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Characteristics of reversible absorption-enhancing effect of sodium nitroprusside in rat small intestine.
Eur J Pharm Sci. 2013 Jul 16; 49(4):664-70.EJ

Abstract

Nitric oxide (NO) donors increase the permeability of water-soluble compounds with neither loss of cell viability nor lactate dehydrogenase release. In addition, the rectal absorption of insulin has been reported to be remarkably enhanced in the presence of NO donors such as 1-Hydroxy-3-(3-aminopropyl)-3-isopropyltriazene 2-oxide (NOC5) and N-Ethyl-2-(1-ethyl-2-hydroxy-2-nitrosohydrazino) ethanamine (NOC12). In this study, we examined the effect of sodium nitroprusside (SNP), which is used in clinical situations as a vasodilator, as a model NO donor on the ileal mucosa of rats. We used an in situ closed loop method in rat ileum to study changes in the permeability of fluorescein isothiocyanate dextran 4000 (FD-4) as a paracellular marker. The effect of SNP (1 and 10mg/kg) on the protein expression level of the claudin family was examined by Western blotting. The membrane permeation of FD-4 was increased but no mucosal lesion was observed upon the administration of SNP. Moreover, the protein expression level of the claudin family was not changed by the administration of SNP. When SNP was removed 2h after its administration, no significant change in the membrane permeation of FD-4 was observed. Moreover, no decrease of ileal membrane resistance or disruption of membrane structure was observed. The absorption-enhancing effect of SNP was associated with low injury and low toxicity. The reversibility of the effect of SNP was observed. Consequently, it was shown that SNP can be a useful absorption enhancer.

Authors+Show Affiliations

Department of Drug Absorption and Pharmacokinetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan. takizawa@toyaku.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23707468

Citation

Takizawa, Yusuke, et al. "Characteristics of Reversible Absorption-enhancing Effect of Sodium Nitroprusside in Rat Small Intestine." European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences, vol. 49, no. 4, 2013, pp. 664-70.
Takizawa Y, Kishimoto H, Kitazato T, et al. Characteristics of reversible absorption-enhancing effect of sodium nitroprusside in rat small intestine. Eur J Pharm Sci. 2013;49(4):664-70.
Takizawa, Y., Kishimoto, H., Kitazato, T., Ishizaka, H., Kamiya, N., Ito, Y., Tomita, M., & Hayashi, M. (2013). Characteristics of reversible absorption-enhancing effect of sodium nitroprusside in rat small intestine. European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences, 49(4), 664-70. https://doi.org/10.1016/j.ejps.2013.05.013
Takizawa Y, et al. Characteristics of Reversible Absorption-enhancing Effect of Sodium Nitroprusside in Rat Small Intestine. Eur J Pharm Sci. 2013 Jul 16;49(4):664-70. PubMed PMID: 23707468.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characteristics of reversible absorption-enhancing effect of sodium nitroprusside in rat small intestine. AU - Takizawa,Yusuke, AU - Kishimoto,Hisanao, AU - Kitazato,Takuya, AU - Ishizaka,Haruka, AU - Kamiya,Naomi, AU - Ito,Yasuhiko, AU - Tomita,Mikio, AU - Hayashi,Masahiro, Y1 - 2013/05/24/ PY - 2013/01/22/received PY - 2013/05/14/revised PY - 2013/05/14/accepted PY - 2013/5/28/entrez PY - 2013/5/28/pubmed PY - 2014/2/12/medline KW - Absorption enhancer KW - Claudin family KW - Nitric oxide KW - Paracellular marker KW - Sodium nitroprusside SP - 664 EP - 70 JF - European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences JO - Eur J Pharm Sci VL - 49 IS - 4 N2 - Nitric oxide (NO) donors increase the permeability of water-soluble compounds with neither loss of cell viability nor lactate dehydrogenase release. In addition, the rectal absorption of insulin has been reported to be remarkably enhanced in the presence of NO donors such as 1-Hydroxy-3-(3-aminopropyl)-3-isopropyltriazene 2-oxide (NOC5) and N-Ethyl-2-(1-ethyl-2-hydroxy-2-nitrosohydrazino) ethanamine (NOC12). In this study, we examined the effect of sodium nitroprusside (SNP), which is used in clinical situations as a vasodilator, as a model NO donor on the ileal mucosa of rats. We used an in situ closed loop method in rat ileum to study changes in the permeability of fluorescein isothiocyanate dextran 4000 (FD-4) as a paracellular marker. The effect of SNP (1 and 10mg/kg) on the protein expression level of the claudin family was examined by Western blotting. The membrane permeation of FD-4 was increased but no mucosal lesion was observed upon the administration of SNP. Moreover, the protein expression level of the claudin family was not changed by the administration of SNP. When SNP was removed 2h after its administration, no significant change in the membrane permeation of FD-4 was observed. Moreover, no decrease of ileal membrane resistance or disruption of membrane structure was observed. The absorption-enhancing effect of SNP was associated with low injury and low toxicity. The reversibility of the effect of SNP was observed. Consequently, it was shown that SNP can be a useful absorption enhancer. SN - 1879-0720 UR - https://www.unboundmedicine.com/medline/citation/23707468/Characteristics_of_reversible_absorption_enhancing_effect_of_sodium_nitroprusside_in_rat_small_intestine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-0987(13)00192-9 DB - PRIME DP - Unbound Medicine ER -