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Genome-wide analysis of the salmonella Fis regulon and its regulatory mechanism on pathogenicity islands.
PLoS One. 2013; 8(5):e64688.Plos

Abstract

Fis, one of the most important nucleoid-associated proteins, functions as a global regulator of transcription in bacteria that has been comprehensively studied in Escherichia coli K12. Fis also influences the virulence of Salmonella enterica and pathogenic E. coli by regulating their virulence genes, however, the relevant mechanism is unclear. In this report, using combined RNA-seq and chromatin immunoprecipitation (ChIP)-seq technologies, we first identified 1646 Fis-regulated genes and 885 Fis-binding targets in the S. enterica serovar Typhimurium, and found a Fis regulon different from that in E. coli. Fis has been reported to contribute to the invasion ability of S. enterica. By using cell infection assays, we found it also enhances the intracellular replication ability of S. enterica within macrophage cell, which is of central importance for the pathogenesis of infections. Salmonella pathogenicity islands (SPI)-1 and SPI-2 are crucial for the invasion and survival of S. enterica in host cells. Using mutation and overexpression experiments, real-time PCR analysis, and electrophoretic mobility shift assays, we demonstrated that Fis regulates 63 of the 94 Salmonella pathogenicity island (SPI)-1 and SPI-2 genes, by three regulatory modes: i) binds to SPI regulators in the gene body or in upstream regions; ii) binds to SPI genes directly to mediate transcriptional activation of themselves and downstream genes; iii) binds to gene encoding OmpR which affects SPI gene expression by controlling SPI regulators SsrA and HilD. Our results provide new insights into the impact of Fis on SPI genes and the pathogenicity of S. enterica.

Authors+Show Affiliations

TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, PR China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23717649

Citation

Wang, Hui, et al. "Genome-wide Analysis of the Salmonella Fis Regulon and Its Regulatory Mechanism On Pathogenicity Islands." PloS One, vol. 8, no. 5, 2013, pp. e64688.
Wang H, Liu B, Wang Q, et al. Genome-wide analysis of the salmonella Fis regulon and its regulatory mechanism on pathogenicity islands. PLoS One. 2013;8(5):e64688.
Wang, H., Liu, B., Wang, Q., & Wang, L. (2013). Genome-wide analysis of the salmonella Fis regulon and its regulatory mechanism on pathogenicity islands. PloS One, 8(5), e64688. https://doi.org/10.1371/journal.pone.0064688
Wang H, et al. Genome-wide Analysis of the Salmonella Fis Regulon and Its Regulatory Mechanism On Pathogenicity Islands. PLoS One. 2013;8(5):e64688. PubMed PMID: 23717649.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide analysis of the salmonella Fis regulon and its regulatory mechanism on pathogenicity islands. AU - Wang,Hui, AU - Liu,Bin, AU - Wang,Quan, AU - Wang,Lei, Y1 - 2013/05/23/ PY - 2013/03/11/received PY - 2013/04/17/accepted PY - 2013/5/30/entrez PY - 2013/5/30/pubmed PY - 2014/1/8/medline SP - e64688 EP - e64688 JF - PloS one JO - PLoS One VL - 8 IS - 5 N2 - Fis, one of the most important nucleoid-associated proteins, functions as a global regulator of transcription in bacteria that has been comprehensively studied in Escherichia coli K12. Fis also influences the virulence of Salmonella enterica and pathogenic E. coli by regulating their virulence genes, however, the relevant mechanism is unclear. In this report, using combined RNA-seq and chromatin immunoprecipitation (ChIP)-seq technologies, we first identified 1646 Fis-regulated genes and 885 Fis-binding targets in the S. enterica serovar Typhimurium, and found a Fis regulon different from that in E. coli. Fis has been reported to contribute to the invasion ability of S. enterica. By using cell infection assays, we found it also enhances the intracellular replication ability of S. enterica within macrophage cell, which is of central importance for the pathogenesis of infections. Salmonella pathogenicity islands (SPI)-1 and SPI-2 are crucial for the invasion and survival of S. enterica in host cells. Using mutation and overexpression experiments, real-time PCR analysis, and electrophoretic mobility shift assays, we demonstrated that Fis regulates 63 of the 94 Salmonella pathogenicity island (SPI)-1 and SPI-2 genes, by three regulatory modes: i) binds to SPI regulators in the gene body or in upstream regions; ii) binds to SPI genes directly to mediate transcriptional activation of themselves and downstream genes; iii) binds to gene encoding OmpR which affects SPI gene expression by controlling SPI regulators SsrA and HilD. Our results provide new insights into the impact of Fis on SPI genes and the pathogenicity of S. enterica. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23717649/Genome_wide_analysis_of_the_salmonella_Fis_regulon_and_its_regulatory_mechanism_on_pathogenicity_islands_ L2 - https://dx.plos.org/10.1371/journal.pone.0064688 DB - PRIME DP - Unbound Medicine ER -