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[Tumor infiltrating regulatory T cells in human breast cancer and associated draining lymph nodes: an in-situ analysis].
Zhonghua Bing Li Xue Za Zhi 2013; 42(2):95-100ZB

Abstract

OBJECTIVE

To retrospectively analyze the quantity and status of the tumor infiltrating regulatory T lymphocytes in breast cancer and the draining lymph nodes, and to elucidate the clinical pathologic significance.

METHODS

Seventy-four breast cancer samples with excised axillary lymph nodes were typed and staged histopathologically. The regulatory T lymphocytes were labeled by immunohistochemistry using EnVision method with the monoclonal antibodies against CD25 and Foxp3, and the immunophenotype was analyzed. In addition, the expression of IFN-γ, IL-10 and TGF-β1 mRNA in lymphocytes of lymph nodes draining the tumors was detected by in situ hybridization with the corresponding specific oligo nucleaic acid probes.

RESULTS

The number of CD25(+)Foxp3(+) T cells infiltrating the interstitium was much higher than that in the parenchymal tissue of the cancer. In the tumor draining lymph nodes, CD25(+) cells and Foxp3(+) cells were predominantly distributed in the paracortex with a proliferative pattern. TGF-β1, INF-γ and IL-10 mRNA positive cells showed a similar distribution pattern in the draining lymph nodes. Among the 39 cases with metastatic disease, the lymph nodes with metastases showed a much higher number of CD25(+)Foxp3(+) cells than that without metastases (23.5 vs 17.3 and 23.8 vs 15.5; P < 0.05). However, there was no difference in the density of Foxp3(+)CD25(+) cells in the draining lymph nodes between the death and survival groups (P > 0.05). Cytokine expression of TGF-β1, IL-10 and IFN-γ mRNA in the lymphocytes of draining lymph nodes in 24 cases showed that there were more IL-10 mRNA positive cells in the dead patients than that in the survived patients. A similar trend was observed for TGF-β1 mRNA positive cells but the difference was not statistically significant (P > 0.05). The expression rate of TGF-β1 and IL-10 mRNA in the draining lymph nodes was proportional to that of CD25(+) and Foxp3(+) cells (P < 0.05), and the expression of TGF-β1 positive cells was also proportional to that of IL-10 mRNA positive cells (P < 0.01). The expression of IFN-γ mRNA among these groups showed no significance (P > 0.05).

CONCLUSIONS

Regulatory T cells may play important roles in inhibiting the host antitumor immunity, and the presence of increased regulatory T cells and Th2-secreting cells in paracortex with a proliferative pattern in the tumor draining lymph nodes implies that the paracortical proliferation of draining lymph nodes may not reflect positive antitumor effects.

Authors+Show Affiliations

Xi'an Jiaotong University, Xi'an, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

23710915

Citation

Wang, Hong-yan, et al. "[Tumor Infiltrating Regulatory T Cells in Human Breast Cancer and Associated Draining Lymph Nodes: an In-situ Analysis]." Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology, vol. 42, no. 2, 2013, pp. 95-100.
Wang HY, Shi QF, Sun Y, et al. [Tumor infiltrating regulatory T cells in human breast cancer and associated draining lymph nodes: an in-situ analysis]. Zhonghua Bing Li Xue Za Zhi. 2013;42(2):95-100.
Wang, H. Y., Shi, Q. F., Sun, Y., He, J. J., & Wang, Y. L. (2013). [Tumor infiltrating regulatory T cells in human breast cancer and associated draining lymph nodes: an in-situ analysis]. Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology, 42(2), pp. 95-100. doi:10.3760/cma.j.issn.0529-5807.2013.02.006.
Wang HY, et al. [Tumor Infiltrating Regulatory T Cells in Human Breast Cancer and Associated Draining Lymph Nodes: an In-situ Analysis]. Zhonghua Bing Li Xue Za Zhi. 2013;42(2):95-100. PubMed PMID: 23710915.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Tumor infiltrating regulatory T cells in human breast cancer and associated draining lymph nodes: an in-situ analysis]. AU - Wang,Hong-yan, AU - Shi,Qin-feng, AU - Sun,Ying, AU - He,Jian-jun, AU - Wang,Yi-li, PY - 2013/5/29/entrez PY - 2013/5/29/pubmed PY - 2013/9/17/medline SP - 95 EP - 100 JF - Zhonghua bing li xue za zhi = Chinese journal of pathology JO - Zhonghua Bing Li Xue Za Zhi VL - 42 IS - 2 N2 - OBJECTIVE: To retrospectively analyze the quantity and status of the tumor infiltrating regulatory T lymphocytes in breast cancer and the draining lymph nodes, and to elucidate the clinical pathologic significance. METHODS: Seventy-four breast cancer samples with excised axillary lymph nodes were typed and staged histopathologically. The regulatory T lymphocytes were labeled by immunohistochemistry using EnVision method with the monoclonal antibodies against CD25 and Foxp3, and the immunophenotype was analyzed. In addition, the expression of IFN-γ, IL-10 and TGF-β1 mRNA in lymphocytes of lymph nodes draining the tumors was detected by in situ hybridization with the corresponding specific oligo nucleaic acid probes. RESULTS: The number of CD25(+)Foxp3(+) T cells infiltrating the interstitium was much higher than that in the parenchymal tissue of the cancer. In the tumor draining lymph nodes, CD25(+) cells and Foxp3(+) cells were predominantly distributed in the paracortex with a proliferative pattern. TGF-β1, INF-γ and IL-10 mRNA positive cells showed a similar distribution pattern in the draining lymph nodes. Among the 39 cases with metastatic disease, the lymph nodes with metastases showed a much higher number of CD25(+)Foxp3(+) cells than that without metastases (23.5 vs 17.3 and 23.8 vs 15.5; P < 0.05). However, there was no difference in the density of Foxp3(+)CD25(+) cells in the draining lymph nodes between the death and survival groups (P > 0.05). Cytokine expression of TGF-β1, IL-10 and IFN-γ mRNA in the lymphocytes of draining lymph nodes in 24 cases showed that there were more IL-10 mRNA positive cells in the dead patients than that in the survived patients. A similar trend was observed for TGF-β1 mRNA positive cells but the difference was not statistically significant (P > 0.05). The expression rate of TGF-β1 and IL-10 mRNA in the draining lymph nodes was proportional to that of CD25(+) and Foxp3(+) cells (P < 0.05), and the expression of TGF-β1 positive cells was also proportional to that of IL-10 mRNA positive cells (P < 0.01). The expression of IFN-γ mRNA among these groups showed no significance (P > 0.05). CONCLUSIONS: Regulatory T cells may play important roles in inhibiting the host antitumor immunity, and the presence of increased regulatory T cells and Th2-secreting cells in paracortex with a proliferative pattern in the tumor draining lymph nodes implies that the paracortical proliferation of draining lymph nodes may not reflect positive antitumor effects. SN - 0529-5807 UR - https://www.unboundmedicine.com/medline/citation/23710915/[Tumor_infiltrating_regulatory_T_cells_in_human_breast_cancer_and_associated_draining_lymph_nodes:_an_in_situ_analysis]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0529-5807&amp;year=2013&amp;vol=42&amp;issue=2&amp;fpage=95 DB - PRIME DP - Unbound Medicine ER -