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Protective effects of two Lactobacillus plantarum strains in hyperlipidemic mice.
World J Gastroenterol 2013; 19(20):3150-6WJ

Abstract

AIM

To investigate the effects of Lactobacillus plantarum (L. plantarum) CAI6 and L. plantarum SC4 on hyperlipidemic mice.

METHODS

Male Kunming mice were fed a high-cholesterol diet for 28 d to construct hyperlipidemic models. Hyperlipidemic mice and normal mice were assigned to 3 groups which were separately treated with L. plantarum CAI6, L. plantarum SC4, and physiological saline through oral gavage for 28 d. Total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels were measured by commercially available enzyme kits. FACS Calibur flow cytometry was used to examine hepatic and renal nuclear factor-erythroid 2-related factor 2 (Nrf2) expression. The morphology of livers was checked by hematoxylin and eosin staining and optical microscope observation.

RESULTS

Compared with normal mice, hyperlipidemic mice possessed significantly higher TC (3.50 ± 0.43 vs 2.89 ± 0.36, P < 0.01), TG (1.76 ± 0.07 vs 1.10 ± 0.16, P < 0.01), and LDL-C (1.72 ± 0.20 vs 0.82 ± 0.10, P< 0.01) levels, resulting in an increase of atherogenic index (AI) (2.34 ± 1.60 vs 0.93 ± 0.55, P < 0.05) and LDL-C/HDL-C ratio (1.43 ± 0.12 vs 0.51 ± 0.16, P < 0.05). After treatment with L. plantarum CAI6/L. plantarum SC4, TG (1.43 ± 0.27/1.54 ± 0.10 vs 1.76 ± 0.07, P < 0.01/P < 0.05) and LDL-C (1.42 ± 0.07/1.47 ± 0.12 vs 1.72 ± 0.20, P < 0.01/P < 0.01) in hyperlipidemic mice significantly decreased. In addition, TC, HDL-C, AI, and LDL-C/HDL-C ratio were all positively changed. Meanwhile, the treatment markedly alleviated hepatic steatosis and significantly stimulated Nrf2 expression (73.79 ± 0.80/72.96 ± 1.22 vs 54.94 ± 1.84, P < 0.01/P < 0.01) in hepatocytes of hyperlipidemic mice.

CONCLUSION

L. plantarum CAI6 and L. plantarum SC4 may protect against cardiovascular disease by lipid metabolism regulation and Nrf2-induced antioxidative defense in hyperlipidemic mice.

Authors+Show Affiliations

Department of Biological Engineering, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, Hebei Province, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23716997

Citation

Wang, Li-Xin, et al. "Protective Effects of Two Lactobacillus Plantarum Strains in Hyperlipidemic Mice." World Journal of Gastroenterology, vol. 19, no. 20, 2013, pp. 3150-6.
Wang LX, Liu K, Gao DW, et al. Protective effects of two Lactobacillus plantarum strains in hyperlipidemic mice. World J Gastroenterol. 2013;19(20):3150-6.
Wang, L. X., Liu, K., Gao, D. W., & Hao, J. K. (2013). Protective effects of two Lactobacillus plantarum strains in hyperlipidemic mice. World Journal of Gastroenterology, 19(20), pp. 3150-6. doi:10.3748/wjg.v19.i20.3150.
Wang LX, et al. Protective Effects of Two Lactobacillus Plantarum Strains in Hyperlipidemic Mice. World J Gastroenterol. 2013 May 28;19(20):3150-6. PubMed PMID: 23716997.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of two Lactobacillus plantarum strains in hyperlipidemic mice. AU - Wang,Li-Xin, AU - Liu,Kai, AU - Gao,Da-Wei, AU - Hao,Ji-Kui, PY - 2012/12/07/received PY - 2013/02/03/revised PY - 2013/02/28/accepted PY - 2013/5/30/entrez PY - 2013/5/30/pubmed PY - 2013/12/18/medline KW - Cardiovascular disease KW - Hepatic steatosis KW - Hypolipidemic KW - Lactobacillus plantarum KW - Metabolic syndrome KW - Nuclear factor erythroid 2-related factor 2 SP - 3150 EP - 6 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 19 IS - 20 N2 - AIM: To investigate the effects of Lactobacillus plantarum (L. plantarum) CAI6 and L. plantarum SC4 on hyperlipidemic mice. METHODS: Male Kunming mice were fed a high-cholesterol diet for 28 d to construct hyperlipidemic models. Hyperlipidemic mice and normal mice were assigned to 3 groups which were separately treated with L. plantarum CAI6, L. plantarum SC4, and physiological saline through oral gavage for 28 d. Total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels were measured by commercially available enzyme kits. FACS Calibur flow cytometry was used to examine hepatic and renal nuclear factor-erythroid 2-related factor 2 (Nrf2) expression. The morphology of livers was checked by hematoxylin and eosin staining and optical microscope observation. RESULTS: Compared with normal mice, hyperlipidemic mice possessed significantly higher TC (3.50 ± 0.43 vs 2.89 ± 0.36, P < 0.01), TG (1.76 ± 0.07 vs 1.10 ± 0.16, P < 0.01), and LDL-C (1.72 ± 0.20 vs 0.82 ± 0.10, P< 0.01) levels, resulting in an increase of atherogenic index (AI) (2.34 ± 1.60 vs 0.93 ± 0.55, P < 0.05) and LDL-C/HDL-C ratio (1.43 ± 0.12 vs 0.51 ± 0.16, P < 0.05). After treatment with L. plantarum CAI6/L. plantarum SC4, TG (1.43 ± 0.27/1.54 ± 0.10 vs 1.76 ± 0.07, P < 0.01/P < 0.05) and LDL-C (1.42 ± 0.07/1.47 ± 0.12 vs 1.72 ± 0.20, P < 0.01/P < 0.01) in hyperlipidemic mice significantly decreased. In addition, TC, HDL-C, AI, and LDL-C/HDL-C ratio were all positively changed. Meanwhile, the treatment markedly alleviated hepatic steatosis and significantly stimulated Nrf2 expression (73.79 ± 0.80/72.96 ± 1.22 vs 54.94 ± 1.84, P < 0.01/P < 0.01) in hepatocytes of hyperlipidemic mice. CONCLUSION: L. plantarum CAI6 and L. plantarum SC4 may protect against cardiovascular disease by lipid metabolism regulation and Nrf2-induced antioxidative defense in hyperlipidemic mice. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/23716997/Protective_effects_of_two_Lactobacillus_plantarum_strains_in_hyperlipidemic_mice_ L2 - http://www.wjgnet.com/1007-9327/full/v19/i20/3150.htm DB - PRIME DP - Unbound Medicine ER -