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First isolation of Mimivirus in a patient with pneumonia.
Clin Infect Dis. 2013 Aug; 57(4):e127-34.CI

Abstract

BACKGROUND

Mimiviridae Mimivirus, including the largest known viruses, multiply in amoebae. Mimiviruses have been linked to pneumonia, but they have never been isolated from patients. To further understand the pathogenic role of these viruses, we aimed to isolate them from a patient presenting with pneumonia.

METHODS

We cultured, on Acanthamoeba polyphaga amoebae, pulmonary samples from 196 Tunisian patients with community-acquired pneumonia during the period 2009-2010. An improved technique was used for Mimivirus isolation, which used agar plates where the growth of giant viruses is revealed by the formation of lysis plaques. Mimivirus serology was tested by microimmunofluorescence and by bidimensional immunoproteomic analysis using Mimivirus strains, to identify specific immunoreactive proteins. The new Mimivirus strain genome sequencing was performed on Roche 454 GS FLX Titanium, then AB SOLiD instruments.

RESULTS

We successfully isolated a Mimivirus (LBA111), the largest virus ever isolated in a human sample, from a 72-year-old woman presenting with pneumonia. Electron microscopy revealed a Mimivirus-like virion with a size of 554 ± 10 nm. The LBA111 genome is 1.23 megabases, and it is closely related to that of Megavirus chilensis. Furthermore, the serum from the patient reacted specifically to the virus compared to controls.

CONCLUSIONS

This is the first Mimivirus isolated from a human specimen. The findings presented above together with previous works establish that mimiviruses can be associated with pneumonia. The common occurrence of these viruses in water and soil makes them probable global agents that are worthy of investigation.

Authors+Show Affiliations

Aix Marseille Université, Unité de Recherche sur le Maladies Infectieuses et Tropicales Emergentes, UM63, Centre National de la Recherche Scientifique 7278, Institut de Recherche pour le Développement 198, Institut National de la Santé et de la Recherche Médicale 1095, Marseille, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23709652

Citation

Saadi, Hanene, et al. "First Isolation of Mimivirus in a Patient With Pneumonia." Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America, vol. 57, no. 4, 2013, pp. e127-34.
Saadi H, Pagnier I, Colson P, et al. First isolation of Mimivirus in a patient with pneumonia. Clin Infect Dis. 2013;57(4):e127-34.
Saadi, H., Pagnier, I., Colson, P., Cherif, J. K., Beji, M., Boughalmi, M., Azza, S., Armstrong, N., Robert, C., Fournous, G., La Scola, B., & Raoult, D. (2013). First isolation of Mimivirus in a patient with pneumonia. Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America, 57(4), e127-34. https://doi.org/10.1093/cid/cit354
Saadi H, et al. First Isolation of Mimivirus in a Patient With Pneumonia. Clin Infect Dis. 2013;57(4):e127-34. PubMed PMID: 23709652.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - First isolation of Mimivirus in a patient with pneumonia. AU - Saadi,Hanene, AU - Pagnier,Isabelle, AU - Colson,Philippe, AU - Cherif,Jouda Kanoun, AU - Beji,Majed, AU - Boughalmi,Mondher, AU - Azza,Saïd, AU - Armstrong,Nicholas, AU - Robert,Catherine, AU - Fournous,Ghislain, AU - La Scola,Bernard, AU - Raoult,Didier, Y1 - 2013/05/24/ PY - 2013/5/28/entrez PY - 2013/5/28/pubmed PY - 2014/2/8/medline KW - Mimivirus KW - giant virus KW - human virus KW - pneumonia SP - e127 EP - 34 JF - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America JO - Clin. Infect. Dis. VL - 57 IS - 4 N2 - BACKGROUND: Mimiviridae Mimivirus, including the largest known viruses, multiply in amoebae. Mimiviruses have been linked to pneumonia, but they have never been isolated from patients. To further understand the pathogenic role of these viruses, we aimed to isolate them from a patient presenting with pneumonia. METHODS: We cultured, on Acanthamoeba polyphaga amoebae, pulmonary samples from 196 Tunisian patients with community-acquired pneumonia during the period 2009-2010. An improved technique was used for Mimivirus isolation, which used agar plates where the growth of giant viruses is revealed by the formation of lysis plaques. Mimivirus serology was tested by microimmunofluorescence and by bidimensional immunoproteomic analysis using Mimivirus strains, to identify specific immunoreactive proteins. The new Mimivirus strain genome sequencing was performed on Roche 454 GS FLX Titanium, then AB SOLiD instruments. RESULTS: We successfully isolated a Mimivirus (LBA111), the largest virus ever isolated in a human sample, from a 72-year-old woman presenting with pneumonia. Electron microscopy revealed a Mimivirus-like virion with a size of 554 ± 10 nm. The LBA111 genome is 1.23 megabases, and it is closely related to that of Megavirus chilensis. Furthermore, the serum from the patient reacted specifically to the virus compared to controls. CONCLUSIONS: This is the first Mimivirus isolated from a human specimen. The findings presented above together with previous works establish that mimiviruses can be associated with pneumonia. The common occurrence of these viruses in water and soil makes them probable global agents that are worthy of investigation. SN - 1537-6591 UR - https://www.unboundmedicine.com/medline/citation/23709652/First_isolation_of_Mimivirus_in_a_patient_with_pneumonia_ L2 - https://academic.oup.com/cid/article-lookup/doi/10.1093/cid/cit354 DB - PRIME DP - Unbound Medicine ER -