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[A nalidixic acid-resistant Salmonella enteritidis outbreak in Popayán, Cauca, 2011].
Biomedica. 2013 Jan-Mar; 33(1):62-9.B

Abstract

INTRODUCTION

Salmonella Enteritidis is recognized worldwide as one of the main agents of human gastrointestinal infection. Several reports indicate the presence of isolates with decreased sensitivity to ciprofloxacin that can lead to a delayed response or the development of resistance during treatment.

OBJECTIVE

To describe and characterize isolates of Salmonella Enteritidis associated to an outbreak of food-borne diseases in Popayán, Cauca.

MATERIALS AND METHODS

Ten Salmonella Enteritidis isolates from nine patients and one food sample (chicken sandwich) were analyzed by biochemical tests, serotyping and antimicrobial sensitivity. The minimum inhibitory concentration to ciprofloxacin was determined by E-test and the genetic profile of the isolates was tested by pulsed field gel electrophoresis (PFGE) with XbaI and Blnl enzymes.

RESULTS

Salmonella Enteritidis was identified in all isolates. They were resistant to nalidixic acid and had a decreased sensitivity to ciprofloxaxin between 0.25 and 0.5 μg/ml; all isolates were sensitive to all the other antimicrobials we tested. Ten isolates were grouped by PFGE with the XbaI enzyme in the COIN11.JEG.X01.0038 pattern, and seven isolates were confirmed with the BlnI enzyme using the COIN11.JEG.A26.0009 pattern.

CONCLUSION

We report for the first time an outbreak of nalidixic acid-resistant Salmonella Enteritidis in Colombia and confirmed by phenotypic and genotypic analysis the association between the isolates from patients and the chicken sandwich as the source of infection.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Díaz MÁ
Grupo de Microbiología, Subdirección Red Nacional de Laboratorios, Instituto Nacional de Salud, Bogotá, D.C., Colombia.
Díaz PL
No affiliation info available
Rodríguez EC
No affiliation info available
Montaño LA
No affiliation info available
Gartner DM
No affiliation info available
Vernaza ME
No affiliation info available
Eljach V
No affiliation info available
Realpe ME
No affiliation info available

MeSH

AdolescentAdultAgedAnimalsAnti-Bacterial AgentsBacterial Typing TechniquesChickensChildChild, PreschoolCiprofloxacinColombiaDNA, BacterialDisease OutbreaksDrug Resistance, Multiple, BacterialFemaleFood HandlingFood MicrobiologyHumansHygieneInfantMaleMeatMiddle AgedNalidixic AcidRefrigerationRestaurantsSalmonella Food PoisoningSalmonella enteritidis

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

23715308

Citation

Díaz, Miguel Ángel, et al. "[A Nalidixic Acid-resistant Salmonella Enteritidis Outbreak in Popayán, Cauca, 2011]." Biomedica : Revista Del Instituto Nacional De Salud, vol. 33, no. 1, 2013, pp. 62-9.
Díaz MÁ, Díaz PL, Rodríguez EC, et al. [A nalidixic acid-resistant Salmonella enteritidis outbreak in Popayán, Cauca, 2011]. Biomedica. 2013;33(1):62-9.
Díaz, M. Á., Díaz, P. L., Rodríguez, E. C., Montaño, L. A., Gartner, D. M., Vernaza, M. E., Eljach, V., & Realpe, M. E. (2013). [A nalidixic acid-resistant Salmonella enteritidis outbreak in Popayán, Cauca, 2011]. Biomedica : Revista Del Instituto Nacional De Salud, 33(1), 62-9. https://doi.org/10.1590/S0120-41572013000100008
Díaz MÁ, et al. [A Nalidixic Acid-resistant Salmonella Enteritidis Outbreak in Popayán, Cauca, 2011]. Biomedica. 2013 Jan-Mar;33(1):62-9. PubMed PMID: 23715308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [A nalidixic acid-resistant Salmonella enteritidis outbreak in Popayán, Cauca, 2011]. AU - Díaz,Miguel Ángel, AU - Díaz,Paula Lucía, AU - Rodríguez,Edna Catering, AU - Montaño,Lucy Angeline, AU - Gartner,Doris Mabel, AU - Vernaza,María Elisa, AU - Eljach,Victoria, AU - Realpe,María Elena, PY - 2013/5/30/entrez PY - 2013/5/30/pubmed PY - 2014/2/26/medline SP - 62 EP - 9 JF - Biomedica : revista del Instituto Nacional de Salud JO - Biomedica VL - 33 IS - 1 N2 - INTRODUCTION: Salmonella Enteritidis is recognized worldwide as one of the main agents of human gastrointestinal infection. Several reports indicate the presence of isolates with decreased sensitivity to ciprofloxacin that can lead to a delayed response or the development of resistance during treatment. OBJECTIVE: To describe and characterize isolates of Salmonella Enteritidis associated to an outbreak of food-borne diseases in Popayán, Cauca. MATERIALS AND METHODS: Ten Salmonella Enteritidis isolates from nine patients and one food sample (chicken sandwich) were analyzed by biochemical tests, serotyping and antimicrobial sensitivity. The minimum inhibitory concentration to ciprofloxacin was determined by E-test and the genetic profile of the isolates was tested by pulsed field gel electrophoresis (PFGE) with XbaI and Blnl enzymes. RESULTS: Salmonella Enteritidis was identified in all isolates. They were resistant to nalidixic acid and had a decreased sensitivity to ciprofloxaxin between 0.25 and 0.5 μg/ml; all isolates were sensitive to all the other antimicrobials we tested. Ten isolates were grouped by PFGE with the XbaI enzyme in the COIN11.JEG.X01.0038 pattern, and seven isolates were confirmed with the BlnI enzyme using the COIN11.JEG.A26.0009 pattern. CONCLUSION: We report for the first time an outbreak of nalidixic acid-resistant Salmonella Enteritidis in Colombia and confirmed by phenotypic and genotypic analysis the association between the isolates from patients and the chicken sandwich as the source of infection. SN - 2590-7379 UR - https://www.unboundmedicine.com/medline/citation/23715308/[A_nalidixic_acid_resistant_Salmonella_enteritidis_outbreak_in_Popayán_Cauca_2011]_ L2 - https://medlineplus.gov/antibiotics.html DB - PRIME DP - Unbound Medicine ER -