):550-60.e3.FS

Abstract

OBJECTIVE

To establish whether human fallopian tube (FT) epithelium can induce apoptosis in T lymphocytes and endometrial cells.

DESIGN

Laboratory-based study.

SETTING

Hospital.

PATIENT(S)

Women undergoing abdominal hysterectomy for FT samples, and women volunteers with and without endometriosis for endometrial biopsies.

INTERVENTION(S)

FT samples obtained at time of surgery performed in reproductive-aged women with normal menstrual cycles.

MAIN OUTCOME MEASURE(S)

T lymphocytes or endometrial cells coincubated with FT epithelial cells and assayed for apoptosis by DNA nick-end labeling and caspase-3 activity, with the presence of Fas ligand (FasL) and Fas receptor (FasR) assessed by indirect immunostaining.

RESULT(S)

The epithelium of the FT-induced apoptosis in T cells as well as in human endometrial cells. The mechanism probably involves the FasL/FasR system; accordingly, we observed FasL at the apical surface of the epithelium and in the stroma of the FT at all phases of the menstrual cycle except during the early proliferative phase. The endometrial samples from patients with endometriosis did not express FasR and were resistant to apoptosis.

CONCLUSION(S)

In both FasR(+) T lymphocytes and endometrial cells, FasL(+) FT cells induce apoptosis. Data suggest that the FT epithelium acts as a barrier to limit the influx of lymphocytes as well as endometrial cells ascending the tube. Failure of these regulatory mechanisms may be related to the development of endometriosis.

Authors+Show Affiliations

Department of Obstetrics and Gynecology and Laboratory of Reproductive Biology, Faculty of Medicine, Universidad de Los Andes, Santiago, Chile.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23706333

Citation

Illanes, Sebastian E., et al. "Fas Ligand(+) Fallopian Tube Epithelium Induces Apoptosis in Both Fas Receptor(+) T Lymphocytes and Endometrial Cells." Fertility and Sterility, vol. 100, no. 2, 2013, pp. 550-60.e3.
Illanes SE, Maisey K, Sandoval M, et al. Fas ligand(+) fallopian tube epithelium induces apoptosis in both Fas receptor(+) T lymphocytes and endometrial cells. Fertil Steril. 2013;100(2):550-60.e3.
Illanes, S. E., Maisey, K., Sandoval, M., Reyes, F. E., Figueroa-Gaete, C., Pérez-Sepúlveda, A., Busquets, M., González, P., & Imarai, M. (2013). Fas ligand(+) fallopian tube epithelium induces apoptosis in both Fas receptor(+) T lymphocytes and endometrial cells. Fertility and Sterility, 100(2), 550-e3. https://doi.org/10.1016/j.fertnstert.2013.04.013
Illanes SE, et al. Fas Ligand(+) Fallopian Tube Epithelium Induces Apoptosis in Both Fas Receptor(+) T Lymphocytes and Endometrial Cells. Fertil Steril. 2013;100(2):550-60.e3. PubMed PMID: 23706333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fas ligand(+) fallopian tube epithelium induces apoptosis in both Fas receptor(+) T lymphocytes and endometrial cells. AU - Illanes,Sebastian E, AU - Maisey,Kevin, AU - Sandoval,Marcelo, AU - Reyes,Felipe E, AU - Figueroa-Gaete,Claudio, AU - Pérez-Sepúlveda,Alejandra, AU - Busquets,Maritza, AU - González,Patricia, AU - Imarai,Mónica, Y1 - 2013/05/23/ PY - 2012/04/03/received PY - 2013/02/27/revised PY - 2013/04/08/accepted PY - 2013/5/28/entrez PY - 2013/5/28/pubmed PY - 2013/12/16/medline KW - Apoptosis KW - T lymphocytes KW - endometrial cells KW - endometriosis KW - fallopian epithelial cells SP - 550 EP - 60.e3 JF - Fertility and sterility JO - Fertil. Steril. VL - 100 IS - 2 N2 - OBJECTIVE: To establish whether human fallopian tube (FT) epithelium can induce apoptosis in T lymphocytes and endometrial cells. DESIGN: Laboratory-based study. SETTING: Hospital. PATIENT(S): Women undergoing abdominal hysterectomy for FT samples, and women volunteers with and without endometriosis for endometrial biopsies. INTERVENTION(S): FT samples obtained at time of surgery performed in reproductive-aged women with normal menstrual cycles. MAIN OUTCOME MEASURE(S): T lymphocytes or endometrial cells coincubated with FT epithelial cells and assayed for apoptosis by DNA nick-end labeling and caspase-3 activity, with the presence of Fas ligand (FasL) and Fas receptor (FasR) assessed by indirect immunostaining. RESULT(S): The epithelium of the FT-induced apoptosis in T cells as well as in human endometrial cells. The mechanism probably involves the FasL/FasR system; accordingly, we observed FasL at the apical surface of the epithelium and in the stroma of the FT at all phases of the menstrual cycle except during the early proliferative phase. The endometrial samples from patients with endometriosis did not express FasR and were resistant to apoptosis. CONCLUSION(S): In both FasR(+) T lymphocytes and endometrial cells, FasL(+) FT cells induce apoptosis. Data suggest that the FT epithelium acts as a barrier to limit the influx of lymphocytes as well as endometrial cells ascending the tube. Failure of these regulatory mechanisms may be related to the development of endometriosis. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/23706333/Fas_ligand_+__fallopian_tube_epithelium_induces_apoptosis_in_both_Fas_receptor_+__T_lymphocytes_and_endometrial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(13)00509-8 DB - PRIME DP - Unbound Medicine ER -