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The joint in vitro action of polymyxin B and miconazole against pathogens associated with canine otitis externa from three European countries.
Vet Dermatol. 2013 Aug; 24(4):439-45, e96-7.VD

Abstract

BACKGROUND

Canine otitis externa, an inflammation of the external ear canal, can be maintained and worsened by bacterial or fungal infections. For topical treatment, combinations of anti-inflammatory and antimicrobial ingredients are mainly used.

HYPOTHESIS/OBJECTIVES

This study was conducted to elucidate the in vitro activity of polymyxin B and miconazole against clinical bacterial isolates from three European countries, to investigate possible differences in sensitivity and to assess drug interactions.

ANIMALS

Seventeen strains of Escherichia coli, 24 strains of Pseudomonas aeruginosa, 24 strains of Proteus mirabilis and 25 strains of Staphylococcus pseudintermedius from dogs with diagnosed otitis externa had been isolated in Germany, France and Italy.

METHODS

Drug activities were evaluated by minimal inhibitory concentration (MIC) and minimal bactericidal concentration. The potentiation of polymyxin B plus miconazole was calculated using the fractional inhibitory concentration index (FICI). An FICI ≤0.5 defined synergy. Furthermore, geographical variations in the FICI and MIC were assessed by statistical analysis.

RESULTS

Bacterial susceptibilities were comparable in different European countries, because there were no significant MIC and FICI variations (P > 0.05). As a single agent, polymyxin B had bactericidal activity against most E. coli and P. aeruginosa strains and, in higher concentrations, against S. pseudintermedius strains. Miconazole was bactericidal against all Staphylococcus strains. Synergy was demonstrated against strains of E. coli and P. aeruginosa (FICI = 0.25 and 0.50, respectively), whereas overall there was no interaction against S. pseudintermedius strains (FICI = 1.25). Proteus mirabilis strains were not inhibited by each of the drugs individually or by their combination.

CONCLUSIONS AND CLINICAL IMPORTANCE

In vitro synergy of polymyxin B and miconazole against E. coli and P. aeruginosa isolates indicates a rationale for applying both agents in combination to treat otitis externa when infected with these types of bacteria.

Authors+Show Affiliations

Charité - Universitätsmedizin Berlin, D-10117 Berlin, Germany. sylvia.pietschmann@charite.deNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23721182

Citation

Pietschmann, Silvia, et al. "The Joint in Vitro Action of Polymyxin B and Miconazole Against Pathogens Associated With Canine Otitis Externa From Three European Countries." Veterinary Dermatology, vol. 24, no. 4, 2013, pp. 439-45, e96-7.
Pietschmann S, Meyer M, Voget M, et al. The joint in vitro action of polymyxin B and miconazole against pathogens associated with canine otitis externa from three European countries. Vet Dermatol. 2013;24(4):439-45, e96-7.
Pietschmann, S., Meyer, M., Voget, M., & Cieslicki, M. (2013). The joint in vitro action of polymyxin B and miconazole against pathogens associated with canine otitis externa from three European countries. Veterinary Dermatology, 24(4), 439-45, e96-7. https://doi.org/10.1111/vde.12037
Pietschmann S, et al. The Joint in Vitro Action of Polymyxin B and Miconazole Against Pathogens Associated With Canine Otitis Externa From Three European Countries. Vet Dermatol. 2013;24(4):439-45, e96-7. PubMed PMID: 23721182.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The joint in vitro action of polymyxin B and miconazole against pathogens associated with canine otitis externa from three European countries. AU - Pietschmann,Silvia, AU - Meyer,Michael, AU - Voget,Michael, AU - Cieslicki,Michael, Y1 - 2013/05/31/ PY - 2013/04/15/accepted PY - 2013/6/1/entrez PY - 2013/6/1/pubmed PY - 2014/3/13/medline SP - 439-45, e96-7 JF - Veterinary dermatology JO - Vet. Dermatol. VL - 24 IS - 4 N2 - BACKGROUND: Canine otitis externa, an inflammation of the external ear canal, can be maintained and worsened by bacterial or fungal infections. For topical treatment, combinations of anti-inflammatory and antimicrobial ingredients are mainly used. HYPOTHESIS/OBJECTIVES: This study was conducted to elucidate the in vitro activity of polymyxin B and miconazole against clinical bacterial isolates from three European countries, to investigate possible differences in sensitivity and to assess drug interactions. ANIMALS: Seventeen strains of Escherichia coli, 24 strains of Pseudomonas aeruginosa, 24 strains of Proteus mirabilis and 25 strains of Staphylococcus pseudintermedius from dogs with diagnosed otitis externa had been isolated in Germany, France and Italy. METHODS: Drug activities were evaluated by minimal inhibitory concentration (MIC) and minimal bactericidal concentration. The potentiation of polymyxin B plus miconazole was calculated using the fractional inhibitory concentration index (FICI). An FICI ≤0.5 defined synergy. Furthermore, geographical variations in the FICI and MIC were assessed by statistical analysis. RESULTS: Bacterial susceptibilities were comparable in different European countries, because there were no significant MIC and FICI variations (P > 0.05). As a single agent, polymyxin B had bactericidal activity against most E. coli and P. aeruginosa strains and, in higher concentrations, against S. pseudintermedius strains. Miconazole was bactericidal against all Staphylococcus strains. Synergy was demonstrated against strains of E. coli and P. aeruginosa (FICI = 0.25 and 0.50, respectively), whereas overall there was no interaction against S. pseudintermedius strains (FICI = 1.25). Proteus mirabilis strains were not inhibited by each of the drugs individually or by their combination. CONCLUSIONS AND CLINICAL IMPORTANCE: In vitro synergy of polymyxin B and miconazole against E. coli and P. aeruginosa isolates indicates a rationale for applying both agents in combination to treat otitis externa when infected with these types of bacteria. SN - 1365-3164 UR - https://www.unboundmedicine.com/medline/citation/23721182/The_joint_in_vitro_action_of_polymyxin_B_and_miconazole_against_pathogens_associated_with_canine_otitis_externa_from_three_European_countries_ L2 - https://doi.org/10.1111/vde.12037 DB - PRIME DP - Unbound Medicine ER -