Tags

Type your tag names separated by a space and hit enter

Differential muscle regulatory factor gene expression between larval and adult myogenesis in the frog Xenopus laevis: adult myogenic cell-specific myf5 upregulation and its relation to the notochord suppression of adult muscle differentiation.
In Vitro Cell Dev Biol Anim. 2013 Aug; 49(7):524-36.VC

Abstract

During Xenopus laevis metamorphosis, larval-to-adult muscle conversion depends on the differential responses of adult and larval myogenic cells to thyroid hormone. Essential differences in cell growth, differentiation, and hormone-dependent life-or-death fate have been reported between cultured larval (tail) and adult (hindlimb) myogenic cells. A previous study revealed that tail notochord cells suppress terminal differentiation in adult (but not larval) myogenic cells. However, little is known about the differences in expression patterns of myogenic regulatory factors (MRF) and the satellite cell marker Pax7 between adult and larval myogenic cells. In the present study, we compared mRNA expression of these factors between the two types. At first, reverse transcription polymerase chain reaction analysis of hindlimb buds showed sequential upregulation of myf5, myogenin, myod, and mrf4 during stages 50-54, when limb buds elongate and muscles begin to form. By contrast, in the tail, there was no such increase during the same period. Secondary, these results were duplicated in vitro: adult myogenic cells upregulated myf5, myod, and pax7 in the early culture period, followed by myogenin upregulation and myotube differentiation, while larval myogenic cells did not upregulate these genes and precociously started myotube differentiation. Thirdly, myf5 upregulation and early-phase proliferation in adult myogenic cells were potently inhibited by the presence of notochord cells, suggesting that notochord cells suppress adult myogenesis through inhibiting the transition from Myf5(-) stem cells to Myf5(+) committed myoblasts. All of the data presented here suggest that myf5 upregulation can be a good criterion for the activation of adult myogenesis during X. laevis metamorphosis.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Yamane H
Department of Biological Science, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-0854, Japan.
Nishikawa A
No affiliation info available

MeSH

AnimalsCell DifferentiationGene Expression Regulation, DevelopmentalLarvaLimb BudsMetamorphosis, BiologicalMuscle DevelopmentMuscle, SkeletalMyogenic Regulatory Factor 5NotochordPAX7 Transcription FactorUp-RegulationXenopus ProteinsXenopus laevis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23708921

Citation

Yamane, Hitomi, and Akio Nishikawa. "Differential Muscle Regulatory Factor Gene Expression Between Larval and Adult Myogenesis in the Frog Xenopus Laevis: Adult Myogenic Cell-specific Myf5 Upregulation and Its Relation to the Notochord Suppression of Adult Muscle Differentiation." In Vitro Cellular & Developmental Biology. Animal, vol. 49, no. 7, 2013, pp. 524-36.
Yamane H, Nishikawa A. Differential muscle regulatory factor gene expression between larval and adult myogenesis in the frog Xenopus laevis: adult myogenic cell-specific myf5 upregulation and its relation to the notochord suppression of adult muscle differentiation. In Vitro Cell Dev Biol Anim. 2013;49(7):524-36.
Yamane, H., & Nishikawa, A. (2013). Differential muscle regulatory factor gene expression between larval and adult myogenesis in the frog Xenopus laevis: adult myogenic cell-specific myf5 upregulation and its relation to the notochord suppression of adult muscle differentiation. In Vitro Cellular & Developmental Biology. Animal, 49(7), 524-36. https://doi.org/10.1007/s11626-013-9635-z
Yamane H, Nishikawa A. Differential Muscle Regulatory Factor Gene Expression Between Larval and Adult Myogenesis in the Frog Xenopus Laevis: Adult Myogenic Cell-specific Myf5 Upregulation and Its Relation to the Notochord Suppression of Adult Muscle Differentiation. In Vitro Cell Dev Biol Anim. 2013;49(7):524-36. PubMed PMID: 23708921.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential muscle regulatory factor gene expression between larval and adult myogenesis in the frog Xenopus laevis: adult myogenic cell-specific myf5 upregulation and its relation to the notochord suppression of adult muscle differentiation. AU - Yamane,Hitomi, AU - Nishikawa,Akio, Y1 - 2013/05/25/ PY - 2012/12/16/received PY - 2013/05/06/accepted PY - 2013/5/28/entrez PY - 2013/5/28/pubmed PY - 2014/3/13/medline SP - 524 EP - 36 JF - In vitro cellular & developmental biology. Animal JO - In Vitro Cell Dev Biol Anim VL - 49 IS - 7 N2 - During Xenopus laevis metamorphosis, larval-to-adult muscle conversion depends on the differential responses of adult and larval myogenic cells to thyroid hormone. Essential differences in cell growth, differentiation, and hormone-dependent life-or-death fate have been reported between cultured larval (tail) and adult (hindlimb) myogenic cells. A previous study revealed that tail notochord cells suppress terminal differentiation in adult (but not larval) myogenic cells. However, little is known about the differences in expression patterns of myogenic regulatory factors (MRF) and the satellite cell marker Pax7 between adult and larval myogenic cells. In the present study, we compared mRNA expression of these factors between the two types. At first, reverse transcription polymerase chain reaction analysis of hindlimb buds showed sequential upregulation of myf5, myogenin, myod, and mrf4 during stages 50-54, when limb buds elongate and muscles begin to form. By contrast, in the tail, there was no such increase during the same period. Secondary, these results were duplicated in vitro: adult myogenic cells upregulated myf5, myod, and pax7 in the early culture period, followed by myogenin upregulation and myotube differentiation, while larval myogenic cells did not upregulate these genes and precociously started myotube differentiation. Thirdly, myf5 upregulation and early-phase proliferation in adult myogenic cells were potently inhibited by the presence of notochord cells, suggesting that notochord cells suppress adult myogenesis through inhibiting the transition from Myf5(-) stem cells to Myf5(+) committed myoblasts. All of the data presented here suggest that myf5 upregulation can be a good criterion for the activation of adult myogenesis during X. laevis metamorphosis. SN - 1543-706X UR - https://www.unboundmedicine.com/medline/citation/23708921/Differential_muscle_regulatory_factor_gene_expression_between_larval_and_adult_myogenesis_in_the_frog_Xenopus_laevis:_adult_myogenic_cell_specific_myf5_upregulation_and_its_relation_to_the_notochord_suppression_of_adult_muscle_differentiation_ L2 - https://dx.doi.org/10.1007/s11626-013-9635-z DB - PRIME DP - Unbound Medicine ER -