Tags

Type your tag names separated by a space and hit enter

Evodiamine induces apoptosis and inhibits metastasis in MDA‑MB-231 human breast cancer cells in vitro and in vivo.
Oncol Rep. 2013 Aug; 30(2):685-94.OR

Abstract

Breast cancer remains the leading cause of cancer-related deaths among women. Owing to high efficiency and low toxic effects, further exploration of natural compounds from Chinese herbal medicine may be an efficient approach for breast cancer drug discovery. In this study, we investigated the effects of evodiamine on the growth and metastasis of MDA-MB-231 human breast cancer cells in vitro and in vivo. In vitro, evodiamine inhibited cell migration and invasion abilities through downregulation of MMP-9, urokinase-type plasminogen activator (uPA) and uPAR expression. Evodiamine-induced G0/G1 arrest and apoptosis were associated with a decrease in Bcl-2, cyclin D1 and cyclin-dependent kinase 6 (CDK6) expression and an increase in Bax and p27Kip1 expression. Moreover, evodiamine regulated p-ERK and p-p38 MAPK expression. Evodiamine-induced apoptosis was enhanced by its combination with the extracellular signal-regulated kinase (ERK) inhibitor PD98059 or the p38 mitogen-activated protein kinase (p38 MAPK) inhibitor SB203580. Evodiamine-inhibited metastasis was partly blocked by combination with PD98059 or SB203580. In vivo, the administration of evodiamine (10 mg/kg) significantly reduced tumor growth and pulmonary metastasis. These results demonstrate that evodiamine possesses antitumor activities via inhibition of cell migration and invasion, arrest of the cell cycle and induction of cell apoptosis in MDA-MB-231 cells.

Authors+Show Affiliations

Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Pudong, Shanghai 201203, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23708383

Citation

Du, Jia, et al. "Evodiamine Induces Apoptosis and Inhibits Metastasis in MDA‑MB-231 Human Breast Cancer Cells in Vitro and in Vivo." Oncology Reports, vol. 30, no. 2, 2013, pp. 685-94.
Du J, Wang XF, Zhou QM, et al. Evodiamine induces apoptosis and inhibits metastasis in MDA‑MB-231 human breast cancer cells in vitro and in vivo. Oncol Rep. 2013;30(2):685-94.
Du, J., Wang, X. F., Zhou, Q. M., Zhang, T. L., Lu, Y. Y., Zhang, H., & Su, S. B. (2013). Evodiamine induces apoptosis and inhibits metastasis in MDA‑MB-231 human breast cancer cells in vitro and in vivo. Oncology Reports, 30(2), 685-94. https://doi.org/10.3892/or.2013.2498
Du J, et al. Evodiamine Induces Apoptosis and Inhibits Metastasis in MDA‑MB-231 Human Breast Cancer Cells in Vitro and in Vivo. Oncol Rep. 2013;30(2):685-94. PubMed PMID: 23708383.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evodiamine induces apoptosis and inhibits metastasis in MDA‑MB-231 human breast cancer cells in vitro and in vivo. AU - Du,Jia, AU - Wang,Xiu-Feng, AU - Zhou,Qian-Mei, AU - Zhang,Tian-Ling, AU - Lu,Yi-Yu, AU - Zhang,Hui, AU - Su,Shi-Bing, Y1 - 2013/05/27/ PY - 2013/01/23/received PY - 2013/04/16/accepted PY - 2013/5/28/entrez PY - 2013/5/28/pubmed PY - 2014/2/28/medline SP - 685 EP - 94 JF - Oncology reports JO - Oncol Rep VL - 30 IS - 2 N2 - Breast cancer remains the leading cause of cancer-related deaths among women. Owing to high efficiency and low toxic effects, further exploration of natural compounds from Chinese herbal medicine may be an efficient approach for breast cancer drug discovery. In this study, we investigated the effects of evodiamine on the growth and metastasis of MDA-MB-231 human breast cancer cells in vitro and in vivo. In vitro, evodiamine inhibited cell migration and invasion abilities through downregulation of MMP-9, urokinase-type plasminogen activator (uPA) and uPAR expression. Evodiamine-induced G0/G1 arrest and apoptosis were associated with a decrease in Bcl-2, cyclin D1 and cyclin-dependent kinase 6 (CDK6) expression and an increase in Bax and p27Kip1 expression. Moreover, evodiamine regulated p-ERK and p-p38 MAPK expression. Evodiamine-induced apoptosis was enhanced by its combination with the extracellular signal-regulated kinase (ERK) inhibitor PD98059 or the p38 mitogen-activated protein kinase (p38 MAPK) inhibitor SB203580. Evodiamine-inhibited metastasis was partly blocked by combination with PD98059 or SB203580. In vivo, the administration of evodiamine (10 mg/kg) significantly reduced tumor growth and pulmonary metastasis. These results demonstrate that evodiamine possesses antitumor activities via inhibition of cell migration and invasion, arrest of the cell cycle and induction of cell apoptosis in MDA-MB-231 cells. SN - 1791-2431 UR - https://www.unboundmedicine.com/medline/citation/23708383/Evodiamine_induces_apoptosis_and_inhibits_metastasis_in_MDA‑MB_231_human_breast_cancer_cells_in_vitro_and_in_vivo_ L2 - http://www.spandidos-publications.com/or/30/2/685 DB - PRIME DP - Unbound Medicine ER -