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[Multiplex PCR strategy for the simultaneous identification of Staphylococcus aureus and detection of staphylococcal enterotoxins in isolates from food poisoning outbreaks].
Biomedica. 2013 Jan-Mar; 33(1):122-7.B

Abstract

INTRODUCTION

Staphylococcal food poisoning is the most frequent type of food poisoning around the world. Staphylococcus aureus enterotoxins cause significant loss of water in the intestinal lumen, followed by vomiting and diarrhea.

OBJECTIVE

To report a fast, reliable and inexpensive strategy based on multiplex PCR for the simultaneous identification of S. aureus and detection of five classical S. aureus enterotoxin genes (sea, seb, sec, sed, see) in Staphylococcus spp. strains isolated from food poisoning outbreaks.

MATERIALS AND METHODS

We analyzed isolates from 12 food poisoning outbreaks occurred in Santa Fe province (Argentina). Isolation and phenotypic characterization were carried out by standard procedures. Genotypic analysis was performed by multiplex PCR, using primers for nuc , sea-see and 16S rRNA genes simultaneously.

RESULTS

Of all the strains tested, 58% were found to carry toxigenic genes. Sea and seb toxins were found at the same percentage (29%) while sec, sed and see genes were found in a lower and identical proportion (14%). We did not find more than one different type of S. aureus enterotoxin in the isolates analyzed.

CONCLUSIONS

The multiplex PCR strategy designed in this work has enabled us to identify strains of S. aureus and detect -at the same time- their enterotoxigenic ability. At present, our efforts are devoted to the detection of genes encoding enterotoxins other than the classical ones, in order to know their impact on staphylococcal food poisoning, as well as to investigate their relevance to our country's public health.

Authors+Show Affiliations

Servicio de Laboratorio, Agencia Santafesina de Seguridad Alimentaria (ASSAL), Santa Fe, Argentina. anibal.brizzio@gmail.comNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

23715315

Citation

Brizzio, Aníbal A., et al. "[Multiplex PCR Strategy for the Simultaneous Identification of Staphylococcus Aureus and Detection of Staphylococcal Enterotoxins in Isolates From Food Poisoning Outbreaks]." Biomedica : Revista Del Instituto Nacional De Salud, vol. 33, no. 1, 2013, pp. 122-7.
Brizzio AA, Tedeschi FA, Zalazar FE. [Multiplex PCR strategy for the simultaneous identification of Staphylococcus aureus and detection of staphylococcal enterotoxins in isolates from food poisoning outbreaks]. Biomedica. 2013;33(1):122-7.
Brizzio, A. A., Tedeschi, F. A., & Zalazar, F. E. (2013). [Multiplex PCR strategy for the simultaneous identification of Staphylococcus aureus and detection of staphylococcal enterotoxins in isolates from food poisoning outbreaks]. Biomedica : Revista Del Instituto Nacional De Salud, 33(1), 122-7. https://doi.org/10.1590/S0120-41572013000100015
Brizzio AA, Tedeschi FA, Zalazar FE. [Multiplex PCR Strategy for the Simultaneous Identification of Staphylococcus Aureus and Detection of Staphylococcal Enterotoxins in Isolates From Food Poisoning Outbreaks]. Biomedica. 2013 Jan-Mar;33(1):122-7. PubMed PMID: 23715315.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Multiplex PCR strategy for the simultaneous identification of Staphylococcus aureus and detection of staphylococcal enterotoxins in isolates from food poisoning outbreaks]. AU - Brizzio,Aníbal A, AU - Tedeschi,Fabián A, AU - Zalazar,Fabián E, PY - 2011/11/11/received PY - 2011/08/24/accepted PY - 2013/5/30/entrez PY - 2013/5/30/pubmed PY - 2014/2/26/medline SP - 122 EP - 7 JF - Biomedica : revista del Instituto Nacional de Salud JO - Biomedica VL - 33 IS - 1 N2 - INTRODUCTION: Staphylococcal food poisoning is the most frequent type of food poisoning around the world. Staphylococcus aureus enterotoxins cause significant loss of water in the intestinal lumen, followed by vomiting and diarrhea. OBJECTIVE: To report a fast, reliable and inexpensive strategy based on multiplex PCR for the simultaneous identification of S. aureus and detection of five classical S. aureus enterotoxin genes (sea, seb, sec, sed, see) in Staphylococcus spp. strains isolated from food poisoning outbreaks. MATERIALS AND METHODS: We analyzed isolates from 12 food poisoning outbreaks occurred in Santa Fe province (Argentina). Isolation and phenotypic characterization were carried out by standard procedures. Genotypic analysis was performed by multiplex PCR, using primers for nuc , sea-see and 16S rRNA genes simultaneously. RESULTS: Of all the strains tested, 58% were found to carry toxigenic genes. Sea and seb toxins were found at the same percentage (29%) while sec, sed and see genes were found in a lower and identical proportion (14%). We did not find more than one different type of S. aureus enterotoxin in the isolates analyzed. CONCLUSIONS: The multiplex PCR strategy designed in this work has enabled us to identify strains of S. aureus and detect -at the same time- their enterotoxigenic ability. At present, our efforts are devoted to the detection of genes encoding enterotoxins other than the classical ones, in order to know their impact on staphylococcal food poisoning, as well as to investigate their relevance to our country's public health. SN - 2590-7379 UR - https://www.unboundmedicine.com/medline/citation/23715315/[Multiplex_PCR_strategy_for_the_simultaneous_identification_of_Staphylococcus_aureus_and_detection_of_staphylococcal_enterotoxins_in_isolates_from_food_poisoning_outbreaks]_ L2 - http://www.diseaseinfosearch.org/result/6858 DB - PRIME DP - Unbound Medicine ER -